Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line

2001 ◽  
Vol 46 (1-2) ◽  
pp. 36-47
Author(s):  
P.J. Hatt ◽  
C. Liebon ◽  
M. Morinière ◽  
H. Oberlander ◽  
P. Porcheron
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Lepidopteran Cell Line

Effects of growth factors EGF and TGF-β in the endometrial adenocarcinoma cell line HEC 1B

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S236 ◽  
Author(s):  
T. STROWITZKI ◽  
M. WIMBAUER ◽  
D. PFEIFFER ◽  
P. SCHEIDEL
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Endometrial Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell

scholarly journals The effects of various hormones and growth factors on the growth of human insulin-producing cell line in serum-free medium

1997 ◽  
Vol 29 (4) ◽  
pp. 209-216 ◽  
Author(s):  
Jae-Jeong Lee ◽  
Jai-Hyun Kwon ◽  
Yong Keun Park ◽  
Ohoak Kwon ◽  
Tai-Wook Yoon
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Human Insulin ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Stem cell factor influences the proliferation and erythroid differentiation of the MB-02 human erythroleukemia cell line by binding to a high-affinity c-kit receptor

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 436-444 ◽  
Author(s):  
VC Broudy ◽  
DA Morgan ◽  
N Lin ◽  
KM Zsebo ◽  
FW Jacobsen ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Stem Cell ◽  
Growth Factors ◽  
Cell Line ◽  
Stem Cell Factor ◽  
Erythroid Differentiation ◽  
Gm Csf ◽  
High Affinity ◽  
Kit Receptor ◽  
Erythroleukemia Cell

Abstract Stem cell factor (SCF) acts in synergy with other growth factors such as erythropoietin (Epo), granulocyte-macrophage colony-stimulating factor (GM-CSF), or interleukin-3 (IL-3), to stimulate the growth of primitive hematopoietic cells. Because of the prominent role of CSF in the maintenance of normal erythropoiesis in vivo, we examined the effects of SCF on the Epo-inducible human erythroleukemia cell line MB- 02, and characterized the c-kit receptor in these cells. MB-02 cells cultured in serum-containing media do not survive in the absence of exogenous growth factors, but the addition of SCF, Epo, or IL-3 as a single factor enhanced MB-02 survival. Furthermore, in the presence of Epo, SCF (5 to 25 ng/mL) enhanced MB-02 proliferation in a dose- dependent manner, and increased the relative and absolute number of benzidine-positive cells generated. SCF also enhanced cell proliferation in the presence of either IL-3 or low concentrations of GM-CSF. A neutralizing anti-c-kit receptor monoclonal antibody (SR-1) blocked binding of 125I-SCF to MB-02 cells by 98%, and the effect of SCF on MB-02 growth, c-kit receptor-binding parameters were quantitated by equilibrium-binding experiments with 125I-SCF. MB-02 cells display a single class of high-affinity (50 pmol/L) c-kit receptors, with approximately 8,000 receptors per cell. The molecular weight of the c- kit receptor was determined by affinity cross-linking 125I-SCF to MB-02 cells. 125I-SCF-c-kit receptor complexes of approximately 155,000 and approximately 310,000 daltons were found, likely representing the monomeric and dimeric forms of the c-kit receptor. The binding affinity and molecular weight of the c-kit receptor on MB-02 cells are similar to those of normal human marrow cells. These results suggest that SCF synergizes with Epo to influence not only the proliferation but the erythroid differentiation of MB-02 cells. Thus, the MB-02 cell line may be a useful model in which to investigate the molecular mechanisms of SCF action.

Glial cell line-derived neurotrophic factor promotes sleep in rats and rabbits

2001 ◽  
Vol 280 (4) ◽  
pp. R1001-R1006 ◽  
Author(s):  
Tetsuya Kushikata ◽  
Takeshi Kubota ◽  
Jidong Fang ◽  
James M. Krueger
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Line ◽  
Glial Cell ◽  
Eye Movement ◽  
Neurotrophic Factor ◽  
Brain Temperature ◽  
Interleukin 1 ◽  
High Dose ◽  
Sleep Regulation

Various growth factors (e.g., growth hormone-releasing hormone, acidic fibroblast growth factor, nerve growth factor, brain-derived neurotrophic factor, and interleukin-1) are implicated in sleep regulation. It is hypothesized that neuronal activity enhances the production of such growth factors, and they in turn form part of the sleep regulatory mechanism. Glial cell line-derived neurotrophic factor (GDNF) promotes development, differentiation, maintenance, and regeneration of neurons, and its production is induced by well-characterized sleep regulatory substances such as interleukin-1 and tumor necrosis factor. Therefore, we investigated whether GDNF would promote sleep. Twenty-six male Sprague-Dawley rats and 30 male New Zealand White rabbits were surgically implanted with electroencephalogram (EEG) and electromyogram (EMG; rats only) electrodes, a brain thermistor, and a lateral intracerebroventricular cannula. The animals were injected intracerebroventricularly with pyrogen-free saline and on a separate day with one of the following doses of GDNF: 5, 50, and 500 ng in rabbits and 50 and 500 ng in rats. The EEG, brain temperature, EMG (in rats), and motor activity (in rabbits) were recorded for 23 h after the intracerebroventricular injection. GDNF (500-ng dose) increased the time spent in nonrapid eye movement sleep in both rats and rabbits. Rapid eye movement sleep was not affected by the lower doses of GDNF but was inhibited in rabbits after the high dose. EEG slow-wave activity was not affected by GDNF. The current results provide further evidence that various growth factors are involved in sleep regulation.

scholarly journals Production of Multiple Growth Factors by a Newly Established Human Thyroid Carcinoma Cell Line

1992 ◽  
Vol 83 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Kaoru Tohyama ◽  
Yataro Yoshida ◽  
Kensaku Ohashi ◽  
Emiko Sano ◽  
Hisataka Kobayashi ◽  
...  
Keyword(s):  
Growth Factors ◽  
Thyroid Carcinoma ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Human Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Thyroid Carcinoma Cell

Effects of growth factors on a human glioma cell line during invasion into rat brain aggregates in culture

1992 ◽  
Vol 84 (2) ◽  
pp. 190-197 ◽  
Author(s):  
M. Lund-Johansen ◽  
K. Forsberg ◽  
R. Bjerkvig ◽  
O. D. Laerum
Keyword(s):  
Growth Factors ◽  
Rat Brain ◽  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Human Glioma ◽  
Human Glioma Cell Line ◽  
Human Glioma Cell
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