MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES IN THE COTTON MIRID BUG,Apolygus lucorum

2014 ◽  
Vol 86 (2) ◽  
pp. 107-121 ◽  
Author(s):  
Yongan Tan ◽  
Liubin Xiao ◽  
Yang Sun ◽  
Jing Zhao ◽  
Lixin Bai ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Apolygus Lucorum ◽  
Membrane Bound ◽  
Mirid Bug

scholarly journals Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)

2001 ◽  
Vol 205 (2) ◽  
pp. 203-207 ◽  
Author(s):  
R. Csáki ◽  
T. Hanczár ◽  
L. Bodrossy ◽  
J.C. Murrell ◽  
K.L. Kovács
Keyword(s):  
Molecular Characterization ◽  
Methylococcus Capsulatus ◽  
Structural Genes ◽  
Membrane Bound

MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES OF THE WHITEFLY,Bemisia tabaci

2014 ◽  
Vol 85 (4) ◽  
pp. 216-233 ◽  
Author(s):  
Jia Wang ◽  
Wen-Bo He ◽  
Yun-Lin Su ◽  
Xiao-Li Bing ◽  
Shu-Sheng Liu
Keyword(s):  
Molecular Characterization ◽  
Bemisia Tabaci ◽  
Membrane Bound

Solubilization and molecular characterization of membrane-bound antigens shared by thymocytes and brain

1976 ◽  
Vol 6 (5) ◽  
pp. 333-340 ◽  
Author(s):  
R. Arndt ◽  
Rosemarie Stark ◽  
P. Klein ◽  
Angelika Müller ◽  
H. G. Thiele
Keyword(s):  
Molecular Characterization ◽  
Membrane Bound

scholarly journals Molecular characterization and transcriptional regulation of two types of H+-pyrophosphatases in the scuticociliate parasite Philasterides dicentrarchi

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
I. Folgueira ◽  
J. Lamas ◽  
R. A. Sueiro ◽  
J. M. Leiro
Keyword(s):  
Molecular Characterization ◽  
Genetic Expression ◽  
Molecular Weights ◽  
Native Proteins ◽  
Ciliate Species ◽  
Membrane Bound ◽  
Transmembrane Regions ◽  
Antiparasitic Drugs ◽  
Membrane Bound Enzymes

AbstractProton-translocating inorganic pyrophosphatases (H+-PPases) are an ancient family of membrane bound enzymes that couple pyrophosphate (PPi) hydrolysis to H+ translocation across membranes. In this study, we conducted a molecular characterization of two isoenzymes (PdVP1 and PdVP2) located in respectively the alveolar sacs and in the membranes of the intracellular vacuoles of a scuticociliate parasite (Philasterides dicentrarchi) of farmed turbot. We analyzed the genetic expression of the isoenzymes after administration of antiparasitic drugs and after infection in the host. PdVP1 and PdVP2 are encoded by two genes of 2485 and 3069 bp, which respectively contain 3 and 11 exons and express proteins of 746 and 810 aa of molecular mass 78.9 and 87.6 kDa. Topological predictions from isoenzyme sequences indicate the formation of thirteen transmembrane regions (TMRs) for PdVP1 and seventeen TMRs for PdVP2. Protein structure modelling indicated that both isoenzymes are homodimeric, with three Mg2+ binding sites and an additional K+ binding site in PdVP2. The levels of identity and similarity between the isoenzyme sequences are respectively 33.5 and 51.2%. The molecular weights of the native proteins are 158 kDa (PdVP1) and 178 kDa (PdVP2). The isoenzyme sequences are derived from paralogous genes that form a monophyletic grouping with other ciliate species. Genetic expression of the isoenzymes is closely related to the acidification of alveolar sacs (PdVP1) and intracellular vacuoles (PdVP2): antiparasitic drugs inhibit transcription, while infection increases transcription of both isoenzymes. The study findings show that P. dicentrarchi possesses two isoenzymes with H+-PPase activity which are located in acidophilic cell compartment membranes and which are activated during infection in the host and are sensitive to antiparasitic drugs. The findings open the way to using molecular modelling to design drugs for the treatment of scuticociliatosis.

scholarly journals Genome-scale screening and molecular characterization of membrane-bound transcription factors in Arabidopsis and rice

Genomics ◽  
2010 ◽  
Vol 95 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Sang-Gyu Kim ◽  
Sangmin Lee ◽  
Pil Joon Seo ◽  
Soon-Kap Kim ◽  
Jeong-Kook Kim ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Membrane Bound ◽  
Genome Scale
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