Improvement of R ‐2‐(4‐hydroxyphenoxy) propionic acid biosynthesis of Beauveria bassiana by combined mutagenesis

2019 ◽  
Author(s):  
Hai‐Feng Hu ◽  
Hai‐Yan Zhou ◽  
Gao‐Ping Cheng ◽  
Ya‐Ping Xue ◽  
Yuan‐Shan Wang ◽  
...  
Keyword(s):  
Propionic Acid ◽  
Beauveria Bassiana ◽  
Acid Biosynthesis ◽  
Combined Mutagenesis

Fatty-acid biosynthesis and acetyl-coa carboxylase as a target of diclofop, fenoxaprop and other aryloxy-phenoxy-propionic acid herbicides

1988 ◽  
Vol 43 (1-2) ◽  
pp. 47-54 ◽  
Author(s):  
Klaus Kobek ◽  
Manfred Focke ◽  
K. Lichtenthaler Botanisches
Keyword(s):  
Fatty Acid ◽  
Propionic Acid ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Free Acid ◽  
Acetate Incorporation ◽  
Acetyl Coa Carboxylase ◽  
Acid Biosynthesis ◽  
Acetyl Coa ◽  
Acid Herbicides

The effect of the herbicides and aryloxy-phenoxy-propionic acid derivatives diclofop, fenoxaprop, fluazifop and haloxyfop and their ethyl, methyl or butyl esters on the de novo fatty-acid biosynthesis of isolated chloroplasts was investigated with intact chloroplasts isolated from sensitive grasses (Poaceae) and tolerant dicotyledonous plants (Pisum, Spinacia). The 4 herbicides (free-acid form) block the de novo fatty-acid biosynthesis ([2-14C]acetate incorporation into the total fatty-acid fraction) of the sensitive Avena chloroplasts in a dose-dependent manner. The I50- values (a 50% inhibition of the [14C]acetate incorporation) lie in the range of 10-7 to 2 x 10-6 ᴍ. The ethyl or methyl esters (diclofop, fenoxaprop, haloxyfop) and butyl ester (fluazifop) do not affect the de novo fatty-acid biosynthesis of isolated chloroplasts or only at a very high concentration of ca. 10-4 ᴍ. In contrast, the de novo fatty-acid biosynthesis of the tolerant dicotyledonous species (pea, spinach) is not affected by the 4 aryloxy-phenoxy-propionic acid herbicides. In an enzyme preparation isolated from chloroplasts of the herbicide-sensitive barley plants the de novo fatty-acid biosynthesis from [14C]acetate and [14C]acetyl-CoA is blocked by all 4 herbicides (free acids), whereas that of [14C]malonate and [14C]malonyl-CoA is not affected. This strongly suggests that the target of all 4 herbicides (free-acid form) is the acetyl-CoA carboxylase within the chloroplasts. The applied ester derivatives, in turn, which are ineffective in the isolated chloroplast test system, have equally little or no effect on the activity of the acetyl-CoA carboxylase. It is assumed that the acetyl-CoA carboxylase of the tolerant dicot plants investigated is modified in such a way that the 4 herbicides cannot bind to and affect the target

scholarly journals Linking genotype and phenotype in an economically viable propionic acid biosynthesis process

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Carlos H. Luna-Flores ◽  
Chris C. Stowers ◽  
Brad M. Cox ◽  
Lars K. Nielsen ◽  
Esteban Marcellin
Keyword(s):  
Propionic Acid ◽  
Acid Biosynthesis

A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis

2019 ◽  
Vol 42 (10) ◽  
pp. 1573-1582 ◽  
Author(s):  
Hai-Yan Zhou ◽  
Yi-Zuo Li ◽  
Rui Jiang ◽  
Hai-Feng Hu ◽  
Yuan-Shan Wang ◽  
...  
Keyword(s):  
Propionic Acid ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Acid Biosynthesis

Production of (R)-2-(4-hydroxyphenoxy) propionic acid by Beauveria bassiana ZJB16007 in solid state fermentation using rice bran

2020 ◽  
Vol 50 (8) ◽  
pp. 781-787
Author(s):  
Yuan-Shan Wang ◽  
Xian-Lin Wang ◽  
Hai-Yan Zhou ◽  
Hai-Feng Hu ◽  
Ya-Ping Xue ◽  
...  
Keyword(s):  
Solid State ◽  
Propionic Acid ◽  
Beauveria Bassiana ◽  
Rice Bran ◽  
Solid State Fermentation

Inhibition of early steps of de novo fatty-acid biosynthesis by different xenobiotica

1991 ◽  
Vol 81 (2) ◽  
pp. 251-255
Author(s):  
Manfred Focke ◽  
Andrea Feld ◽  
Hartmut K. Lichtenthaler
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Acid Biosynthesis

Actividad de dos hongos entomopatógenos, identificados molecularmente, sobre Bactericera cockerelli

2017 ◽  
Vol 43 (1) ◽  
pp. 27 ◽  
Author(s):  
Fabiola Villegas-Rodríguez ◽  
Ovidio Díaz-Gómez ◽  
J. Sergio Casas-Flores ◽  
Clara Teresa Monreal-Vargas ◽  
Fernando Tamayo-Mejía ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Bactericera Cockerelli ◽  
Candidatus Liberibacter

El psílido de la papa Bactericera cockerelli afecta a las solanáceas en México, por el daño directo que causa, además, de que es transmisor de patógenos procariotes como Candidatus Liberibacter solanacerum (psyllaurous). Este insecto es combatido principalmente con productos químicos. Sin embargo, en la actualidad, se buscan nuevas estrategias para su manejo como el uso de hongos entomopatógenos. El objetivo de este trabajo fue evaluar la susceptibilidad del psílido de la papa a dos cepas comerciales y dos nativas de Metarizhium anisopliae y Beauveria bassiana, así como confirmar la identificación de las mismas mediante técnicas moleculares. Los bioensayos se realizaron con la cepa nativa MA25 de M. anisopliae y las comerciales Metabich® (MA28) y Bassianil® (BB09) y la nativa BB42 de Be. bassiana. Se probaron cinco concentraciones sobre ninfas del tercer estadio de B. cockerelli y se obtuvieron sus CL50 y CL95. Todas las cepas resultaron patógenas para el psílido de la papa, las más tóxicas fueron BB09, BB42 y MA28. La mortalidad fluctuó entre 90 a 100% con las concentraciones más altas de cada cepa: la más virulenta fue BB09, con una CL50 de 2,99 x 104 conidias mL-1 y MA25 la menos virulenta tuvo una CL50 de 6,34 x 105 conidias ml-1. Mediante la clonación y la secuenciación de la región ITS de los rADN 18S, se corroboró que las cepas nativas identificadas por morfología microscópica y claves taxonómicas corresponden a Be. bassiana y M. anisopliae.

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