Expression and characterization of Rv0447c product, potentially the methyltransferase involved in tuberculostearic acid biosynthesis inMycobacterium tuberculosis

2013 ◽  
Vol 60 (4) ◽  
pp. 412-416 ◽  
Author(s):  
Laxman S. Meena ◽  
Pappachan E. Kolattukudy
Keyword(s):  
Acid Biosynthesis ◽  
Tuberculostearic Acid

Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51

Biofouling ◽  
2017 ◽  
Vol 34 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Xiaochun Huang ◽  
Chang Chen ◽  
Chunhua Ren ◽  
Yingying Li ◽  
Yiqin Deng ◽  
...  
Keyword(s):  
Vibrio Alginolyticus ◽  
Acid Biosynthesis ◽  
Colanic Acid ◽  
Identification And Characterization

scholarly journals Characterization of Ligand Binding to the Bifunctional Key Enzyme in the Sialic Acid Biosynthesis by NMR

2004 ◽  
Vol 279 (53) ◽  
pp. 55722-55727 ◽  
Author(s):  
Andrew J. Benie ◽  
Astrid Blume ◽  
Richard R. Schmidt ◽  
Werner Reutter ◽  
Stephan Hinderlich ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Ligand Binding ◽  
Acid Biosynthesis ◽  
Key Enzyme

scholarly journals Isolation and Characterization of FecA- and FeoB-Mediated Iron Acquisition Systems of the Spirochete Leptospira biflexa by Random Insertional Mutagenesis

2005 ◽  
Vol 187 (9) ◽  
pp. 3249-3254 ◽  
Author(s):  
Hélène Louvel ◽  
Isabelle Saint Girons ◽  
Mathieu Picardeau
Keyword(s):  
Insertional Mutagenesis ◽  
Iron Acquisition ◽  
Transposon Mutagenesis ◽  
Ecological Niches ◽  
Acid Biosynthesis ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Transposon Mutants

ABSTRACT The specific mechanisms by which Leptospira spp. acquire iron from their ecological niches are unknown. A major factor contributing to our ignorance of spirochetal biology is the lack of methods for genetic analysis of these organisms. In this study, we have developed a system for random transposon mutagenesis of Leptospira biflexa using a mariner transposon, Himar1. To demonstrate the validity of Himar1 in vivo transposon mutagenesis in L. biflexa, a screen of mutants for clones impaired in amino acid biosynthesis was first performed, enabling the identification of tryptophan and glutamate auxotrophs. To investigate iron transporters, 2,000 L. biflexa transposon mutants were screened onto media with and without hemin, thus allowing the identification of five hemin-requiring mutants, and the putative genes responsible for this phenotype were identified. Three mutants had distinct insertions in a gene encoding a protein which shares homology with the TonB-dependent receptor FecA, involved in ferric citrate transport. We also identified two mutants with a Himar1 insertion into a feoB-like gene, the product of which is required for ferrous iron uptake in many bacterial organisms. Interestingly, the growth inhibition exhibited by the fecA and feoB mutants was relieved by deferoxamine, suggesting the presence of a ferric hydroxamate transporter. These results confirm the importance of iron for the growth of Leptospira and its ability to use multiple iron sources.

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