Phytocompounds curcumin, quercetin, indole‐3‐carbinol, and resveratrol modulate lactate–pyruvate level along with cytotoxic activity in HeLa cervical cancer cells

2020 ◽  
Author(s):  
Sarita Pani ◽  
Amrita Sahoo ◽  
Abhilipsa Patra ◽  
Priya Ranjan Debata
Keyword(s):  
Cervical Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Cervical Cancer Cells ◽  
Pyruvate Level

scholarly journals Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalisL.), Turmeric (Curcuma longaL.), and Ginger (Zingiber officinaleR.) Essential Oils in Cervical Cancer Cells (HeLa)

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
P. A. S. R. Santos ◽  
G. B. Avanço ◽  
S. B. Nerilo ◽  
R. I. A. Marcelino ◽  
V. Janeiro ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Death ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Hela Cells ◽  
Membrane Integrity ◽  
Annexin V ◽  
Cervical Cancer Cells

The objective of this study was to evaluate the cytotoxic activity of rosemary (REO,Rosmarinus officinalisL.), turmeric (CEO,Curcuma longaL.), and ginger (GEO,Zingiber officinaleR.) essential oils in HeLa cells. Cytotoxicity tests were performedin vitro, using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis. CEO and GEO exhibited potent cytotoxic activity against HeLa cells. IC50obtained was 36.6 μg/mL for CEO and 129.9 μg/mL for GEO. The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 μg/mL of CEO and 32.12 μg/mL of GEO. The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO. The results indicate cytotoxic activityin vitrofor CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells.

Chemistry, chemical biology and photophysics of certain new chromene–triazole–coumarin triads as fluorescent inhibitors of CDK2 and CDK4 induced cancers

2019 ◽  
Vol 43 (35) ◽  
pp. 13863-13872 ◽  
Author(s):  
E. P. Shyam Shankar ◽  
Damodaran Bahulayan
Keyword(s):  
Cervical Cancer ◽  
Solid State ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Chemical Biology ◽  
Solution State ◽  
Cervical Cancer Cells ◽  
Solid State Fluorescence ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

The chromene–triazole–coumarin triads synthesized through the MCR-Click strategy possess intense solution state fluorescence, intense solid-state fluorescence and CDK2/CDK4 targeted cytotoxic activity against human cervical cancer cells (HeLa).

Synthesis and Cytotoxic Activity of Salicyloyl Hydrazone Derivatives

2013 ◽  
Vol 320 ◽  
pp. 522-525
Author(s):  
Xiao Yang Qiu ◽  
An Ran Shi ◽  
Xiao Li Zhang
Keyword(s):  
Cervical Cancer ◽  
Melting Point ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Hela Cells ◽  
Elemental Analyses ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

Three salicyloyl hydrazone derivatives (compounds 1-3) were prepared by reacting salicyloyl hydrazine with substituted formaldehydes. Their structures were characterized by melting point, 1H-NMR, ESI-MS and elemental analyses. The cytotoxic activity of compounds 1-3 was evaluated in vitro against Hela cells (human cervical cancer cells). The results revealed that all the compounds showed cytotoxic activity, with IC50 values lower than 15 μM.

scholarly journals Heme oxygenase and indoleamine regulation by cytokines in cervical cancer cells and natural killer cells cytotoxic activity

2019 ◽  
Vol 16 (5) ◽  
Author(s):  
Pablo C. Ortiz-Lazareno ◽  
Paulina Gómez-Lomeli ◽  
Georgina Hernández-Flores ◽  
Cristina Rodríguez-Padilla ◽  
Francisco Javier Ochoa-Carrillo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Natural Killer Cells ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Natural Killer ◽  
Heme Oxygenase ◽  
Killer Cells ◽  
Cervical Cancer Cells

Water-soluble amphiphilic ruthenium(ii) polypyridyl complexes as potential light-activated therapeutic agents

2020 ◽  
Vol 56 (65) ◽  
pp. 9332-9335
Author(s):  
Sandra Estalayo-Adrián ◽  
Salvador Blasco ◽  
Sandra A. Bright ◽  
Gavin J. McManus ◽  
Guillermo Orellana ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Polypyridyl Complexes ◽  
Cervical Cancer Cells

Two new water-soluble amphiphilic Ru(ii) polypyridyl complexes were synthesised and their photophysical and photobiological properties evaluated; both complexes showed a rapid cellular uptake and phototoxicity against HeLa cervical cancer cells.

The Antitumor Efficiency of Zinc Finger Nuclease Combined with Cisplatin and Trichostatin A in Cervical Cancer Cells

2020 ◽  
Vol 20 (17) ◽  
pp. 2125-2135
Author(s):  
Ci Ren ◽  
Chun Gao ◽  
Xiaomin Li ◽  
Jinfeng Xiong ◽  
Hui Shen ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Zinc Finger ◽  
Hela Cells ◽  
Colony Formation ◽  
Trichostatin A ◽  
Combined Therapy ◽  
Hpv E7 ◽  
Anti Cancer ◽  
Cervical Cancer Cells

Background: Persistent infection with the high-risk of human papillomavirus (HR-HPVs) is the primary etiological factor of cervical cancer; HR-HPVs express oncoproteins E6 and E7, both of which play key roles in the progression of cervical carcinogenesis. Zinc Finger Nucleases (ZFNs) targeting HPV E7 induce specific shear of the E7 gene, weakening the malignant biological effects, hence showing great potential for clinical transformation. Objective: Our aim was to develop a new comprehensive therapy for better clinical application of ZFNs. We here explored the anti-cancer efficiency of HPV targeted ZFNs combined with a platinum-based antineoplastic drug Cisplatin (DDP) and an HDAC inhibitor Trichostatin A (TSA). Methods: SiHa and HeLa cells were exposed to different concentrations of DDP and TSA; the appropriate concentrations for the following experiments were screened according to cell apoptosis. Then cells were grouped for combined or separate treatments; apoptosis, cell viability and proliferation ability were measured by flow cytometry detection, CCK-8 assays and colony formation assays. The xenograft experiments were also performed to determine the anti-cancer effects of the combined therapy. In addition, the HPV E7 and RB1 expressions were measured by western blot analysis. Results: Results showed that the combined therapy induced about two times more apoptosis than that of ZFNs alone in SiHa and HeLa cells, and much more inhibition of cell viability than either of the separate treatment. The colony formation ability was inhibited more than 80% by the co-treatment, the protein expression of HPV16/18E7 was down regulated and that of RB1 was elevated. In addition, the xenografts experiment showed a synergistic effect between DDP and TSA together with ZFNs. Conclusion: Our results demonstrated that ZFNs combined with DDP or TSA functioned effectively in cervical cancer cells, and it provided novel ideas for the prevention and treatment of HPV-related cervical malignancies.

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