CLONING AND EXPRESSING TRYPSIN MODULATING OOSTATIC FACTOR INChlorella desiccataTO CONTROL MOSQUITO LARVAE

2015 ◽  
Vol 91 (1) ◽  
pp. 17-36 ◽  
Author(s):  
Dov Borovsky ◽  
Andeas Sterner ◽  
Charles A. Powell
Keyword(s):  
Mosquito Larvae ◽  
Trypsin Modulating Oostatic Factor

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

Distribution and Diversity of Mosquito Larvae from Kopargaon Teshil, Dist. Ahmednagar (M.S.) India

2017 ◽  
Vol 3 (5) ◽  
pp. 1305-1310
Author(s):  
Ramdas Gokul Pawar ◽  
Kisan Dnyandeo Thete ◽  
Laxmikant Vitthalrao Shinde
Keyword(s):  
Mosquito Larvae

scholarly journals Mosquito Vector Production across Socio-Economic Divides in Baton Rouge, Louisiana

2021 ◽  
Vol 18 (4) ◽  
pp. 1420
Author(s):  
Rebeca de Jesús Crespo ◽  
Madison Harrison ◽  
Rachel Rogers ◽  
Randy Vaeth
Keyword(s):  
Low Income ◽  
Baton Rouge ◽  
Habitat Preferences ◽  
Abundant Species ◽  
Mosquito Larvae ◽  
Mosquito Vector ◽  
Mosquito Vectors ◽  
Low Income Groups ◽  
Socio Economic Factors

We investigated the role of socio-economic factors in the proliferation of mosquito vectors in two adjacent but socio-economically contrasting neighborhoods in Baton Rouge, LA, USA. We surveyed mosquito larvae habitat, mosquito larvae, and adult mosquitoes during the summer of 2020. We also evaluated the number of requests for mosquito abatement services in the years preceding the study for each area. While we did not find differences in terms of the most abundant species, Culex quinquefasicatus (F1,30 = 0.329, p = 0.57), we did find a higher abundance of mosquito habitats, particularly discarded tires, as well as larvae (z = 13.83, p < 0.001) and adults (F1,30 = 4.207, p = 0.049) of the species Aedes albopictus in the low-income neighborhood. In contrast, mosquito abatement requests were significantly higher in the high socio-economic neighborhood (z = −8.561, p < 0.001). This study shows how factors such as adjudicated properties, discarded tires and pest abatement requests can influence the abundance of mosquito vectors, disproportionately affecting low-income groups. This study also highlights how Aedes spp. may be better indicators than Culex spp. of socio-economic differences between nearby neighborhoods, due to their short flight range and habitat preferences, and this should be considered in future studies attempting to detect such disparities in the future.

scholarly journals Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Clarence M. Mang’era ◽  
Fathiya M. Khamis ◽  
Erick O. Awuoche ◽  
Ahmed Hassanali ◽  
Fidelis Levi Odhiambo Ombura ◽  
...  
Keyword(s):  
Immune Response ◽  
Anopheles Gambiae ◽  
Growth And Development ◽  
Leaf Extract ◽  
Expression Profiles ◽  
Sensu Stricto ◽  
Mosquito Larvae ◽  
Insect Vector ◽  
Murraya Koenigii ◽  
Transcriptomic Response

Abstract Background Insect growth regulators (IGRs) can control insect vector populations by disrupting growth and development in juvenile stages of the vectors. We previously identified and described the curry tree (Murraya koenigii (L.) Spreng) phytochemical leaf extract composition (neplanocin A, 3-(1-naphthyl)-l-alanine, lumiflavine, terezine C, agelaspongin and murrayazolinol), which disrupted growth and development in Anopheles gambiae sensu stricto mosquito larvae by inducing morphogenetic abnormalities, reducing locomotion and delaying pupation in the mosquito. Here, we attempted to establish the transcriptional process in the larvae that underpins these phenotypes in the mosquito. Methods We first exposed third-fourth instar larvae of the mosquito to the leaf extract and consequently the inherent phytochemicals (and corresponding non-exposed controls) in two independent biological replicates. We collected the larvae for our experiments sampled 24 h before peak pupation, which was 7 and 18 days post-exposure for controls and exposed larvae, respectively. The differences in duration to peak pupation were due to extract-induced growth delay in the larvae. The two study groups (exposed vs control) were consequently not age-matched. We then sequentially (i) isolated RNA (whole larvae) from each replicate treatment, (ii) sequenced the RNA on Illumina HiSeq platform, (iii) performed differential bioinformatics analyses between libraries (exposed vs control) and (iv) independently validated the transcriptome expression profiles through RT-qPCR. Results Our analyses revealed significant induction of transcripts predominantly associated with hard cuticular proteins, juvenile hormone esterases, immunity and detoxification in the larvae samples exposed to the extract relative to the non-exposed control samples. Our analysis also revealed alteration of pathways functionally associated with putrescine metabolism and structural constituents of the cuticle in the extract-exposed larvae relative to the non-exposed control, putatively linked to the exoskeleton and immune response in the larvae. The extract-exposed larvae also appeared to have suppressed pathways functionally associated with molting, cell division and growth in the larvae. However, given the age mismatch between the extract-exposed and non-exposed larvae, we can attribute the modulation of innate immune, detoxification, cuticular and associated transcripts and pathways we observed to effects of age differences among the larvae samples (exposed vs control) and to exposures of the larvae to the extract. Conclusions The exposure treatment appears to disrupt cuticular development, immune response and oxidative stress pathways in Anopheles gambiae s.s larvae. These pathways can potentially be targeted in development of more efficacious curry tree phytochemical-based IGRs against An. gambiae s.s mosquito larvae.

Simple method to detect and to isolate entomopathogenic fungi (Hypocreales) from mosquito larvae

2021 ◽  
pp. 107581
Author(s):  
Juscelino Rodrigues ◽  
Caroline Bergamini ◽  
Cristian Montalva ◽  
Richard A. Humber ◽  
Christian Luz
Keyword(s):  
Entomopathogenic Fungi ◽  
Mosquito Larvae ◽  
Simple Method

scholarly journals Different Hatching Rates of Floodwater Mosquitoes Aedes sticticus, Aedes rossicus and Aedes cinereus from Different Flooded Environments

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 279
Author(s):  
Anders Lindström ◽  
Disa Eklöf ◽  
Tobias Lilja
Keyword(s):  
Tap Water ◽  
Soil Samples ◽  
Mosquito Larvae ◽  
Retaining Structures ◽  
Retaining Structure ◽  
Large Numbers ◽  
Random Samples ◽  
Nuisance Species ◽  
Flooding Events ◽  
Hatching Rates

In the lower Dalälven region, floodwater mosquitoes cause recurring problems. The main nuisance species is Aedes (Ochlerotatus) sticticus, but large numbers of Aedes (Aedes) rossicus and Aedes (Aedes) cinereus also hatch during flooding events. To increase understanding of which environments in the area give rise to mosquito nuisance, soil samples were taken from 20 locations from four environmental categories: grazed meadows, mowed meadows, unkept open grassland areas and forest areas. In each location 20 soil samples were taken, 10 from random locations and 10 from moisture retaining structures, such as tussocks, shrubs, piles of leaves, logs, and roots. The soil samples were soaked with tap water in the lab, and mosquito larvae were collected and allowed to develop to adult mosquitoes for species identification. Fewer larvae hatched from mowed areas and more larvae hatched from moisture retaining structure samples than random samples. The results showed that Aedes cinereus mostly hatch from grazed and unkept areas and hatched as much from random samples as from structures, whereas Aedes sticticus and Aedes rossicus hatched from open unkept and forest areas and hatch significantly more from structure samples. When the moisture retaining structures in open unkept areas where Aedes sticticus hatched were identified it was clear that they hatched predominantly from willow shrubs that offered shade. The results suggest that Ae. sticticus and Ae. cinereus favor different flooded environments for oviposition.

Detection of Mosquito Larvae Using Convolutional Neural Network

2021 ◽  
Author(s):  
Meer Shadman Saeed ◽  
Syeda Fahima Nazreen ◽  
Syed Shah Sufi Azmat Ullah ◽  
Zannatul Ferdaus Rinku ◽  
Md. Abdur Rahman
Keyword(s):  
Neural Network ◽  
Convolutional Neural Network ◽  
Mosquito Larvae
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