scholarly journals Effects of combination M40403 and dexamethasone therapy on joint disease in a rat model of collagen-induced arthritis

2005 ◽  
Vol 52 (6) ◽  
pp. 1929-1940 ◽  
Author(s):  
Salvatore Cuzzocrea ◽  
Emanuela Mazzon ◽  
Rosanna Di Paola ◽  
Tiziana Genovese ◽  
Carmelo Muià ◽  
...  
Keyword(s):  
Rat Model ◽  
Joint Disease ◽  
Collagen Induced Arthritis ◽  
Dexamethasone Therapy

Amelioration of joint disease in a rat model of collagen-induced arthritis by M40403, a superoxide dismutase mimetic

2001 ◽  
Vol 44 (12) ◽  
pp. 2909-2921 ◽  
Author(s):  
Daniela Salvemini ◽  
Emanuela Mazzon ◽  
Laura Dugo ◽  
Ivana Serraino ◽  
Angela De Sarro ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Rat Model ◽  
Joint Disease ◽  
Collagen Induced Arthritis

scholarly journals Treatment of collagen-induced arthritis rat model by using Notch signalling inhibitor

2021 ◽  
Vol 28 ◽  
pp. 100-107
Author(s):  
Jianhai Chen ◽  
Jian Li ◽  
Jinqing Chen ◽  
Wenxiang Cheng ◽  
Jietao Lin ◽  
...  
Keyword(s):  
Rat Model ◽  
Notch Signalling ◽  
Collagen Induced Arthritis

scholarly journals Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Fengfan Yang ◽  
Xing Luo ◽  
Wenxiao Zhu ◽  
Jia Li ◽  
Zhaohui Zheng ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Active Rheumatoid Arthritis ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Immunofluorescence Staining ◽  
Joint Disease ◽  
Collagen Induced Arthritis ◽  
Das28 Score ◽  
Immune Pathways ◽  
Positive Correlations

Innate lymphoid cells (ILCs) have roles in many diseases and immune pathways. To determine the roles of these cells in patients with rheumatoid arthritis (RA) and mice with collagen-induced arthritis (CIA), we measured ILC subsets using flow cytometry and multiplex immunofluorescence staining. Patients with stable RA had greater proportions of ILC2s and decreased proportions of ILC1s and ILC3s (all p < 0.05 ). The 28-joint disease activity (DAS28) score had positive correlations with the proportion of ILC1s and negative correlations with ILC2s (both p < 0.05 ). ILC2s of patients with RA expressed more IL-4 than healthy controls ( p < 0.05 ). The proportions of ILC1s and ILC2s were greater in mice with CIA (both p < 0.05 ), especially the ILC2s in mice without arthritis after immunization and had correlations with multiple inflammatory and anti-inflammatory cytokines. Multiplex immunofluorescence staining described the distribution of ILCs in spleen tissues. Our results indicate that dysregulation of ILCs occurs during the pathogenesis of RA and CIA.

Discovery of Serum Proteomic Biomarkers for Prediction of Response to Moxibustion Treatment in Rats with Collagen-Induced Arthritis: An Exploratory Analysis

2016 ◽  
Vol 34 (3) ◽  
pp. 184-193 ◽  
Author(s):  
Xiao Xu ◽  
Miao-Miao Wang ◽  
Zhi-ling Sun ◽  
Dan-ping Zhou ◽  
Ling Wang ◽  
...  
Keyword(s):  
Rat Model ◽  
Multiple Testing ◽  
Day Treatment ◽  
Expression Patterns ◽  
Control Group ◽  
Sprague Dawley ◽  
Collagen Induced Arthritis ◽  
Serum Samples ◽  
Beneficial Effects ◽  
Bovine Collagen

Objective To examine the possible impact of moxibustion on the serum proteome of the collagen-induced arthritis (CIA) rat model. Materials and Methods Thirty-six male Sprague-Dawley rats were included in this experiment. The CIA animal model was prepared by injection of type II bovine collagen in Freund's adjuvant on the first and seventh day. The 36 rats were randomly divided into two groups: the untreated CIA group (control), and the CIA plus treatment with moxibustion (CIA+moxi) group. Moxibustion was administered daily at ST36 and BL23 for 7, 14 or 21 days (n=12 rats each). Arthritis score was used to assess the severity of arthritis. At the end of each 7 day treatment, blood samples from the control group and the CIA+moxi group were collected. After removal of high abundance proteins from serum samples, two-dimensional gel combined with matrix-assisted laser desorption ionisation time-of-flight MS/MS (MALDI-TOF-MS/MS) techniques were performed to examine serum protein expression patterns of the CIA rat model with and without moxibustion treatment. In addition, the relevant proteins were further analysed with the use of bioinformatics analysis. Results Moxibustion significantly decreased arthritis severity in the rats in the CIA+moxi group, when compared with the rats in the CIA group 35 days after the first immunisation (p=0.001). Seventeen protein spots which changed >1.33 or <0.77 at p<0.05 using Bonferonni correction for multiple testing were found to be common to all three comparisons, and these proteins were used for classification of functions using the Gene Ontology method. Consequently, with the use of the Ingenuity Pathway Analysis, the top canonical pathways and a predicted proteomic network related to the moxibustion effect of CIA were established. Conclusions Using the proteomics technique, we have identified novel candidate proteins that may be involved in the mechanisms of action underlying the beneficial effects of moxibustion in rats with CIA. Our findings suggest that immune responses and metabolic processes may be involved in mediating the effects of moxibustion. Moreover, periodxiredoxin I (PRDX1) and inositol 1,4,5-triphosphate receptor (IP3R) may be potential targets.

scholarly journals Therapeutic Efficacy of Vitamin Eδ-Tocotrienol in Collagen-Induced Rat Model of Arthritis

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nagaraja Haleagrahara ◽  
Mirashini Swaminathan ◽  
Srikumar Chakravarthi ◽  
Ammu Radhakrishnan
Keyword(s):  
Rheumatoid Arthritis ◽  
Rat Model ◽  
Therapeutic Efficacy ◽  
Intradermal Injection ◽  
Histopathological Analysis ◽  
Paw Edema ◽  
Collagen Induced Arthritis ◽  
Reactive Protein ◽  
Significant Reversal ◽  
Systemic Inflammatory Disease

Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory disease primarily involving inflammation of the joints. Although the management of the disease has advanced significantly in the past three decades, there is still no cure for RA. The aim of this study was to determine the therapeutic efficacy ofδ-tocotrienol, in the rat model of collagen-induced arthritis (CIA). Arthritis was induced by intradermal injection of collagen type II emulsified in complete Freund’s adjuvant. CIA rats were orally treated withδ-tocotrienol (10 mg/kg) or glucosamine hydrochloride (300 mg/kg) from day 25 to 50. Efficacy was assessed based on the ability to reduce paw edema, histopathological changes, suppression of collagen-specific T-cells, and a reduction in C-reactive protein (CRP) levels. It was established thatδ-tocotrienol had the most significant impact in lowering paw edema when compared to glucosamine treatment. Paw edema changes correlated well with histopathological analysis where there was a significant reversal of changes in groups treated withδ-tocotrienol. The results suggest thatδ-tocotrienol is efficient in amelioration of collagen-induced arthritis. Vitamin E delta-tocotrienol may be of therapeutic value against rheumatoid arthritis.

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