scholarly journals Visualization and in situ analysis of leukocyte trafficking into the ankle joint in a systemic murine model of rheumatoid arthritis

2005 ◽  
Vol 52 (10) ◽  
pp. 3269-3278 ◽  
Author(s):  
István Gál ◽  
Éva Bajnok ◽  
Sándor Szántó ◽  
Bara Sarraj ◽  
Tibor T. Glant ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankle Joint ◽  
Murine Model ◽  
In Situ Analysis ◽  
Leukocyte Trafficking

In Situ Analysis and Visualization with CREATE-AVTMHelios

2021 ◽  
Author(s):  
Andrew C. Bauer ◽  
James R. Forsythe ◽  
Jay Sitaraman ◽  
Andrew M. Wissink ◽  
Buvaneswari Jayaraman ◽  
...  
Keyword(s):  
In Situ Analysis

scholarly journals Identification of potential autoantigens in anti-CCP-positive and anti-CCP-negative rheumatoid arthritis using citrulline-specific protein arrays

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Thomas B. G. Poulsen ◽  
Dres Damgaard ◽  
Malene M. Jørgensen ◽  
Ladislav Senolt ◽  
Jonathan M. Blackburn ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Protein Microarray ◽  
Specific Protein ◽  
Immune Reactivity ◽  
Igg Antibodies ◽  
Native Proteins ◽  
Array Technology ◽  
Citrullinated Proteins ◽  
Folded Proteins

AbstractThe presence or absence of autoantibodies against citrullinated proteins (ACPAs) distinguishes two main groups of rheumatoid arthritis (RA) patients with different etiologies, prognoses, disease severities, and, presumably, disease pathogenesis. The heterogeneous responses of RA patients to various biologics, even among ACPA-positive patients, emphasize the need for further stratification of the patients. We used high-density protein array technology for fingerprinting of ACPA reactivity. Identification of the proteome recognized by ACPAs may be a step to stratify RA patients according to immune reactivity. Pooled plasma samples from 10 anti-CCP-negative and 15 anti-CCP-positive RA patients were assessed for ACPA content using a modified protein microarray containing 1631 different natively folded proteins citrullinated in situ by protein arginine deiminases (PADs) 2 and PAD4. IgG antibodies from anti-CCP-positive RA plasma showed high-intensity binding to 87 proteins citrullinated by PAD2 and 99 proteins citrullinated by PAD4 without binding significantly to the corresponding native proteins. Curiously, the binding of IgG antibodies in anti-CCP-negative plasma was also enhanced by PAD2- and PAD4-mediated citrullination of 29 and 26 proteins, respectively. For only four proteins, significantly more ACPA binding occurred after citrullination with PAD2 compared to citrullination with PAD4, while the opposite was true for one protein. We demonstrate that PAD2 and PAD4 are equally efficient in generating citrullinated autoantigens recognized by ACPAs. Patterns of proteins recognized by ACPAs may serve as a future diagnostic tool for further subtyping of RA patients.

scholarly journals Refilling of cortical calcium stores in Paramecium cells: in situ analysis in correlation with store-operated calcium influx

Cell Calcium ◽  
2003 ◽  
Vol 34 (1) ◽  
pp. 87-96 ◽  
Author(s):  
I. Mohamed ◽  
M. Husser ◽  
I. Sehring ◽  
J. Hentschel ◽  
C. Hentschel ◽  
...  
Keyword(s):  
Calcium Influx ◽  
In Situ Analysis ◽  
Calcium Stores
Sign in / Sign up

Export Citation Format

Share Document