Phenol removal from refinery wastewater by mutant recombinant horseradish peroxidase

2014 ◽  
Vol 61 (2) ◽  
pp. 226-229 ◽  
Author(s):  
Sedigheh Asad ◽  
Bahareh Dabirmanesh ◽  
Khosro Khajeh
Keyword(s):  
Horseradish Peroxidase ◽  
Phenol Removal ◽  
Refinery Wastewater ◽  
Recombinant Horseradish Peroxidase

Peroxidase-catalyzed removal of phenols from a petroleum refinery wastewater

2001 ◽  
Vol 43 (2) ◽  
pp. 253-260 ◽  
Author(s):  
M. Wagner ◽  
J. A. Nicell
Keyword(s):  
Horseradish Peroxidase ◽  
Petroleum Refinery ◽  
Phenol Removal ◽  
Refinery Wastewater ◽  
Phenol Content ◽  
Petroleum Refinery Wastewater

The phenol content of a petroleum refinery wastewater was reduced below the discharge limit following treatment with horseradish peroxidase and H2O2. Approximately 58% of COD, 78% of BOD5, and 95% of toxicity were removed along with the phenols. As a result of treatment, phenols were transformed into less biodegradable compounds which could be removed by subsequent coagulation and precipitation. Optimization of the peroxide concentration led to 20% enzyme savings. The use of PEG and chitosan as protective additives resulted in 4 and 25-fold reductions in enzyme requirements, respectively. Phenol removal did not appear to be adversely affected by the presence of other hydrocarbons that are frequently present in refinery wastewaters.

Expression of recombinant horseradish peroxidase C in Escherichia coli

1989 ◽  
Vol 17 (6) ◽  
pp. 1077-1078 ◽  
Author(s):  
JULIAN F. BURKE ◽  
ANDREW SMITH ◽  
NIOVI SANTAMA ◽  
ROBERT C. BRAY ◽  
ROGER N. F. THORNELEY ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Horseradish Peroxidase ◽  
Recombinant Horseradish Peroxidase

Characterization of Recombinant Horseradish Peroxidase C and three Site-Directed mutants, F41V, F41W, and R38K by Resonance Raman Spectroscopy

Biochemistry ◽  
1994 ◽  
Vol 33 (23) ◽  
pp. 7398-7407 ◽  
Author(s):  
Giulietta Smulevich ◽  
Mauro Paoli ◽  
Julian F. Burke ◽  
Andrew T. Smith ◽  
Stephen A. Sanders ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Horseradish Peroxidase ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Recombinant Horseradish Peroxidase

scholarly journals Application of Response Surface Methodology to Enhance Phenol Removal from Refinery Wastewater by Microwave Process

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Sherif A. Younis ◽  
Waleed I. El-Azab ◽  
Nour Sh. El-Gendy ◽  
Shuokr Qarani Aziz ◽  
Yasser M. Moustafa ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Output Power ◽  
Petroleum Refinery ◽  
Irradiation Time ◽  
Reaction Rates ◽  
Design Parameters ◽  
Phenol Removal ◽  
Refinery Wastewater ◽  
Petroleum Refinery Wastewater

Phenol contaminated petroleum refinery wastewater presents a great threat on water resources safety. This study investigates the effect of microwave irradiation on removal of different concentrations of phenol in an attempt for petroleum refinery wastewater treatment. The obtained results show that the MW output power and irradiation time have a significant positive effect on the removal efficiency of phenol. The kinetic reaction is significantly affected by initial MW output power and initial phenol concentrations. Response surface methodology (RSM) was employed to optimize and study the interaction effects of process parameters: MW output power, irradiation time, salinity, pH, and H2O2 concentration using central composite design (CCD). From the CCD design matrix, a quadratic model was considered as an ultimate model (R2 = 0.75) and its adequacy was justified through analysis of variance (ANOVA). The overall reaction rates were significantly enhanced in the combined MW/H2O2 system as proved by RSM. The optimum values for the design parameters of the MW/H2O2 process were evaluated giving predicted phenol removal percentage of 72.90% through RSM by differential approximation and were confirmed by experimental phenol removal of 75.70% in a batch experiment at optimum conditions of 439 W MW power, irradiation time of 24.22 min, salinity of 574 mg/L, pH 5.10, and initial H2O2 concentration of 10% (v/v).

Studies on the Refolding Process of Recombinant Horseradish Peroxidase

2012 ◽  
Vol 54 (2) ◽  
pp. 484-492 ◽  
Author(s):  
Sedigheh Asad ◽  
Bahareh Dabirmanesh ◽  
Nasser Ghaemi ◽  
Seyed Masoud Etezad ◽  
Khosro Khajeh
Keyword(s):  
Horseradish Peroxidase ◽  
Recombinant Horseradish Peroxidase
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