Partial characterization of digestive tract proteinases from western corn rootworm larvae,Diabrotica virgifera

1992 ◽  
Vol 19 (4) ◽  
pp. 285-298 ◽  
Author(s):  
Jeffrey W. Gillikin ◽  
Steven Bevilacqua ◽  
John S. Graham
Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 783
Author(s):  
Man P. Huynh ◽  
Adriano E. Pereira ◽  
Ryan W. Geisert ◽  
Michael G. Vella ◽  
Thomas A. Coudron ◽  
...  

The western corn rootworm (WCR), Diabrotica virgifera LeConte, is the most serious pest of maize in the United States. In pursuit of developing a diet free of antibiotics for WCR, we characterized effects of thermal exposure (50–141 °C) and length of exposure on quality of WCRMO-2 diet measured by life history parameters of larvae (weight, molting, and survival) reared on WCRMO-2 diet. Our results indicated that temperatures had non-linear effects on performance of WCRMO-2 diet, and no impacts were observed on the length of time exposure. The optimum temperature of diet processing was 60 °C for a duration less than 30 min. A significant decline in development was observed in larvae reared on WCRMO-2 diet pretreated above 75 °C. Exposing WCRMO-2 diet to high temperatures (110–141 °C) even if constrained for brief duration (0.9–2.3 s) caused 2-fold reduction in larval weight and significant delays in larval molting but no difference in survival for 10 days compared with the control diet prepared at 65 °C for 10 min. These findings provide insights into the effects of thermal exposure in insect diet processing.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xu Hu ◽  
Chad J. Boeckman ◽  
Bin Cong ◽  
Joe P. Steimel ◽  
Nina M. Richtman ◽  
...  

An amendment to this paper has been published and can be accessed via a link at the top of the paper.


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