Combination of 5‐azaytidine and hanging drop culture convert fat cell into cardiac cell

2020 ◽  
Author(s):  
Zahra Bagheri‐Hosseinabadi ◽  
Fatemeh Seyedi ◽  
Hamid Reza Mollaei ◽  
Mojgan Moshrefi ◽  
Alexander Seifalian
Keyword(s):  
Cardiac Cell ◽  
Hanging Drop ◽  
Fat Cell ◽  
Hanging Drop Culture

scholarly journals Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Watchareewan Rodprasert ◽  
Sirirat Nantavisai ◽  
Koranis Pathanachai ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hanging Drop ◽  
Differentiation Potential ◽  
Insulin Producing Cells ◽  
Edmonton Protocol ◽  
Effective Generation ◽  
Hanging Drop Culture ◽  
Peptide Secretion

AbstractThe trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). The results illustrated that cBM-MSCs and cAD-MSCs contain distinct pancreatic differentiation potential and require the tailor-made induction protocols. The effective generation of cBM-MSC-derived IPCs needs the integration of genetic and microenvironment manipulation using a hanging-drop culture of PDX1-transfected cBM-MSCs under a three-step pancreatic induction protocol. However, this protocol is resource- and time-consuming. Another study on cAD-MSC-derived IPC generation found that IPC colonies could be obtained by a low attachment culture under the three-step induction protocol. Further, Notch signaling inhibition during pancreatic endoderm/progenitor induction yielded IPC colonies through the trend of glucose-responsive C-peptide secretion. Thus, this study showed that IPCs could be obtained from cBM-MSCs and cAD-MSCs through different induction techniques. Also, further signaling manipulation studies should be conducted to maximize the protocol’s efficiency.

The Roche experience with the EST assay: Development of a new algorithm and hanging drop culture device for improved prediction and full automation

2012 ◽  
Vol 34 (2) ◽  
pp. 152
Author(s):  
Claudia McGinnis ◽  
Nicole Schaefer ◽  
Tom Kissling ◽  
Thomas Zumstein ◽  
Christof Fattinger ◽  
...  
Keyword(s):  
Assay Development ◽  
Hanging Drop ◽  
Full Automation ◽  
Hanging Drop Culture

scholarly journals The formation of intestinal organoids in a hanging drop culture

2018 ◽  
Vol 70 (3) ◽  
pp. 1085-1095 ◽  
Author(s):  
Malgorzata Panek ◽  
Maja Grabacka ◽  
Malgorzata Pierzchalska
Keyword(s):  
Hanging Drop ◽  
Intestinal Organoids ◽  
Hanging Drop Culture

scholarly journals Effectiveness of mesenchymal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression

2016 ◽  
Vol 11 (1) ◽  
pp. 519-523 ◽  
Author(s):  
Yongli Lou ◽  
Dewei Guo ◽  
Hui Zhang ◽  
Laijun Song
Keyword(s):  
Gene Expression ◽  
Human Mesenchymal Stem Cells ◽  
Therapeutic Effects ◽  
Hanging Drop ◽  
Expression Levels ◽  
Conventional Culture ◽  
Damage Repair ◽  
Undifferentiated State ◽  
Stems Cells ◽  
Hanging Drop Culture

AbstractIn this study, we investigated the therapeutic effects of Human Mesenchymal Stem Cells (hMSCs) cultured by hanging drop and conventional culturing methods on cerebellar repair in hypoxic-ischemic (HI) brain injured mice. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to analyze the expression levels of three stemness genes, Oct4, Sox2 and Nanog, and the migration related gene CXCR4. MSC prepared by hanging drop or conventional techniques were administered intranasally to nine day old mice, and analyzed by MRI at day 28. Results indicate that the MSCs, especially the hanging drop cultured MSCs, significantly improved the mice’s cerebellar damage repair. MSCs derived from the hanging drop culture were smaller than those from the conventional culture. The gene expression levels were significantly increased for the MSCs derived from the hanging drop culture. The mechanism might relate to the fact that the hanging drop cultured MSCs can be kept in an undifferentiated state, resulting in its higher expression level of migration receptor of CXCR4.

Development of mouse embryos in hanging drop culture

1985 ◽  
Vol 211 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Sandra W. Potter ◽  
John E. Morris
Keyword(s):  
Mouse Embryos ◽  
Hanging Drop ◽  
Hanging Drop Culture

scholarly journals TAZ activation drives fibroblast spheroid growth, expression of profibrotic paracrine signals, and context-dependent ECM gene expression

2017 ◽  
Vol 312 (3) ◽  
pp. C277-C285 ◽  
Author(s):  
Amy J. Jorgenson ◽  
Kyoung Moo Choi ◽  
Delphine Sicard ◽  
Karry M. J. Smith ◽  
Samantha E. Hiemer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hippo Pathway ◽  
Plasminogen Activator Inhibitor ◽  
Tissue Growth ◽  
Hanging Drop ◽  
Plasminogen Activator Inhibitor 1 ◽  
Spheroid Culture ◽  
Fibroblast Activation ◽  
Context Dependent ◽  
Hanging Drop Culture

Recent studies have implicated the Hippo pathway and its transcriptional effectors YAP and TAZ as necessary for fibroblast activation and tissue fibrosis. To test the specific and sufficient roles for TAZ in driving autonomous fibroblast activation, we cultured NIH3T3 fibroblasts expressing a doxycycline-inducible nuclear-localized mutant of TAZ (TAZ4SA) in scaffold-free 3D hanging drop spheroids, or on matrices of specified mechanical rigidity. Control NIH3T3 fibroblasts formed spheroids in hanging drop culture that remained stable and neither increased nor decreased in size significantly over 15 days. In contrast, TAZ4SA-transduced fibroblasts grew robustly in spheroid culture, and expressed enhanced levels of genes encoding profibrotic soluble factors connective tissue growth factor (CTGF), endothelin-1 (Et-1), and plasminogen activator inhibitor 1 (PAI-1). However, TAZ4SA expression was unable to enhance expression of extracellular matrix (ECM)-encoding genes Col1a1, Col1a2, Col3a1, or Fn1 in spheroid culture. Micromechanical testing indicated that spheroids composed of either control or TAZ4SA-expressing cells were highly compliant and indistinguishable in mechanical properties. In fibroblasts cultured on 2D matrices of compliance similar to spheroids, TAZ4SA expression was able to enhance contractile force generation, but was unable to enhance ECM gene expression. In contrast, culture on stiff hydrogels potentiated TAZ4SA enhancement of ECM expression. TAZ4SA enhancement of Col1a1 expression on soft matrices was potentiated by TGF-β1, while on stiff matrices it was abrogated by inhibition of myocardin-related transcription factor, demonstrating context-dependent crosstalk of TAZ with these pathways. These findings demonstrate sufficiency of TAZ activation for driving fibroblast proliferation, contraction, and soluble profibrotic factor expression, and mechanical context-dependent crosstalk of TAZ with other pathways in regulating Col1a1 expression.

Hanging drop culture enhances differentiation of human adipose‐derived stem cells into anterior neuroectodermal cells using small molecules

2017 ◽  
Vol 59 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Noushin Amirpour ◽  
Shahnaz Razavi ◽  
Ebrahim Esfandiari ◽  
Batoul Hashemibeni ◽  
Mohammad Kazemi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Molecules ◽  
Adipose Derived Stem Cells ◽  
Hanging Drop ◽  
Hanging Drop Culture

A hanging drop culture method to study terminal erythroid differentiation

2005 ◽  
Vol 33 (10) ◽  
pp. 1083-1091 ◽  
Author(s):  
Laura Gutiérrez ◽  
Fokke Lindeboom ◽  
Rita Ferreira ◽  
Roy Drissen ◽  
Frank Grosveld ◽  
...  
Keyword(s):  
Culture Method ◽  
Erythroid Differentiation ◽  
Hanging Drop ◽  
Hanging Drop Culture ◽  
Terminal Erythroid Differentiation
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