PCR-generated conspecific sodium channel gene probe for the house fly

1991 ◽  
Vol 16 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Douglas C. Knipple ◽  
Lori L. Payne ◽  
David M. Soderlund
Keyword(s):  
Sodium Channel ◽  
House Fly ◽  
Gene Probe ◽  
Channel Gene ◽  
Sodium Channel Gene

Developmental regulation of alternative exon usage in the house fly Vssc1 sodium channel gene

2002 ◽  
Vol 4 (3) ◽  
pp. 125-133 ◽  
Author(s):  
Si Lee ◽  
Patricia Ingles ◽  
Douglas Knipple ◽  
David Soderlund
Keyword(s):  
Sodium Channel ◽  
Developmental Regulation ◽  
House Fly ◽  
Alternative Exon ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Exon Usage

scholarly journals Permethrin Resistance in Aedes aegypti Affects Aspects of Vectorial Capacity

Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 71
Author(s):  
Tse-Yu Chen ◽  
Chelsea T. Smartt ◽  
Dongyoung Shin
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Mosquito Control ◽  
Vector Competence ◽  
Vectorial Capacity ◽  
Parental Population ◽  
Control Programs ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Specific Pcr

Aedes aegypti, as one of the vectors transmitting several arboviruses, is the main target in mosquito control programs. Permethrin is used to control mosquitoes and Aedes aegypti get exposed due to its overuse and are now resistant. The increasing percentage of permethrin resistant Aedes aegypti has become an important issue around the world and the potential influence on vectorial capacity needs to be studied. Here we selected a permethrin resistant (p-s) Aedes aegypti population from a wild Florida population and confirmed the resistance ratio to its parental population. We used allele-specific PCR genotyping of the V1016I and F1534C sites in the sodium channel gene to map mutations responsible for the resistance. Two important factors, survival rate and vector competence, that impact vectorial capacity were checked. Results indicated the p-s population had 20 times more resistance to permethrin based on LD50 compared to the parental population. In the genotyping study, the p-s population had more homozygous mutations in both mutant sites of the sodium channel gene. The p-s adults survived longer and had a higher dissemination rate for dengue virus than the parental population. These results suggest that highly permethrin resistant Aedes aegypti populations might affect the vectorial capacity, moreover, resistance increased the survival time and vector competence, which should be of concern in areas where permethrin is applied.

scholarly journals Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India

2018 ◽  
Vol 12 (1) ◽  
pp. e0006192 ◽  
Author(s):  
Moytrey Chatterjee ◽  
Sudeep Ballav ◽  
Ardhendu K. Maji ◽  
Nandita Basu ◽  
Biplab Chandra Sarkar ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Aedes Albopictus ◽  
West Bengal ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene
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