scholarly journals Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritis

2002 ◽  
Vol 46 (10) ◽  
pp. 2613-2624 ◽  
Author(s):  
Patrick Garnero ◽  
Xavier Ayral ◽  
Jean-Charles Rousseau ◽  
S. Christgau ◽  
Linda J. Sandell ◽  
...  
Keyword(s):  
Knee Osteoarthritis ◽  
Collagen Synthesis ◽  
Joint Damage ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Synthesis And Degradation

scholarly journals Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Heloisa Einloft Palma ◽  
Miguel Gallio ◽  
Gabriele Biavaschi da Silva ◽  
Camila Cantarelli ◽  
Kalyne Bertolin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Collagen Synthesis ◽  
Platelet Rich Plasma ◽  
Joint Damage ◽  
Type Ii Collagen ◽  
Control Group ◽  
Type Ii ◽  
Healthy Cartilage ◽  
Pro Inflammatory Cytokines

ABSTRACT: In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group when the expressions of MMP9, MMP13, IL-6 and ACAN genes were evaluated (P<0.05). With these results, we can suggest that the treatments were not deleterious to equine cultured chondrocyte, once they did not stimulate MMPs and IL-6 synthesis or caused changes in ACAN.

Correlation Between Muscle Strength and Functional Improvement After a Neuromuscular Electrical Strengthening Associated with Undenatured Type II Collagen in Knee Osteoarthritis

2021 ◽  
Vol 3 (5) ◽  
pp. 1122-1132
Author(s):  
Ana Paula Costa ◽  
Carlos Monteiro ◽  
Verine Cunha Teixeira ◽  
Bruno da Silva Schwarstzhoupt ◽  
Patrícia Mota Ferreira ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Knee Osteoarthritis ◽  
Type Ii Collagen ◽  
Functional Improvement ◽  
Type Ii ◽  
Undenatured Type Ii Collagen

scholarly journals A Novel High Sensitivity Type II Collagen Blood-Based Biomarker, PRO-C2, for Assessment of Cartilage Formation

2018 ◽  
Vol 19 (11) ◽  
pp. 3485 ◽  
Author(s):  
Yunyun Luo ◽  
Yi He ◽  
Ditte Reker ◽  
Natasja Gudmann ◽  
Kim Henriksen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Growth Factor ◽  
Knee Osteoarthritis ◽  
High Sensitivity ◽  
Type Ii Collagen ◽  
Cartilage Explants ◽  
Type Ii ◽  
Serum Samples ◽  
Human Cartilage ◽  
Cartilage Formation

N-terminal propeptide of type II collagen (PIINP) is a biomarker reflecting cartilage formation. PIINP exists in two main splice variants termed as type IIA and type IIB collagen NH2-propeptide (PIIANP, PIIBNP). PIIANP has been widely recognized as a cartilage formation biomarker. However, the utility of PIIBNP as a marker in preclinical and clinical settings has not been fully investigated yet. In this study, we aimed to characterize an antibody targeting human PIIBNP and to develop an immunoassay assessing type II collagen synthesis in human blood samples. A high sensitivity electrochemiluminescence immunoassay, hsPRO-C2, was developed using a well-characterized antibody against human PIIBNP. Human cartilage explants from replaced osteoarthritis knees were cultured for ten weeks in the presence of growth factors, insulin-like growth factor 1 (IGF-1) or recombinant human fibroblast growth factor 18 (rhFGF-18). The culture medium was changed every seven days, and levels of PIIBNP, PIIANP, and matrix metalloproteinase 9-mediated degradation of type II collagen (C2M) were analyzed herein. Serum samples from a cross-sectional knee osteoarthritis cohort, as well as pediatric and rheumatoid arthritis samples, were assayed for PIIBNP and PIIANP. Western blot showed that the antibody recognized PIIBNP either as a free fragment or attached to the main molecule. Immunohistochemistry demonstrated that PIIBNP was predominately located in the extracellular matrix of the superficial and deep zones and chondrocytes in both normal and osteoarthritic articular cartilage. In addition, the hsPRO-C2 immunoassay exhibits acceptable technical performances. In the human cartilage explants model, levels of PIIBNP, but not PIIANP and C2M, were increased (2 to 7-fold) time-dependently in response to IGF-1. Moreover, there was no significant correlation between PIIBNP and PIIANP levels when measured in knee osteoarthritis, rheumatoid arthritis, and pediatric serum samples. Serum PIIBNP was significantly higher in controls (KL0/1) compared to OA groups (KL2/3/4, p = 0.012). The hsPRO-C2 assay shows completely different biological and clinical patterns than PIIANP ELISA, suggesting that it may be a promising biomarker of cartilage formation.

A Comparison of 3 Bioinks for 3D Bioprinting of Articular Cartilage

2021 ◽  
Author(s):  
Christel Henrionnet ◽  
Océane Messaoudi ◽  
Léa Pourchet ◽  
Pierre Gillet ◽  
Damien Loeuille ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Type Ii Collagen ◽  
Type Ii ◽  
3D Bioprinting ◽  
Matrix Synthesis ◽  
Cartilage Engineering ◽  
Low Viscosity ◽  
Promising Tool ◽  
Marrow Mesenchymal Stem Cells ◽  
Hematoxylin Eosin

Background: 3D printing has become a promising tool for cartilage engineering, combining 3D deposition of cells seeded in supporting biomaterials. Objective: Our goal was to evaluate the chondrogenic properties of three different bioinks, seeded with human bone marrow mesenchymal stem cells (bMSCs). Methods: The three different tested bioinks are seeded with 2 × 106 cells/mL bMSCs. The bioink#1 is composed of gelatin, fibrinogen, and very low viscosity alginate. The bioink#2 has the same composition, excepted for the alginate that is a low viscosity one. The bioink#3 is manufactured by CELLINK®. The cartilaginous substitutes were cultivated for 28 days in the presence of ITS vs TGF-ß1. The extracellular matrix synthesis is evaluated at D28 by histology (Hematoxylin-Eosin-Saffron & Alcian Blue) and immunostaining (type II collagen). Results: The bioink#1 better promoted type II collagen synthesis, although the three bioink were equipotent in terms of proteoglycan content. Despite its universal characteristics, the bioink#3 failed to encourage the hyaline-like matrix synthesis. Conclusion: The bioink#1 containing gelatin, fibrinogen, and very low viscosity seems to be the fittest of the three bio-inks to obtain a cartilaginous substitute presenting a remarkable matrix synthesis. This study confirms the importance of the choice of bioink for cartilage engineering.

scholarly journals The relationship between urinary C-Telopeptide fragments of type II collagen, knee joint load, pain, and physical function in individuals with medial knee osteoarthritis

2020 ◽  
Author(s):  
Luiz Fernando Approbato Selistre ◽  
Glaucia Helena Gonçalves ◽  
Fernando Augusto Vasilceac ◽  
Paula Regina Mendes da Silva Serrão ◽  
Theresa Helissa Nakagawa ◽  
...  
Keyword(s):  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Physical Function ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Medial Knee ◽  
Medial Knee Osteoarthritis ◽  
Knee Joint Load ◽  
Joint Load ◽  
The Relationship
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