Inoculation concentration modulating the secretion and accumulation pattern of exopolysaccharides in desert cyanobacterium Microcoleus vaginatus

2020 ◽  
Author(s):  
Long Qian ◽  
Li Wu ◽  
Lie Yang ◽  
Zulin Zhang
Keyword(s):  
Accumulation Pattern ◽  
Microcoleus Vaginatus

scholarly journals Explore the gene network regulating the composition of fatty acids in cottonseed

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lihong Ma ◽  
Xinqi Cheng ◽  
Chuan Wang ◽  
Xinyu Zhang ◽  
Fei Xue ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Gene Network ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Accumulation Pattern ◽  
Time Points ◽  
Expression Characteristics

Abstract Background Cottonseed is one of the major sources of vegetable oil. Analysis of the dynamic changes of fatty acid components and the genes regulating the composition of fatty acids of cottonseed oil is of great significance for understanding the biological processes underlying biosynthesis of fatty acids and for genetic improving the oil nutritional qualities. Results In this study, we investigated the dynamic relationship of 13 fatty acid components at 12 developmental time points of cottonseed (Gossypium hirsutum L.) and generated cottonseed transcriptome of the 12 time points. At 5–15 day post anthesis (DPA), the contents of polyunsaturated linolenic acid (C18:3n-3) and saturated stearic acid (C18:0) were higher, while linoleic acid (C18:2n-6) was mainly synthesized after 15 DPA. Using 5 DPA as a reference, 15,647 non-redundant differentially expressed genes were identified in 10–60 DPA cottonseed. Co-expression gene network analysis identified six modules containing 3275 genes significantly associated with middle-late seed developmental stages and enriched with genes related to the linoleic acid metabolic pathway and α-linolenic acid metabolism. Genes (Gh_D03G0588 and Gh_A02G1788) encoding stearoyl-ACP desaturase were identified as hub genes and significantly up-regulated at 25 DPA. They seemed to play a decisive role in determining the ratio of saturated fatty acids to unsaturated fatty acids. FAD2 genes (Gh_A13G1850 and Gh_D13G2238) were highly expressed at 25–50 DPA, eventually leading to the high content of C18:2n-6 in cottonseed. The content of C18:3n-3 was significantly decreased from 5 DPA (7.44%) to 25 DPA (0.11%) and correlated with the expression characteristics of Gh_A09G0848 and Gh_D09G0870. Conclusions These results contribute to our understanding on the relationship between the accumulation pattern of fatty acid components and the expression characteristics of key genes involved in fatty acid biosynthesis during the entire period of cottonseed development.

Regulation of proboscipedia in Drosophila by Homeotic Selector Genes

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 183-194
Author(s):  
Douglas B Rusch ◽  
Thomas C Kaufman
Keyword(s):  
Expression Pattern ◽  
Regulatory Element ◽  
Polycomb Group ◽  
Accumulation Pattern ◽  
Cis Acting ◽  
Polycomb Group Genes ◽  
Gap Genes ◽  
Combined Action ◽  
Selector Genes

Abstract The gene proboscipedia (pb) is a member of the Antennapedia complex in Drosophila and is required for the proper specification of the adult mouthparts. In the embryo, pb expression serves no known function despite having an accumulation pattern in the mouthpart anlagen that is conserved across several insect orders. We have identified several of the genes necessary to generate this embryonic pattern of expression. These genes can be roughly split into three categories based on their time of action during development. First, prior to the expression of pb, the gap genes are required to specify the domains where pb may be expressed. Second, the initial expression pattern of pb is controlled by the combined action of the genes Deformed (Dfd), Sex combs reduced (Scr), cap'n'collar (cnc), and teashirt (tsh). Lastly, maintenance of this expression pattern later in development is dependent on the action of a subset of the Polycomb group genes. These interactions are mediated in part through a 500-bp regulatory element in the second intron of pb. We further show that Dfd protein binds in vitro to sequences found in this fragment. This is the first clear demonstration of autonomous positive cross-regulation of one Hox gene by another in Drosophila melanogaster and the binding of Dfd to a cis-acting regulatory element indicates that this control might be direct.

scholarly journals Evaluation of micro-energy dispersive X-ray fluorescence and histochemical tests for aluminium detection in plants from High Altitude Rocky Complexes, Southeast Brazil

2014 ◽  
Vol 86 (1) ◽  
pp. 285-296 ◽  
Author(s):  
NAIARA V. CAMPOS ◽  
TIAGO A.R. PEREIRA ◽  
MARIANA F. MACHADO ◽  
MARCELO B.B. GUERRA ◽  
GLÁUCIA S. TOLENTINO ◽  
...  
Keyword(s):  
High Altitude ◽  
Accumulation Pattern ◽  
X Ray ◽  
Edx Analysis ◽  
Lycopodium Clavatum ◽  
Histochemical Tests ◽  
Ecological Success ◽  
Positive Correlations ◽  
Non Destructive ◽  
Potential Tool

The soils developed under High Altitude Rocky Complexes in Brazil are generally of very low chemical fertility, with low base saturation and high exchangeable aluminium concentration. This stressful condition imposes evolutionary pressures that lead to ecological success of plant species that are able to tolerate or accumulate high amounts of aluminium. Several analytical methods are currently available for elemental mapping of biological structures, such as micro-X-ray fluorescence (μ-EDX) and histochemical tests. The aim of this study was to combine μ-EDX analysis and histochemical tests to quantify aluminium in plants from High Altitude Rocky Complexes, identifying the main sites for Al-accumulation. Among the studied species, five showed total Al concentration higher than 1000 mg kg−1. The main Al-hyperaccumulator plants, Lavoisiera pectinata, Lycopodium clavatum and Trembleya parviflora presented positive reactions in the histochemical tests using Chrome Azurol and Aluminon. Strong positive correlations were observed between the total Al concentrations and data obtained by μ-EDX analysis. The μ-EDX analysis is a potential tool to map and quantify Al in hyperaccumulator species, and a valuable technique due to its non-destructive capacity. Histochemical tests can be helpful to indicate the accumulation pattern of samples before they are submitted for further μ-EDX scrutiny.

The Accumulation Rules of Budate Burial Hill Hydrocarbon Reservoir of Suderte Oilfield in Hailar Basin

2015 ◽  
Vol 733 ◽  
pp. 140-143
Author(s):  
Jin Hang Cai
Keyword(s):  
Source Rock ◽  
Large Scale ◽  
Metamorphic Rock ◽  
Fault System ◽  
Migration Ability ◽  
Accumulation Pattern ◽  
Hydrocarbon Reservoir ◽  
Oil Storage ◽  
Hailar Basin ◽  
Unconformity Surface

Metamorphic rock burial hill reservoir of Beier rift in Hailaer Basin, with large scale reservoir and high output has complex fault system. The fault through going direction roughly is NEE direction, and has wide fault section and lateral quickly changed fault displacement. Metamorphic rock reservoir can be divided into the vertical weathered fracture zone, crack and dissolved pores and caves development belt and tight zone. Accumulation is controlled by hydrocarbon ability of source rock, contacting relationship of source rock and reservoir, oil storage ability of reservoir, and vertical and lateral hydrocarbon migration ability of fault and unconformity surface. And formed top surface weathering crust accumulation pattern which the hydrocarbon migrated laterally along the unconformity surface, and interior reservoir pattern of crack broken zone accumulation which hydrocarbon migrated vertically along fault.

Physiological response and mineral elements accumulation pattern in Sesuvium portulacastrum L. subjected in vitro to nickel

Chemosphere ◽  
2019 ◽  
Vol 219 ◽  
pp. 463-471 ◽  
Author(s):  
Emna Fourati ◽  
Katarina Vogel-Mikuš ◽  
Taoufik Bettaieb ◽  
Anja Kavčič ◽  
Mitja Kelemen ◽  
...  
Keyword(s):  
Physiological Response ◽  
Mineral Elements ◽  
Sesuvium Portulacastrum ◽  
Accumulation Pattern

scholarly journals Comparative Genomic Analysis of TCP Genes in Six Rosaceae Species and Expression Pattern Analysis in Pyrus bretschneideri

2021 ◽  
Vol 12 ◽  
Author(s):  
Yu Zhao ◽  
Xueqiang Su ◽  
Xinya Wang ◽  
Mengna Wang ◽  
Xujing Chi ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Prunus Avium ◽  
Pattern Analysis ◽  
Evolutionary Relationship ◽  
Comparative Genomic ◽  
Accumulation Pattern ◽  
Stone Cell ◽  
Expression Pattern Analysis ◽  
Rosaceae Species ◽  
Different Tissues

TCP is a plant-specific transcription factor that plays an important role in flowering, leaf development and other physiological processes. In this study, we identified a total of 155 TCP genes: 34 in Pyrus bretschneideri, 19 in Fragaria vesca, 52 in Malus domestica, 19 in Prunus mume, 17 in Rubus occidentalis and 14 in Prunus avium. The evolutionary relationship of the TCP gene family was examined by constructing a phylogenetic tree, tracking gene duplication events, performing a sliding window analysis. The expression profile analysis and qRT-PCR results of different tissues showed that PbTCP10 were highly expressed in the flowers. These results indicated that PbTCP10 might participated in flowering induction in pear. Expression pattern analysis of different developmental stages showed that PbTCP14 and PbTCP15 were similar to the accumulation pattern of fruit lignin and the stone cell content. These two genes might participate in the thickening of the secondary wall during the formation of stone cells in pear. Subcellular localization showed that PbTCPs worked in the nucleus. This study explored the evolution of TCP genes in six Rosaceae species, and the expression pattern of TCP genes in different tissues of “Dangshan Su” pear. Candidate genes related to flower induction and stone cell formation were identified. In summary, our research provided an important theoretical basis for improving pear fruit quality and increasing fruit yield by molecular breeding.

scholarly journals Combined Transcriptome and Metabolome Integrated Analysis of Acer mandshuricum to Reveal Candidate Genes Involved in Anthocyanin Accumulation

2021 ◽  
Author(s):  
Shikai Zhang ◽  
Wang Zhan ◽  
Anran Sun ◽  
Ying Xie ◽  
Zhiming Han ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Color Change ◽  
Anthocyanin Biosynthesis ◽  
Integrated Analysis ◽  
Anthocyanin Accumulation ◽  
Structural Genes ◽  
Leaf Color ◽  
Accumulation Pattern ◽  
Color Formation ◽  
Red Color

Abstract The red color formation of Acer mandshuricum leaves is caused by the accumulation of anthocyanins primarily, but the molecular mechanism researches which underlie anthocyanin biosynthesis in A. mandshuricum were still lacking. Therefore, we combined the transcriptome and metabolome and analyzed the regulatory mechanism and accumulation pattern of anthocyanins in leaf color change periods in three different leaf color states. In our results, 26 anthocyanins were identified. Notably, the metabolite cyanidin 3-O-glucoside was found that significantly correlated with the color formation, was the predominant metabolite in anthocyanin biosynthesis of A. mandshuricum. By the way, two key structural genes ANS (Cluster-20561.86285) and BZ1 (Cluster-20561.99238) in anthocyanidin biosynthesis pathway were significantly up-regulated in RL, suggesting that they might enhance accumulation of cyanidin 3-O-glucoside which is their downstream metabolite, and contributed the red formation of A. mandshuricum leaves. Additionally, most TFs (e.g., MYBs, bZIPs and bHLHs) were detected differentially expressed in three leaf color stages that could participate in anthocyanin accumulation. This study sheds light on the anthocyanin molecular regulation of anthocyanidin bio-synthesis and accumulation underlying the different leaf color change periods in A. mandshuricum, and it could provide basic theory and new insight for the leaf color related genetic improvement of A. mandshuricum.

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