scholarly journals Ciprofloxacin enhances the stimulation of matrix metalloproteinase 3 expression by interleukin-1? in human tendon-derived cells

2002 ◽  
Vol 46 (11) ◽  
pp. 3034-3040 ◽  
Author(s):  
Anthony N. Corps ◽  
Rebecca L. Harrall ◽  
Valerie A. Curry ◽  
Steven A. Fenwick ◽  
Brian L. Hazleman ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Interleukin 1 ◽  
Matrix Metalloproteinase 3 ◽  
Human Tendon ◽  
Stimulation Of

scholarly journals Interleukin-1β-induced matrix metalloproteinase-3 via ERK1/2 pathway to promote mesenchymal stem cell migration

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0252163
Author(s):  
Chun-Hao Chang ◽  
Yun-Li Lin ◽  
Yeu-Sheng Tyan ◽  
Yun-Hsuan Chiu ◽  
Ya-Han Liang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Matrix Metalloproteinase ◽  
Neural Progenitor Cells ◽  
Interleukin 1 ◽  
Migration And Invasion ◽  
Human Umbilical Cord ◽  
Akt Inhibitor ◽  
P38 Inhibitor ◽  
Matrix Metalloproteinase 3 ◽  
Well Assay

Human umbilical cord Wharton’s jelly derived mesenchymal stem cells (hUCMSCs), a source of cell therapy, have received a great deal of attention due to their homing or migrating ability in response to signals emanating from damaged sites. It has been found that IL-1β possesses the ability to induce the expression of matrix metalloproteinase-3 (MMP-3) in bone marrow MSCs. MMP-3 is involved in cell migration in various types of cells, including glioblastoma, vascular smooth muscle, and adult neural progenitor cells. In this study, we proposed that IL-1β influences hUCMSCs migration involving MMP-3. The expression level of MMP-3 in IL-1β-induced hUCMSCs was verified using cDNA microarray analysis, quantitative real-time PCR, ELISA and Western blot. Wound-healing and trans-well assay were used to investigate the cell migration and invasion ability of IL-1β-treated hUCMSCs. In addition, we pre-treated hUCMSCs with interleukin-1 receptor antagonist, MMP-3 inhibitors (ALX-260-165, UK 356618), or transfected with MMP-3 siRNA to confirm the role of MMP3 in IL-1β-induced cell migration. Our results showed that IL-1β induced MMP-3 expression is related to the migration of hUCMSCs. Moreover, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) inhibitor U0126, p38 inhibitor SB205380, JNK inhibitor SP600125 and Akt inhibitor GSK 690693 decreased IL-1β-induced MMP-3 mRNA and protein expression. The migration and invasion ability analyses showed that these inhibitors attenuated the IL-1β-induced migration and invasion ability of hUCMSCs. In conclusion, we have found that IL-1β induces the expression of MMP-3 through ERK1/2, JNK, p38 MAPK and Akt signaling pathways to enhance the migration of hUCMSCs. These results provide further understanding of the mechanisms in IL-1β-induced hUCMSCs migration to injury sites.

scholarly journals 5-Aza-2′-deoxycytidine and interleukin-1 cooperate to regulate matrix metalloproteinase-3 gene expression

2011 ◽  
Vol 129 (9) ◽  
pp. 2083-2092 ◽  
Author(s):  
Julie Couillard ◽  
Pierre-Olivier Estève ◽  
Sriharsa Pradhan ◽  
Yves St-Pierre
Keyword(s):  
Gene Expression ◽  
Matrix Metalloproteinase ◽  
Interleukin 1 ◽  
Matrix Metalloproteinase 3

Activin A suppresses interleukin-1-induced matrix metalloproteinase 3 secretion in human chondrosarcoma cells

2007 ◽  
Vol 27 (11) ◽  
pp. 1049-1055 ◽  
Author(s):  
Deh-Ming Chang ◽  
Shao-Hsiang Liu ◽  
Herng-Sheng Lee ◽  
Jenn-Hung Lai ◽  
Chen-Hung Chen
Keyword(s):  
Matrix Metalloproteinase ◽  
Interleukin 1 ◽  
Activin A ◽  
Matrix Metalloproteinase 3 ◽  
Human Chondrosarcoma

scholarly journals The 45 kDa collagen-binding fragment of fibronectin induces matrix metalloproteinase-13 synthesis by chondrocytes and aggrecan degradation by aggrecanases

2002 ◽  
Vol 364 (1) ◽  
pp. 181-190 ◽  
Author(s):  
Heather STANTON ◽  
Linh UNG ◽  
Amanda J. FOSANG
Keyword(s):  
Matrix Metalloproteinase ◽  
Conditioned Medium ◽  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
Cartilage Explants ◽  
Dependent Manner ◽  
Collagen Binding ◽  
Matrix Metalloproteinase 13 ◽  
Cultured Chondrocytes ◽  
Stimulation Of

Fragments of fibronectin occur naturally in vivo and are increased in the synovial fluid of arthritis patients. We have studied the 45kDa fragment (Fn-f 45), representing the N-terminal collagen-binding domain of fibronectin, for its ability to modulate the expression of metalloproteinases by porcine articular chondrocytes in vitro. We report that stimulation of cultured chondrocytes, or cartilage explants, with Fn-f 45 increased the levels of matrix metalloproteinase-13 (MMP-13; collagenase-3) released into the conditioned medium in a dose-dependent manner. Increased levels of MMP-13 were due to stimulation of MMP-13 synthesis, rather than release of MMP-13 from accumulated matrix stores. Fn-f 45 also stimulated the synthesis of MMP-3 (stromelysin-1) from cultured chondrocytes and cartilage cultures. The Fn-f 45-induced increase in MMP-3 and MMP-13 synthesis occurred via an interleukin 1-independent mechanism, since the receptor antagonist of interleukin-1 was unable to block the increased synthesis. The gelatinases, MMP-2 and MMP-9, were not modulated by Fn-f 45 in these culture systems. Fn-f 45 also stimulated the release of aggrecan from cartilage explants into conditioned medium. Neoepitope antibodies specific for aggrecan fragments generated by MMPs or aggrecanases showed that the Fn-f 45-induced aggrecan loss was mediated by aggrecanases, and not by MMPs. Extracts of cultured cartilage contained elevated levels of the aggrecanase-derived ITEGE373-G1 domain, whereas levels of the matrix metalloproteinase-derived DIPEN341-G1 domain were unchanged. These studies show that Fn-f 45 can induce a catabolic phenotype in articular chondrocytes by up-regulating the expression of metalloproteinases specific for the degradation of collagen and aggrecan.

scholarly journals Effect of Moxibustion on the Serum Levels of MMP-1, MMP-3, and VEGF in Patients with Rheumatoid Arthritis

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Zeyun Yu ◽  
Yingni Wang ◽  
Yuan Li ◽  
Chenxi Liao ◽  
Jingyang Dai ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Treatment Group ◽  
Matrix Metalloproteinase ◽  
Clinical Symptoms ◽  
Interleukin 1 ◽  
Serum Levels ◽  
Control Group ◽  
Matrix Metalloproteinase 3 ◽  
Disease Markers ◽  
Matrix Metalloproteinase 1

Background. Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, which will eventually lead to joints deformity and functional damage. The aim of this research is to evaluate the effect of moxibustion on the serum indicators related to bone and cartilage metabolism, matrix metalloproteinase 1 (MMP-1), matrix metalloproteinase 3 (MMP-3), and vascular endothelial growth factor (VEGF) in patients with RA and to explore the mechanism of moxibustion in the treatment of RA. Methods. We recruited 70 RA patients who met the inclusion criteria, and they were randomly divided into two groups, a treatment group and a control group in equal ratio. The control group took methotrexate, folate, or leflunomide orally, while the treatment group received methotrexate, folate, or leflunomide orally and moxibustion at ST36 (Zusanli), BL23 (Shen shu), and Ashi points. We compared the clinical symptoms, RA serological disease markers and serum contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), MMP-1, MMP-3, and VEGF of RA patients before and after treatment. Results. (1) The clinical symptoms and RA serological disease markers of the two groups improved after treatment (P < 0.05), while the clinical symptoms of the treatment group were significantly improved in comparison with the control group (P < 0.05). (2) The levels of IL-1β, TNF-α, and VEGF decreased in both groups after treatment (P < 0.05), but the treatment group was significantly decreased compared with the control group (P < 0.05). (3) There were significant differences in MMP-1 and MMP-3 contents after treatment in the treatment group (P < 0.05, P < 0.05), while there were no significant differences in the control group (P > 0.05, P > 0.05). Above all, the contents of IL-1β, TNF-α, MMP-1, MMP-3, and VEGF in the treatment group decreased more significantly than those in the control group (P < 0.05). Conclusion. The improvement effect of moxibustion on the clinical symptoms of RA patients may be related to influence on the contents of IL-1β, TNF-α, MMP-1, MMP-3, and VEGF, and moxibustion may play a potential role in bone protection.

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