Large-scale production of human mesenchymal stem cells for clinical applications

2012 ◽  
Vol 59 (2) ◽  
pp. 106-120 ◽  
Author(s):  
Sunghoon Jung ◽  
Krishna M. Panchalingam ◽  
Reynold D. Wuerth ◽  
Lawrence Rosenberg ◽  
Leo A. Behie
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Human Mesenchymal Stem Cells ◽  
Clinical Applications ◽  
Scale Production ◽  
Large Scale Production

From “Bench to Bedside”: Current Advancement on Large-Scale Production of Mesenchymal Stem Cells

2017 ◽  
Vol 26 (22) ◽  
pp. 1662-1673 ◽  
Author(s):  
Varitsara Bunpetch ◽  
Haoyu Wu ◽  
Shufang Zhang ◽  
Hongwei Ouyang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Large‐Scale Production of LGR5‐Positive Bipotential Human Liver Stem Cells

Hepatology ◽  
2020 ◽  
Vol 72 (1) ◽  
pp. 257-270 ◽  
Author(s):  
Kerstin Schneeberger ◽  
Natalia Sánchez‐Romero ◽  
Shicheng Ye ◽  
Frank G. Steenbeek ◽  
Loes A. Oosterhoff ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Liver ◽  
Large Scale ◽  
Scale Production ◽  
Liver Stem Cells ◽  
Large Scale Production

Large-Scale Production of Human Adipose Tissue from Stem Cells: A New Tool for Regenerative Medicine and Tissue Banking

2008 ◽  
pp. 110306231138043
Author(s):  
Francesco D'andrea ◽  
Francesco De Francesco ◽  
Giuseppe A. Ferraro ◽  
Vincenzo Desiderio ◽  
Virginia Tirino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Tissue Banking ◽  
Human Adipose Tissue ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Large-Scale Expansion of Human Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Muhammad Najib Fathi Bin Hassan ◽  
Muhammad Dain Yazid ◽  
Mohd Heikal Mohd Yunus ◽  
Shiplu Roy Chowdhury ◽  
Yogeswaran Lokanathan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Human Mesenchymal Stem Cells ◽  
Expansion Ratio ◽  
Spinner Flask ◽  
Roller Bottle ◽  
Multipotent Stem Cells ◽  
Cardiovascular Damage ◽  
Scale Expansion

Mesenchymal stem cells (MSCs) are multipotent stem cells with strong immunosuppressive property that renders them an attractive source of cells for cell therapy. MSCs have been studied in multiple clinical trials to treat liver diseases, peripheral nerve damage, graft-versus-host disease, autoimmune diseases, diabetes mellitus, and cardiovascular damage. Millions to hundred millions of MSCs are required per patient depending on the disease, route of administration, frequency of administration, and patient body weight. Multiple large-scale cell expansion strategies have been described in the literature to fetch the cell quantity required for the therapy. In this review, bioprocessing strategies for large-scale expansion of MSCs were systematically reviewed and discussed. The literature search in Medline and Scopus databases identified 26 articles that met the inclusion criteria and were included in this review. These articles described the large-scale expansion of 7 different sources of MSCs using 4 different bioprocessing strategies, i.e., bioreactor, spinner flask, roller bottle, and multilayered flask. The bioreactor, spinner flask, and multilayered flask were more commonly used to upscale the MSCs compared to the roller bottle. Generally, a higher expansion ratio was achieved with the bioreactor and multilayered flask. Importantly, regardless of the bioprocessing strategies, the expanded MSCs were able to maintain its phenotype and potency. In summary, the bioreactor, spinner flask, roller bottle, and multilayered flask can be used for large-scale expansion of MSCs without compromising the cell quality.

scholarly journals Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Satoshi Fujita ◽  
Harue Shimizu ◽  
Shin-ichiro Suye
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Clinical Applications ◽  
Soluble Factors ◽  
Precise Control ◽  
Mixed Medium ◽  
Aligned Nanofibers ◽  
Different Types ◽  
Msc Differentiation

Effective differentiation of mesenchymal stem cells (MSCs) is required for clinical applications. To control MSC differentiation, induction media containing different types of soluble factors have been used to date; however, it remains challenging to obtain a uniformly differentiated population of an appropriate quality for clinical application by this approach. We attempted to develop nanofiber scaffolds for effective MSC differentiation by mimicking anisotropy of the extracellular matrix structure, to assess whether differentiation of these cells can be controlled by using geometrically different scaffolds. We evaluated MSC differentiation on aligned and random nanofibers, fabricated by electrospinning. We found that induction of MSCs into adipocytes was markedly more inhibited on random nanofibers than on aligned nanofibers. In addition, adipoinduction on aligned nanofibers was also inhibited in the presence of mixed adipoinduction and osteoinduction medium, although osteoinduction was not affected by a change in scaffold geometry. Thus, we have achieved localized control over the direction of differentiation through changes in the alignment of the scaffold even in the presence of a mixed medium. These findings indicate that precise control of MSC differentiation can be attained by using scaffolds with different geometry, rather than by the conventional use of soluble factors in the medium.

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