scholarly journals Small interfering RNA inhibition of SPARC attenuates the profibrotic effect of transforming growth factor ?1 in cultured normal human fibroblasts

2005 ◽  
Vol 52 (1) ◽  
pp. 257-261 ◽  
Author(s):  
Xiaodong Zhou ◽  
Filemon K. Tan ◽  
Xinjian Guo ◽  
Debra Wallis ◽  
Dianna M. Milewicz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Small Interfering Rna ◽  
Transforming Growth Factor ◽  
Human Fibroblasts ◽  
Normal Human ◽  
Rna Inhibition ◽  
Interfering Rna

scholarly journals Single small-interfering RNA expression vector for silencing multiple transforming growth factor-  pathway components

2005 ◽  
Vol 33 (15) ◽  
pp. e131-e131 ◽  
Author(s):  
A. Jazag ◽  
F. Kanai ◽  
H. Ijichi ◽  
K. Tateishi ◽  
T. Ikenoue ◽  
...  
Keyword(s):  
Growth Factor ◽  
Small Interfering Rna ◽  
Expression Vector ◽  
Transforming Growth Factor ◽  
Rna Expression ◽  
Interfering Rna

scholarly journals Interaction between Smad7 and β-Catenin: Importance for Transforming Growth Factor β-Induced Apoptosis

2005 ◽  
Vol 25 (4) ◽  
pp. 1475-1488 ◽  
Author(s):  
Sofia Edlund ◽  
So Young Lee ◽  
Susanne Grimsby ◽  
Shouthing Zhang ◽  
Pontus Aspenström ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Fate ◽  
Small Interfering Rna ◽  
Transforming Growth Factor ◽  
Glycogen Synthase ◽  
Transforming Growth Factor Β ◽  
Specific Factor ◽  
Dependent Manner ◽  
Induced Apoptosis ◽  
Interfering Rna

ABSTRACT Members of the transforming growth factor β (TGF-β) and Wnt/wingless superfamilies regulate cell fate during development and tissue maintenance. Here we report that Smad7 interacts with β-catenin and lymphoid enhancer binding factor 1/T-cell-specific factor (LEF1/TCF), transcriptional regulators in Wnt signaling, in a TGF-β-dependent manner. Smad7 was found to be required for TGF-β1-induced accumulation of β-catenin and LEF1 in human prostate cancer (PC-3U) cells as well as in human keratinocytes (HaCaT cells). Moreover, when the endogenous Smad7 was repressed by specific small interfering RNA, TGF-β-induced increase of activated p38, Akt phosphorylated on Ser473, glycogen synthase kinase 3β phosphorylated on Ser9 was prevented, as well as the TGF-β-induced association between β-catenin and LEF1. Notably, the observed physical association of Smad7 and β-catenin was found to be important for TGF-β-induced apoptosis, since suppression of β-catenin expression by small interfering RNA decreased the apoptotic response to TGF-β.

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