Low-dose gold compounds inhibit fibroblast proliferation and do not affect interleukin-1 secretion by macrophages

1988 ◽  
Vol 31 (10) ◽  
pp. 1272-1280 ◽  
Author(s):  
Tsukasa Matsubara ◽  
Yasuhiro Saegusa ◽  
Kazushi Hirohata
Keyword(s):  
Low Dose ◽  
Interleukin 1 ◽  
Fibroblast Proliferation ◽  
Gold Compounds

scholarly journals Auranofin-Mediated NRF2 Induction Attenuates Interleukin 1 Beta Expression in Alveolar Macrophages

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 632
Author(s):  
Stephanie B. Wall ◽  
Rui Li ◽  
Brittany Butler ◽  
Ashley R. Burg ◽  
Hubert M. Tse ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Inflammatory Responses ◽  
Interleukin 1 ◽  
Direct Interaction ◽  
Synthesis Methods ◽  
Related Factor ◽  
Gold Compound ◽  
Nuclear Factor ◽  
Glutathione Synthesis ◽  
Gold Compounds

Background: Alveolar macrophages (AMs) are resident inflammatory cells in the lung that serve as early sentinels of infection or injury. We have identified thioredoxin reductase 1 inhibition by gold compounds increases activation of nuclear factor erythroid 2-related factor 2 (NRF2)-dependent pathways to attenuate inflammatory responses. The present studies utilized murine alveolar macrophages (MH-S) to test the hypothesis that the gold compound, auranofin (AFN), decreases interleukin (IL)-1β expression through NRF2-mediated interactions with nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway genes and/or increases in glutathione synthesis. Methods: MH-S cells were treated with AFN and lipopolysaccharide (LPS) and analyzed at 6 and 24 h. The Il1b promoter was analyzed by chromatin immunoprecipitation for direct interaction with NRF2. Results: Expression of IL-1β, p-IκBα, p-p65 NF-kB, and NOD-, LRR-, and pyrin domain-containing protein 3 were elevated by LPS exposure, but only IL-1β expression was suppressed by AFN treatment. Both AFN and LPS treatments increased cellular glutathione levels, but attenuation of glutathione synthesis by buthionine sulfoximine (BSO) did not alter expression of Il-1β. Analysis revealed direct NRF2 binding to the Il1b promoter which was enhanced by AFN and inhibited the transcriptional activity of DNA polymerase II. Conclusions: Our data demonstrate that AFN-induced NRF2 activation directly suppresses IL-1β synthesis independent of NFκB and glutathione-mediated antioxidant mechanisms. NRF2 binding to the promoter region of IL1β directly inhibits transcription of the IL1β gene. Collectively, our research suggests that gold compounds elicit NRF2-dependent pulmonary protection by suppressing macrophage-mediated inflammation.

scholarly journals Possible Protective Effect of Diacerein on Doxorubicin-Induced Nephrotoxicity in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Marwa M. M. Refaie ◽  
Entesar F. Amin ◽  
Nashwa F. El-Tahawy ◽  
Aly M. Abdelrahman
Keyword(s):  
Protective Effect ◽  
Low Dose ◽  
Caspase 3 ◽  
Interleukin 1 ◽  
Treated Group ◽  
Limiting Factors ◽  
High Dose ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

Nephrotoxicity is one of the limiting factors for using doxorubicin (DOX). Interleukin 1 has major role in DOX-induced nephrotoxicity, so we investigated the effect of interleukin 1 receptor antagonist diacerein (DIA) on DOX-induced nephrotoxicity. DIA (25 and 50 mg/kg/day) was administered orally to rats for 15 days, in the presence or absence of nephrotoxicity induced by a single intraperitoneal injection of DOX (15 mg/kg) at the 11th day. We measured levels of serum urea, creatinine, renal reduced glutathione (GSH), malondialdehyde (MDA), total nitrites (NOx), catalase, and superoxide dismutase (SOD). In addition, caspase-3, tumor necrosis factor alpha (TNFα), nuclear factor kappa B (NFκB) expressions, and renal histopathology were assessed. Our results showed that DOX-induced nephrotoxicity was ameliorated or reduced by both doses of DIA, but diacerein high dose (DHD) showed more improvement than diacerein low dose (DLD). This protective effect was manifested by significant improvement in all measured parameters compared to DOX treated group by using DHD. DLD showed significant improvement of creatinine, MDA, NOx, GSH, histopathology, and immunohistochemical parameters compared to DOX treated group.

scholarly journals Characterization of a monoclonal antibody directed against the biologically active site of human interleukin 1.

1986 ◽  
Vol 163 (2) ◽  
pp. 463-468 ◽  
Author(s):  
A Köck ◽  
M Danner ◽  
B M Stadler ◽  
T A Luger
Keyword(s):  
Monoclonal Antibody ◽  
Active Site ◽  
Biological Effects ◽  
Interleukin 1 ◽  
Biologically Active ◽  
Size Exclusion ◽  
Fibroblast Proliferation ◽  
Initial Activity ◽  
Exclusion Chromatography

Human IL-1 was successfully used to produce an anti-IL-1 mAb. Anti-IL-1 (IgG2a) blocked IL-1-mediated thymocyte and fibroblast proliferation, but did not interfere with the biological effects of other lymphokines, such as IL-2 or IL-3. The antibody immunoprecipitated biosynthetically radiolabeled 33, 17, and 4 kD IL-1. An immunoadsorbent column yielded 20% of initial activity, and upon HPLC size-exclusion chromatography, affinity-purified IL-1 had a molecular mass of approximately 4 kD. These results provide first evidence of a monoclonal anti-IL-1 that reacts with different species of IL-1 and apparently binds to an epitope close to the active site of IL-1. Thus, anti-IL-1 IgG may be very helpful for further investigations of the molecular as well as biological characteristics of IL-1 and related mediators.

scholarly journals Induction of fibroblast proliferation by interleukin-1 derived from human monocytic leukemia cells

1984 ◽  
Vol 27 (9) ◽  
pp. 995-1001 ◽  
Author(s):  
Arnold E. Postlethwaite ◽  
Lawrence B. Lachman ◽  
Andrew H. Kang
Keyword(s):  
Interleukin 1 ◽  
Leukemia Cells ◽  
Fibroblast Proliferation ◽  
Monocytic Leukemia ◽  
Human Monocytic Leukemia

scholarly journals A low dose of recombinant interleukin 1 protects granulocytopenic mice from lethal gram-negative infection.

1988 ◽  
Vol 85 (5) ◽  
pp. 1620-1623 ◽  
Author(s):  
J. W. van der Meer ◽  
M. Barza ◽  
S. M. Wolff ◽  
C. A. Dinarello
Keyword(s):  
Low Dose ◽  
Interleukin 1 ◽  
Gram Negative
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