A protein from Daudi cell line conditioned medium induces ovarian dysgenesis inDrosophila melanogaster

1992 ◽  
Vol 21 (2) ◽  
pp. 77-89 ◽  
Author(s):  
Noëlle Richard-Mercier ◽  
Michèle Thomas-Orillard ◽  
Madeleine Morinière ◽  
Patrick Porcheron ◽  
Daniel Soyez ◽  
...  
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Daudi Cell ◽  
Ovarian Dysgenesis

A variant form of laminin is responsible for the neurite promoting activity in conditioned medium from a squamous carcinoma cell line

1991 ◽  
Vol 16 ◽  
pp. 82
Author(s):  
Hidehiko Yoshida ◽  
Shuji Hasegawa ◽  
Hiroshi Kuromi ◽  
Hideki Tanzawa ◽  
Kikuo Takahashi ◽  
...  
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cell Line ◽  
Variant Form ◽  
Squamous Carcinoma Cell

Infusion of human embryonic kidney cell line conditioned medium reverses kainic acid induced hippocampal damage in mice

Cytotherapy ◽  
2014 ◽  
Vol 16 (12) ◽  
pp. 1760-1770 ◽  
Author(s):  
Anandh Dhanushkodi ◽  
Chaitra Venugopal ◽  
Pradeep Kumar K. Bevinahal ◽  
Kiranmai S. Rai ◽  
Raju R. Trichur ◽  
...  
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Kainic Acid ◽  
Kidney Cell ◽  
Hippocampal Damage ◽  
Kidney Cell Line ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cell

scholarly journals The effect of strontium ranelate on titanium particle-induced periprosthetic osteolysis regulated by WNT/β-catenin signaling in vivo and in vitro

2021 ◽  
Vol 41 (1) ◽  
Author(s):  
Bolun Wang ◽  
Haohui Guo ◽  
Tianxiang Geng ◽  
Kening Sun ◽  
Liang Zhang ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Aseptic Loosening ◽  
Cell Line ◽  
Conditioned Medium ◽  
Strontium Ranelate ◽  
Cell Model ◽  
Periprosthetic Osteolysis ◽  
A Cell

Abstract Aseptic loosening following periprosthetic osteolysis is the primary complication that limits the lifetime of total joint arthroplasty (TJA). The wear particles trigger a chronic inflammation response in the periprosthetic tissue and turn over the bone balance to bone resorption. The present study aimed to investigate the possible effect and mechanism of strontium ranelate (SR), a clinically safe drug for osteoporosis, on particle-induced periprosthetic osteolysis. Thirty-six female C57BL/6j mice underwent tibial Ti-nail implantation to establish an animal model of aseptic loosening. After 12 weeks, micro-CT results showed that strontium ranelate could inhibit periprosthetic bone resorption. In vitro, Ti particles were used to stimulate RAW264.7 cell line to collect conditioned medium, and co-culture MC3T3-E1 cell line with conditioned medium to establish a cell model of aseptic loosening. The results of alkaline phosphatase (ALP) detection, immunofluorescence, and flow cytometry demonstrated that strontium ranelate could regulate the expression of OPG/RANKL, promote differentiation and mineralization, and inhibit apoptosis in osteoblasts. Moreover, we revealed that SR’s exerted its therapeutic effect by down-regulating sclerostin, thereby activating the Wnt/β-catenin signal pathway. Therefore, this research suggests that strontium ranelate could be a potential drug for the prevention and treatment of particle-induced aseptic loosening post-TJA.

scholarly journals Limiting-dilution analysis of the effects of colony-stimulating factors, phytohemagglutinin, and hydrocortisone on hematopoietic progenitor cell growth

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1611-1618
Author(s):  
Y Takaue ◽  
CL Reading ◽  
AJ Roome ◽  
KA Dicke ◽  
S Tindle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Cell Growth ◽  
Cell Line ◽  
Conditioned Medium ◽  
Hematopoietic Progenitor ◽  
Colony Stimulating Factors ◽  
Gm Csf ◽  
Percoll Gradients ◽  
Limiting Dilution

The effects of colony-stimulating factors (CSFs), phytohemagglutinin (PHA), and hydrocortisone on the growth of human bone marrow hematopoietic progenitor cells (granulocyte-macrophage; GM) were analyzed in a limiting-dilution assay (LDA). Both low-density bone marrow cells separated by discontinuous Percoll gradients and a T cell- depleted and progenitor-enriched cell fraction obtained by the combination of counterflow elutriation centrifugation and Percoll gradients were examined in LDA. GCT (monocytoid cell line-conditioned medium containing GM-CSF), human placenta-conditioned medium, bladder carcinoma cell line 5637-conditioned medium (containing GM- and G-CSF), and recombinant CSF (G-CSF) directly induced proliferation of progenitors with single-hit kinetics. In some instances, however, PHA- stimulated lymphocyte-conditioned medium (containing G- and GM-CSF) showed deviation from single-hit kinetics, which demonstrated the presence of factor(s) suppressive to progenitor growth. In a T cell- depleted, progenitor-enriched fraction, PHA alone was found to suppress progenitor growth at a level as low as 100 ng/mL. The addition of hydrocortisone (10(-6) mol/L) increased the progenitor frequency but suppressed progenitor growth at 10(-4) mol/L. LDA appears to be a valuable method for exploring mechanisms of factors regulating hematopoietic cell growth.

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