Cloning and bacterial expression systems for recombinant human heparanase production: Substrate specificity investigation by docking of a putative heparanase substrate

2017 ◽  
Vol 65 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Angela Pennacchio ◽  
Alessandro Capo ◽  
Simonetta Caira ◽  
Annabella Tramice ◽  
Antonio Varriale ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems

Comparative Analysis of Bacterial Expression Systems for Keratinase Production

2014 ◽  
Vol 173 (5) ◽  
pp. 1222-1235 ◽  
Author(s):  
Baihong Liu ◽  
Juan Zhang ◽  
Lei Gu ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems

Generation of antibodies towards MASP-1 and MASP-2 using bacterial expression systems

1998 ◽  
Vol 35 (6-7) ◽  
pp. 409 ◽  
Author(s):  
Steen Vang Petersen ◽  
K. Poulsen ◽  
C.M. Stover ◽  
C. Koch ◽  
T. Vorup-Jensen ◽  
...  
Keyword(s):  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems

scholarly journals Positively regulated bacterial expression systems

2008 ◽  
Vol 2 (1) ◽  
pp. 15-30 ◽  
Author(s):  
Trygve Brautaset ◽  
Rahmi Lale ◽  
Svein Valla
Keyword(s):  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems

scholarly journals A Review on Molecular Functional and Structural Characterization of Human Cysteine Cathepsins in Bacterial Expression Systems

2019 ◽  
Author(s):  
Ruwini Cooray ◽  
Hasanka Madubashetha ◽  
Lakshan Warnakula ◽  
P.D.S.U. Wickramasinghe ◽  
Nimali De Silva
Keyword(s):  
Structural Characterization ◽  
Cancer Metastasis ◽  
Expression System ◽  
Bacterial Expression ◽  
Immune Functions ◽  
Expression Systems ◽  
Biochemical Pathways ◽  
Cysteine Cathepsins ◽  
Bacterial Expression Systems

Cysteine cathepsins, a class of proteinaceous enzymes, regulate a wide variety of metabolic processes in human including protein breakdown and turnover and immune functions. Eleven cysteine cathepsins have been identified so far and a wide array of studies related to identifying their specific functions, regulation and distribution patterns in tissues have been conducted. However, in recent past, the association of cysteine cathepsins in occurrence and progression of cancers have been identified and this has caused unrest in scientists triggering them to investigate the physiology, biochemical pathways and interactions of these cathepsins in cancer metastasis and therefore has become a noteworthy topic of intensive research. This review focusses and collects together the published work on molecular functional and structural characterization studies that have been done so far on in vitro expression of genes encoding for cysteine cathepsins in the Escherichia coli bacterial expression system. Accordingly, it was found out that all cathepsins except for cathepsins K, C, H, X and W have been expressed this way and the majority of them were found to be expressed in E. coli BL21(DE3) pLysS expression host via pET3 expression vector. In addition, it was also noted that in most of the expression studies, the substrate that was used to validate the enzymatic activity of the recombinant enzyme that was produced was a cysteine residue along with a benzyloxy-carbonyl salt. Through this review, the authors suggest that there is a very high need that all cysteine cathepsins need to be characterized both structurally and functionally on a molecular platform to better understand their interactions including the biochemical pathways. It is also momentous that the mass production of the recombinant forms of these enzymes are facilitated via expression in such bacterial expression systems and in turn, would also provide a strong platform for the development and progression of studies related to human physiology including oncological studies such as cancer metastasis. Moreover, as per biochemical features of the enzymes that could be identified, the production of efficient inhibitors or inducers as per the necessity to improve health and promote wellbeing among the mankind could be facilitated.

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