Two‐Photon Probes for Zn 2+ Ions with Various Dissociation Constants. Detection of Zn 2+ Ions in Live Cells and Tissues by Two‐Photon Microscopy

2011 ◽  
Vol 6 (5) ◽  
pp. 1234-1240 ◽  
Author(s):  
Isravel Antony Danish ◽  
Chang Su Lim ◽  
Yu Shun Tian ◽  
Ji Hee Han ◽  
Min Young Kang ◽  
...  
Keyword(s):  
Dissociation Constants ◽  
Live Cells ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Volumetric two-photon imaging in live cells and embryos via axially gradient excitation

2019 ◽  
Author(s):  
Yufeng Gao ◽  
Xianyuan Xia ◽  
Jia Yu ◽  
Tingai Chen ◽  
Zhili Xu ◽  
...  
Keyword(s):  
Live Cells ◽  
Deep Penetration ◽  
Layer By Layer ◽  
Photon Beams ◽  
Volumetric Imaging ◽  
Localization Accuracy ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Subcellular Resolution ◽  
Imaging Speed

AbstractTwo-photon microscopy(TPM) that features subcellular resolution, intrinsic optical sectioning ability, and deep penetration in sample is a powerful tool of bioimaging. However, the process of layer-by-layer scanning to form a 3D image inherently limits the volumetric imaging speed and significantly increases the phototoxicity. Here we develop a gradient TPM technique that enables rapid volumetric imaging by only acquiring two 2D images. By sequentially exciting the specimen with two axially elongated two-photon beams with complementary gradient intensities, the axial positions of fluorophores can be decoded from the intensity ratio of the paired images. We achieve an axial localization accuracy of 0.728 ± 0.657 μm, which is sufficient for rapid 3D subcellular imaging. We demonstrate the flexibility of the gradient TPM on a variety of sparsely labelled samples, including bead phantoms, mouse brain tissues, live macrophages and live nematode embryos. The results show that, compared with conventional TPM, the 3D imaging speed increases 6 folds while the photobleaching and photodamage are extremely reduced.

scholarly journals Molecular Tattooing of Live Cells and Animals: Spatial and Time Specific Effects of Azidoblebbistatin by Two-Photon Microscopy

2014 ◽  
Vol 106 (2) ◽  
pp. 177a
Author(s):  
Miklos Kepiro ◽  
Boglarka Varkuti ◽  
Gyorgy Hegyi ◽  
Miklos S.Z. Kellermayer ◽  
Malnasi-Csizmadia Andras
Keyword(s):  
Live Cells ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Specific Effects ◽  
Time Specific

scholarly journals A FRET Based Two-Photon Fluorescent Probe for Visualizing Mitochondrial Thiols of Living Cells and Tissues

Sensors ◽  
2020 ◽  
Vol 20 (6) ◽  
pp. 1746 ◽  
Author(s):  
Zhengkun Liu ◽  
Qianqian Wang ◽  
Hao Wang ◽  
Wenting Su ◽  
Shouliang Dong
Keyword(s):  
High Selectivity ◽  
Limit Of Detection ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fast Response ◽  
Live Cells ◽  
Biological Processes ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Main Component

Glutathione (GSH) is the main component of the mitochondrial thiol pool and plays key roles in the biological processes. Many evidences have suggested that cysteine and homocysteine also exist in mitochondria and are interrelated with GSH in biological systems. The fluctuation of the levels of mitochondrial thiols has been linked to many diseases and cells’ dysfunction. Therefore, the monitoring of mitochondrial thiol status is of great significance for clinical studies. We report here a novel fluorescence resonance energy transfer based two-photon probe MT-1 for mitochondrial thiols detection. MT-1 was constructed by integrating the naphthalimide moiety (donor) and rhodamine B (accepter and targeting group) through a newly designed linker. MT-1 shows a fast response, high selectivity, and sensitivity to thiols, as well as a low limit of detection. The two-photon property of MT-1 allows the direct visualization of thiols in live cells and tissues by two-photon microscopy. MT-1 can serve as an effective tool to unravel the diverse biological functions of mitochondrial thiols in living systems.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

ACS Omega ◽  
2020 ◽  
Author(s):  
Kazushi Yamaguchi ◽  
Kohei Otomo ◽  
Yuichi Kozawa ◽  
Motosuke Tsutsumi ◽  
Tomoko Inose ◽  
...  
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vincent D. Ching-Roa ◽  
Eben M. Olson ◽  
Sherrif F. Ibrahim ◽  
Richard Torres ◽  
Michael G. Giacomelli
Keyword(s):  
Dynamic Range ◽  
High Dynamic Range ◽  
Silicon Photomultipliers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
High Dynamic

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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