scholarly journals Transforming growth factor β controls CCN3 expression in nucleus pulposus cells of the intervertebral disc

2011 ◽  
Vol 63 (10) ◽  
pp. 3022-3031 ◽  
Author(s):  
Cassie M. Tran ◽  
Harvey E. Smith ◽  
Aviva Symes ◽  
Laure Rittié ◽  
Bernard Perbal ◽  
...  
Keyword(s):  
Growth Factor ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Nucleus Pulposus Cells ◽  
Ccn3 Expression

scholarly journals Transforming Growth Factor β Enhances Tissue Formation by Passaged Nucleus Pulposus Cells In Vitro

2019 ◽  
Vol 38 (2) ◽  
pp. 438-449 ◽  
Author(s):  
Sajjad Ashraf ◽  
Kenny Chatoor ◽  
Jasmine Chong ◽  
Robert Pilliar ◽  
Paul Santerre ◽  
...  
Keyword(s):  
Growth Factor ◽  
Nucleus Pulposus ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Tissue Formation ◽  
Nucleus Pulposus Cells

scholarly journals EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs

2021 ◽  
Vol 11 (9) ◽  
pp. 4072
Author(s):  
Benjamin Gantenbein ◽  
Rahel D. May ◽  
Paola Bermudez-Lekerika ◽  
Katharina A. C. Oswald ◽  
Lorin M. Benneker ◽  
...  
Keyword(s):  
Growth Factor ◽  
Nucleus Pulposus ◽  
Transforming Growth Factor ◽  
Diffuse Idiopathic Skeletal Hyperostosis ◽  
Transforming Growth Factor Β ◽  
Intervertebral Discs ◽  
Serum Marker ◽  
Ectopic Ossification ◽  
Disc Cells ◽  
Bmp Pathway

Diffuse idiopathic skeletal hyperostosis (DISH) is characterised by ectopic ossification along the anterior spine and the outer intervertebral discs (IVD). However, the centre of the IVD, i.e., the nucleus pulposus, always remains unaffected, which could be due to the inhibition of the bone morphogenetic protein (BMP) pathway. In this study, we investigated the transcriptome for the BMP pathway of DISH-IVD cells versus disc cells of traumatic or degenerative discs. The disc cells originated from nucleus pulposus (NP), annulus fibrosus (AF) and from cartilaginous endplate (CEP) tissue. Here, ninety genes of the transforming growth factor β-BMP signalling pathway were screened by qPCR. Furthermore, the protein expression of genes of interest was further investigated by immune-staining and semi-quantitative microscopy. IVDs of three DISH patients were tested against three control patients (same disc level and similar age). Early Growth Response 2 (EGR2) and Interleukin 6 (IL6) were both significantly up-regulated in DISH-IVD cells compared to controls (12.8 ± 7.6-fold and 54.0 ± 46.5-fold, respectively, means ± SEM). Furthermore, Insulin-like Growth Factor 1 (IGF1) tended to be up-regulated in DISH-IVD donors, i.e., 174.13 ± 120.6-fold. IGF1 was already known as a serum marker for DISH and other rheumatoid diseases and is confirmed here to play a possible key role in DISH-IVD.

scholarly journals Ligustrazine Prevents Intervertebral Disc Degeneration via Suppression of Aberrant TGFβ Activation in Nucleus Pulposus Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Shufen Liu ◽  
Yuhao Cheng ◽  
Yuqi Tan ◽  
Jingcheng Dong ◽  
Qin Bian
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Ex Vivo ◽  
Tissue Growth ◽  
Nucleus Pulposus Cells

Objectives. Aberrant transforming growth factor β (TGFβ) activation is detrimental to both nucleus pulposus (NP) cells and cartilage endplates (CEPs), which can lead to intervertebral disc degeneration (IDD). Ligustrazine (LIG) reduces the expression of inflammatory factors and TGFβ1 in hypertrophic CEP to prevent IDD. In this study, we investigate the effects of LIG on NP cells and the TGFβ signaling. Design. LIG was injected to the lumbar spinal instability (LSI) mouse model. The effect of LIG was evaluated by intervertebral disc (IVD) score in the LSI mouse model. The expression of activated TGFβ was examined using immunostaining with pSmad2/3 antibody. The upright posture (UP) rat model was also treated and evaluated in the same manner to assess the effect of LIG. In ex vivo study, IVDs from four-week old mice were isolated and treated with 10−5, 10−6, and 10−7 M of LIG. We used western blot to detect activated TGFβ expression. TGFβ-treated human nucleus pulposus cells (HNPCs) were cotreated with optimized dose of LIG in vitro. Immunofluorescence staining was performed to determine pSmad2/3, connective tissue growth factor (CCN2), and aggrecan (ACAN) expression levels. Results. IVD score and the percentage of pSmad2/3+ NP cells were low in LIG-treated LSI mice in comparison with LSI mice, but close to the levels in the Sham group. Similarly, LIG reduced the overexpression of TGFβ1 in NP cells. The inhibitory effect of LIG was dose dependent. A dose of 10−5 M LIG not only strongly attenuated Smad2/3 phosphorylation in TGFβ-treated IVD ex vivo but also suppressed pSmad2/3, CCN2, and ACAN expression in TGFβ-treated NP cells in vitro. Conclusions. LIG prevents IDD via suppression of TGFβ overactivation in NP cells.

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