Gold Nanorod-Photosensitizer Complex Obtained by Layer-by-Layer Method for Photodynamic/Photothermal Therapy In Vitro

2015 ◽  
Vol 10 (3) ◽  
pp. 563-567 ◽  
Author(s):  
Seung Beom Kim ◽  
Tae Heon Lee ◽  
Il Yoon ◽  
Young Key Shim ◽  
Woo Kyoung Lee
Keyword(s):  
Photothermal Therapy ◽  
Gold Nanorod ◽  
Layer By Layer ◽  
Layer Method

scholarly journals The Most Effective Gold Nanorod Size for Plasmonic Photothermal Therapy: Theory and In Vitro Experiments

2014 ◽  
Vol 118 (5) ◽  
pp. 1319-1326 ◽  
Author(s):  
Megan A. Mackey ◽  
Moustafa R. K. Ali ◽  
Lauren A. Austin ◽  
Rachel D. Near ◽  
Mostafa A. El-Sayed
Keyword(s):  
Photothermal Therapy ◽  
Gold Nanorod ◽  
In Vitro Experiments ◽  
Plasmonic Photothermal Therapy

Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System

2005 ◽  
Vol 288-289 ◽  
pp. 101-104 ◽  
Author(s):  
Ke Feng Ren ◽  
Jian Ji ◽  
Jia Cong Shen
Keyword(s):  
Self Assembly ◽  
Multilayer Films ◽  
Layer By Layer ◽  
The Novel ◽  
Gene Delivery System ◽  
Layer Method ◽  
Uv Visible ◽  
Spectrometry Measurement ◽  
Visible Spectrometry

Films composed of alternating layers of protamine and DNA were constructed using the layer-by-layer method on quartz and subsequently studied the enzymic degradation in vitro. UV-visible spectrometry measurement indicated the uniform assembly of Protamine/DNA multilayer films. UV-visible spectrometry and fluorescence spectrometry results revealed that the Protamine/DNA multilayer films were in vitro enzymic biodegradable. The novel biodegradable multilayer of Protamine/DNA may have great potential for gene therapy applications in tissue engineering, medical implant etc.

Gene Therapy and Photothermal Therapy of Layer-by-Layer Assembled AuNCs /PEI/miRNA/ HA Nanocomplexes

2019 ◽  
Vol 19 (4) ◽  
pp. 330-337 ◽  
Author(s):  
Li-Juan Yan ◽  
Xin-Hong Guo ◽  
Wei-Ping Wang ◽  
Yu-Rong Hu ◽  
Shao-Feng Duan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Photothermal Therapy ◽  
Antitumor Effect ◽  
Average Particle Size ◽  
Uv Spectra ◽  
Hydrodynamic Diameter ◽  
Target Cells ◽  
Average Particle ◽  
Layer By Layer

Background:MicroRNA (miRNA) therapy, which was widely considered to treat a series of cancer, has been confronted with numerous obstacles to being delivered into target cells because of its easy biodegradation and instability.Methods:In this research, we successfully constructed 11-mercaptoundecanoic acid modified gold nanocages (AuNCs)/polyethyleneimine (PEI)/miRNA/hyaluronic acid (HA) complexes (abbreviated as AuNCs/PEI/miRNA/HA) using a layer-by-layer method for target-specific intracellular delivery of miRNA by HA receptor mediated endocytosis.Results:The results of UV spectra, hydrodynamic diameter and zeta potential analyses confirmed the formation of AuNCs/PEI/ miRNA/HA complex with its average particle size of ca. 153 nm and surface charge of ca. -9.43 mV. Next, we evaluated the antitumor effect of the nanocomplex mediated by the combination of gene therapy and photothermal therapy (PTT) against hepatocellular carcinoma (HCC) in vitro.Conclusion:Our experimental results indicated that the AuNCs/PEI/miRNA/HA complex effectively delivered miRNA to the target cells and its antitumor effect was significantly enhanced by the combination of gene therapy and photothermal therapy. In addition, anti-miR-181b could promote Bel-7402 cell arrest in S phase and improve TIMP-3 mRNA expression. All these results suggested that AuNCs/PEI/miRNA/HA gene delivery system with combination of gene therapy and photothermal therapy might be exploited for HCC treatment.

scholarly journals Hollow gold nanoshells-incorporated injectable genetically engineered hydrogel for sustained chemo-photothermal therapy of tumor

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
RuiMei Jin ◽  
Jie Yang ◽  
DongHui Zhao ◽  
XiaoLin Hou ◽  
ChaoQing Li ◽  
...  
Keyword(s):  
Photothermal Therapy ◽  
Combined Therapy ◽  
Genetically Engineered ◽  
Gold Nanoshells ◽  
Photothermal Effect ◽  
Layer By Layer ◽  
Hollow Gold Nanoshells ◽  
New Strategy

Abstract Background Combined therapy has demonstrated to be an effective strategy for cancer therapy. Herein, an injectable hydrogel based on the genetically engineered polypeptide and hollow gold nanoshells (HAuNS) has been developed for chemo-photothermal therapy of HepG2 tumor. Methods PC10A/DOX/HAuNS nanogel was prepared with layer-by-layer through the adsorption of DOX and PC10A successively. DOX with positive charge and PC10A with negative charge were coated step by step onto the surface of negatively charged HAuNS. The multifunctional hydrogel PC10A/DOX/HAuNS were prepared via dissolving hybrid PC10A/DOX/HAuNS nanogel in polypeptide PC10A. Chemotherapy drug DOX in the PC10A/DOX/HAuNS hydrogel was absorbed on the HAuNS and directly embedded in the PC10A hydrogel, which contributes to sequentially release of the drug. Specifically, DOX adsorbed on the HAuNS could be released slowly for sustainable chemotherapy. Results The PC10A/DOX/HAuNS hydrogel could pass 26-gauge needle without clogging, indicating that it is injectable. In addition, the PC10A/DOX/HAuNS hydrogel possessed outstanding photothermal effect and photothermal stability. In both in vitro cell and in vivo tumor-bearing mice experiments, a remarkably enhance tumor inhibition was observed by the combined therapy of chemo-photothermal therapy compared with photothermal therapy or chemotherapy alone. Conclusions The combined chemotherapy and photothermal therapy of PC10A/DOX/HAuNS hydrogels could significantly improve the therapeutic effect. Therefore, the multifunctional hydrogel PC10A/DOX/HAuNS is promising to provide a new strategy for sustained chemo-photothermal therapy.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

scholarly journals Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 261
Author(s):  
Wei Mao ◽  
Sol Lee ◽  
Ji Un Shin ◽  
Hyuk Sang Yoo
Keyword(s):  
Gold Nanoparticles ◽  
Transfection Efficiency ◽  
Cancer Therapeutics ◽  
Atom Transfer ◽  
Layer By Layer ◽  
Dependent Manner ◽  
Metallic Particles ◽  
Anti Cancer

Surface initiated atom transfer radical polymerization (SI-ATRP) documented a simple but efficient technique to grow a dense polymer layer on any surface. Gold nanoparticles (AuNPs) give a broad surface to immobilize sulfhyryl group-containing initiators for SI-ATRP; in addition, AuNPs are the major nanoparticulate carriers for delivery of anti-cancer therapeutics, since they are biocompatible and bioinert. In this work, AuNPs with a disulfide initiator were polymerized with sulfoethyl methacrylate by SI-ATRP to decorate the particles with anionic corona, and branched polyethyeleneimine (PEI) and siRNA were sequentially layered onto the anionic corona of AuNP by electrostatic interaction. The in vitro anti-cancer effect confirmed that AuNP with anionic corona showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study of tumor-bearing nude mice revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size in comparison to those with free siRNA for 4 weeks. Furthermore, histological examination and gene expression study revealed that the decorated AuNP significantly suppressed c-Myc expression. Thus, we envision that the layer-by-layer assembly on the anionic brushes can be potentially used to incorporate nucleic acids onto metallic particles with high transfection efficiency.

scholarly journals Fabrication of cyanine dye thin films grown by a layer-by-layer method

2015 ◽  
Vol 2 (7) ◽  
pp. 076402 ◽  
Author(s):  
Junpei Nagauchi ◽  
Osamu Kojima ◽  
Takashi Kita ◽  
YongGu Shim
Keyword(s):  
Thin Films ◽  
Cyanine Dye ◽  
Layer By Layer ◽  
Layer Method
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