Freshly isolated osteoarthritic chondrocytes are catabolically more active than normal chondrocytes, but less responsive to catabolic stimulation with interleukin-1?

2005 ◽  
Vol 52 (1) ◽  
pp. 136-143 ◽  
Author(s):  
Zhiyong Fan ◽  
Brigitte Bau ◽  
Huiqing Yang ◽  
Stephan Soeder ◽  
Thomas Aigner
Keyword(s):  
Interleukin 1 ◽  
Normal Chondrocytes ◽  
Osteoarthritic Chondrocytes

Expression of the VEGF receptor-3 in osteoarthritic chondrocytes: stimulation by interleukin-1? and association with ?1-integrins

2003 ◽  
Vol 120 (3) ◽  
pp. 235-241 ◽  
Author(s):  
M. Shakibaei ◽  
G. Schulze-Tanzil ◽  
A. Mobasheri ◽  
T. Beichler ◽  
J. Dressler ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Vegf Receptor ◽  
Osteoarthritic Chondrocytes

Platelet-Rich Plasma Releasate Inhibits Inflammatory Processes in Osteoarthritic Chondrocytes

2011 ◽  
Vol 39 (11) ◽  
pp. 2362-2370 ◽  
Author(s):  
Gerben M. van Buul ◽  
Wendy L.M. Koevoet ◽  
Nicole Kops ◽  
P. Koen Bos ◽  
Jan A.N. Verhaar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Matrix Metalloproteinase ◽  
Platelet Rich Plasma ◽  
Interleukin 1 ◽  
Cartilage Degeneration ◽  
No Production ◽  
Major Pathway ◽  
Mediated Effects ◽  
Osteoarthritic Chondrocytes ◽  
A Disintegrin And Metalloproteinase

Background: Platelet-rich plasma (PRP) has recently been postulated as a treatment for osteoarthritis (OA). Although anabolic effects of PRP on chondrocytes are well documented, no reports are known addressing effects on cartilage degeneration. Since OA is characterized by a catabolic and inflammatory joint environment, the authors investigated whether PRP was able to counteract the effects of such an environment on human osteoarthritic chondrocytes. Hypothesis: Platelet-rich plasma inhibits inflammatory effects of interleukin-1 (IL-1) beta on human osteoarthritic chondrocytes. Study Design: Controlled laboratory study. Methods: Human osteoarthritic chondrocytes were cultured in the presence of IL-1 beta to mimic an osteoarthritic environment. Medium was supplemented with 0%, 1%, or 10% PRP releasate (PRPr, the active releasate of PRP). After 48 hours, gene expression of collagen type II alpha 1 (COL2A1), aggrecan (ACAN), a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)4, ADAMTS5, matrix metalloproteinase (MMP)13, and prostaglandin-endoperoxide synthase (PTGS)2 was analyzed. Additionally, glycosaminoglycan (GAG) content, nitric oxide (NO) production, and nuclear factor kappa B (NFκB) activation were studied. Results: Platelet-rich plasma releasate diminished IL-1 beta–induced inhibition of COL2A1 and ACAN gene expression. The PRPr also reduced IL-1 beta–induced increase of ADAMTS4 and PTGS2 gene expression. ADAMTS5 gene expression and GAG content were not influenced by IL-1 beta or additional PRPr. Matrix metalloproteinase 13 gene expression and NO production were upregulated by IL-1 beta but not affected by added PRPr. Finally, PRPr reduced IL-1 beta–induced NFκB activation to control levels containing no IL-1 beta. Conclusion: Platelet-rich plasma releasate diminished multiple inflammatory IL-1 beta–mediated effects on human osteoarthritic chondrocytes, including inhibition of NFκB activation. Clinical Relevance: Platelet-rich plasma releasate counteracts effects of an inflammatory environment on genes regulating matrix degradation and formation in human chondrocytes. Platelet-rich plasma releasate decreases NFκB activation, a major pathway involved in the pathogenesis of OA. These results encourage further study of PRP as a treatment for OA.

scholarly journals The procatabolic cytokine interleukin-1 alfa induces a dysregulation of o-linked n-acetylglucosamine modified proteins in human osteoarthritic chondrocytes

2014 ◽  
Vol 22 ◽  
pp. S171-S172
Author(s):  
A. Larrañaga-Vera ◽  
J. Andres-Bergos ◽  
S. Perez-Baos ◽  
G. Herrero-Beaumont ◽  
R. Largo
Keyword(s):  
Interleukin 1 ◽  
Osteoarthritic Chondrocytes ◽  
Modified Proteins
Sign in / Sign up

Export Citation Format

Share Document