Metabolic flux analysis of Saccharomyces cerevisiae during redox potential–controlled very high‐gravity ethanol fermentation

2020 ◽  
Vol 67 (1) ◽  
pp. 140-147 ◽  
Author(s):  
Yishuang Zhang ◽  
Yen‐Han Lin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Redox Potential ◽  
Metabolic Flux Analysis ◽  
Ethanol Fermentation ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
High Gravity ◽  
Very High Gravity ◽  
Very High

scholarly journals Redox potential driven aeration during very-high-gravity ethanol fermentation by using flocculating yeast

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Chen-Guang Liu ◽  
Xue-Mi Hao ◽  
Yen-Han Lin ◽  
Feng-Wu Bai
Keyword(s):  
Cell Viability ◽  
Redox Potential ◽  
Ethanol Fermentation ◽  
Anaerobic Fermentation ◽  
Oxygen Demand ◽  
High Gravity ◽  
Very High Gravity ◽  
Flocculating Yeast ◽  
Aeration Process ◽  
Very High

Abstract Ethanol fermentation requires oxygen to maintain high biomass and cell viability, especially under very-high-gravity (VHG) condition. In this work, fermentation redox potential (ORP) was applied to drive the aeration process at low dissolved oxygen (DO) levels, which is infeasible to be regulated by a DO sensor. The performance and characteristics of flocculating yeast grown under 300 and 260 g glucose/L conditions were subjected to various aeration strategies including: no aeration; controlled aeration at −150, −100 and −50 mV levels; and constant aeration at 0.05 and 0.2 vvm. The results showed that anaerobic fermentation produced the least ethanol and had the highest residual glucose after 72 h of fermentation. Controlled aerations, depending on the real-time oxygen demand, led to higher cell viability than the no-aeration counterpart. Constant aeration triggered a quick biomass formation, and fast glucose utilization. However, over aeration at 0.2 vvm caused a reduction of final ethanol concentration. The controlled aeration driven by ORP under VHG conditions resulted in the best fermentation performance. Moreover, the controlled aeration could enhance yeast flocculating activity, promote an increase of flocs size, and accelerate yeast separation near the end of fermentation.

scholarly journals Selection of Saccharomyces cerevisiae strains for efficient very high gravity bio-ethanol fermentation processes

2010 ◽  
Vol 32 (11) ◽  
pp. 1655-1661 ◽  
Author(s):  
Francisco B. Pereira ◽  
Pedro M. R. Guimarães ◽  
José A. Teixeira ◽  
Lucília Domingues
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Fermentation ◽  
High Gravity ◽  
Fermentation Processes ◽  
Very High Gravity ◽  
Very High ◽  
Selection Of
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