scholarly journals Estimation of the relative avidity of 19S IgM rheumatoid factor secreted by rheumatoid synovial cells for human IgG subclasses

1986 ◽  
Vol 29 (6) ◽  
pp. 722-729 ◽  
Author(s):  
Dick L. Robbins ◽  
Jeffrey Skilling ◽  
William F. Benisek ◽  
Richard Wistar
Keyword(s):  
Rheumatoid Factor ◽  
Igg Subclasses ◽  
Synovial Cells ◽  
Human Igg ◽  
Igm Rheumatoid Factor

scholarly journals Human immunoglobulin (IgG) induced deletion of IgM rheumatoid factor B cells in transgenic mice.

1995 ◽  
Vol 181 (2) ◽  
pp. 599-606 ◽  
Author(s):  
H Tighe ◽  
P Heaphy ◽  
S Baird ◽  
W O Weigle ◽  
D A Carson
Keyword(s):  
B Cells ◽  
Transgenic Mice ◽  
B Cell ◽  
Rheumatoid Factor ◽  
Immunoglobulin Gene ◽  
Protein Antigens ◽  
Human Igg ◽  
Variable Regions ◽  
Igm Rheumatoid Factor ◽  
Induction Of Tolerance

The singular ability of immunoglobulin genes to hypermutate their variable regions, while permitting the generation of high-affinity antibodies against foreign antigens, poses a problem in terms of maintenance of immunological self-tolerance. Immunoglobulin gene hypermutation driven by a foreign antigen has the potential to generate antibodies that cross-react with self-components. Consequently, there must exist a mechanism in the periphery for inactivation of mature autoreactive B cell clones. The classical experimental system used to address this problem is the induction of tolerance to soluble, deaggregated human IgG. We have analyzed the mechanism of induction of tolerance to human IgG using transgenic mice that express a human IgM rheumatoid factor (IgM RF) on a large proportion of their B cells. Injection of deaggregated human IgG caused a specific deletion of those B cells that express an intact IgM RF on their cell surface. The degree of RF B cell deletion was proportional to the reduction in the proliferative response of splenocytes to antigen (aggregated human IgG), or to F(ab')2 fragments of anti-human IgM antibodies. Control experiments showed that IgG administration had little effect on the numbers of mouse Ig-bearing cells or their ability to proliferate to a nonspecific mitogen. Thus, the effects of IgG on the human IgM RF B cell are antigen specific and are not due to nonspecific toxic effects of the human IgG preparation. These experiments demonstrate that peripheral exposure to IgG induces deletion of reactive B cells, without any evidence for anergy, and differ from data obtained by other investigators studying tolerance to soluble protein antigens. The results imply that human Igs have distinct properties as soluble antigens, and that peripheral nonresponsiveness to IgG may be due to lymphocyte deletion.

Turbidimetry of rheumatoid factor in serum with a centrifugal analyzer.

1986 ◽  
Vol 32 (1) ◽  
pp. 124-129 ◽  
Author(s):  
L Borque ◽  
M Yago ◽  
C Mar ◽  
C Rodriguez
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Serum ◽  
Rheumatoid Factor ◽  
Test Results ◽  
Serum Samples ◽  
Human Igg ◽  
Standard Curve ◽  
Igm Rheumatoid Factor ◽  
Triglyceride Concentration ◽  
Reference Serum

Abstract We describe the simple, rapid turbidimetry of IgM rheumatoid factor in human serum by use of the Cobas-Bio centrifugal analyzer. Heat-aggregated human IgG is used as the antigen. The immunoturbidimetric reaction is monitored at 340 nm for 300 s, and the changes in absorbance after the antigen is added are used to prepare the standard curve. Test results are calculated from the stored curve and reported in int. units/mL, based on comparison with the WHO reference serum for rheumatoid factor. There is no interference from bilirubin (up to 340 mumol/L) or hemoglobin (up to 5600 mg/L). Serum samples with a triglyceride concentration greater than 2.20 mmol/L must be cleared of lipids before analysis. The standard curve is linear from 30 to 500 int. units/mL. Precision, accuracy, linearity, and sensitivity are quite acceptable. The CV was generally less than 5% for different concentrations of rheumatoid factor. Results agree well with those by a rate-nephelometric procedure on the Beckman ICS system (rs = 0.932). However, both correlate poorly with a modified classical Waaler-Rose test. Of 47 patients with rheumatoid arthritis, 34 had IgM rheumatoid factor in their serum, but the measured value did not reflect the activity of the disease.

scholarly journals IgA rheumatoid factor synthesis by dissociated synovial cells. Characterization and relationship to IgM rheumatoid factor synthesis

1985 ◽  
Vol 28 (11) ◽  
pp. 1219-1227 ◽  
Author(s):  
William J. Koopman ◽  
Ralph E. Schrohenloher ◽  
Sylvia S. Crago ◽  
David M. Spalding ◽  
Jiri Mestecky
Keyword(s):  
Rheumatoid Factor ◽  
Synovial Cells ◽  
Igm Rheumatoid Factor

Specific cleavage sites on human IgG subclasses by cruzipain, the major cysteine proteinase from Trypanosoma cruzi

2003 ◽  
Vol 130 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Patricia Berasain ◽  
Carlos Carmona ◽  
Blas Frangione ◽  
Juan José Cazzulo ◽  
Fernando Goñi
Keyword(s):  
Trypanosoma Cruzi ◽  
Cysteine Proteinase ◽  
Igg Subclasses ◽  
Cleavage Sites ◽  
Human Igg

Cleavage of the Four Human IgG Subclasses with Cathepsin G

1982 ◽  
Vol 16 (6) ◽  
pp. 487-498 ◽  
Author(s):  
A. BAICI ◽  
M. KNOPFEL ◽  
K. FEHR
Keyword(s):  
Igg Subclasses ◽  
Cathepsin G ◽  
Human Igg
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