Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity

2015 ◽  
Vol 64 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Pavel V. Panteleev ◽  
Tatiana V. Ovchinnikova
Keyword(s):  
Antimicrobial Peptide ◽  
High Cell ◽  
Recombinant Production ◽  
Cell Selectivity ◽  
Tachyplesin I

scholarly journals Bee venom-derived antimicrobial peptide melectin has broad-spectrum potency, cell selectivity, and salt-resistant properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Su Jin Ko ◽  
Eunji Park ◽  
Alina Asandei ◽  
Jee-Young Choi ◽  
Seung-Chul Lee ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Broad Spectrum ◽  
Bee Venom ◽  
Cell Selectivity

scholarly journals Experimental Induction of Bacterial Resistance to the Antimicrobial Peptide Tachyplesin I and Investigation of the Resistance Mechanisms

2016 ◽  
Vol 60 (10) ◽  
pp. 6067-6075 ◽  
Author(s):  
Jun Hong ◽  
Jianye Hu ◽  
Fei Ke
Keyword(s):  
Antimicrobial Peptide ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Antibacterial Drug ◽  
Cationic Antimicrobial Peptide ◽  
Content Type ◽  
Tachyplesin I

ABSTRACTTachyplesin I is a 17-amino-acid cationic antimicrobial peptide (AMP) with a typical cyclic antiparallel β-sheet structure that is a promising therapeutic for infections, tumors, and viruses. To date, no bacterial resistance to tachyplesin I has been reported. To explore the safety of tachyplesin I as an antibacterial drug for wide clinical application, we experimentally induced bacterial resistance to tachyplesin I by using two selection procedures and studied the preliminary resistance mechanisms.Aeromonas hydrophilaXS91-4-1,Pseudomonas aeruginosaCGMCC1.2620, andEscherichia coliATCC 25922 and F41 showed resistance to tachyplesin I under long-term selection pressure with continuously increasing concentrations of tachyplesin I. In addition,P. aeruginosaandE. coliexhibited resistance to tachyplesin I under UV mutagenesis selection conditions. Cell growth and colony morphology were slightly different between control strains and strains with induced resistance. Cross-resistance to tachyplesin I and antimicrobial agents (cefoperazone and amikacin) or other AMPs (pexiganan, tachyplesin III, and polyphemusin I) was observed in some resistant mutants. Previous studies showed that extracellular protease-mediated degradation of AMPs induced bacterial resistance to AMPs. Our results indicated that the resistance mechanism ofP. aeruginosawas not entirely dependent on extracellular proteolytic degradation of tachyplesin I; however, tachyplesin I could induce increased proteolytic activity inP. aeruginosa. Most importantly, our findings raise serious concerns about the long-term risks associated with the development and clinical use of tachyplesin I.

Recombinant Production and Intein-Mediated Purification of an Antimicrobial Peptide, BR2

2017 ◽  
Vol 23 (4) ◽  
pp. 501-507 ◽  
Author(s):  
Fatemeh Shafiee ◽  
Ghazale Minaiyan ◽  
Fatemeh Moazen ◽  
Ali Jahanian-Najafabadi
Keyword(s):  
Antimicrobial Peptide ◽  
Recombinant Production

scholarly journals Cell selectivity correlates with membrane-specific interactions: A case study on the antimicrobial peptide G15 derived from granulysin

2006 ◽  
Vol 1758 (2) ◽  
pp. 154-163 ◽  
Author(s):  
Ayyalusamy Ramamoorthy ◽  
Sathiah Thennarasu ◽  
Anmin Tan ◽  
Dong-Kuk Lee ◽  
Carol Clayberger ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Specific Interactions ◽  
Cell Selectivity

scholarly journals 3P024 Analysis of chitin binding ability of an antimicrobial peptide tachyplesin I derived from horseshoe crab(01A. Protein: Structure,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S215
Author(s):  
Takahiro Kushibiki ◽  
Masakatsu Kamiya ◽  
Tomoyasu Aizawa ◽  
Yasuhiro Kumiki ◽  
Takashi Kikukawa ◽  
...  
Keyword(s):  
Protein Structure ◽  
Antimicrobial Peptide ◽  
Horseshoe Crab ◽  
Binding Ability ◽  
Tachyplesin I

Design, Synthesis and Analysis of a Cysteine-Deleted Analog of the Antimicrobial Peptide Tachyplesin-I

2010 ◽  
pp. 269-270
Author(s):  
Deborah L. Heyl ◽  
Kiran Kumar Gottipati ◽  
Sreeja Sreekumar ◽  
Sathan Thennarasu ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
Antimicrobial Peptide ◽  
Design Synthesis ◽  
Tachyplesin I
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