Localization of Bmi1 in osteoblast‐lineage cells during endochondral ossification

2021 ◽  
Author(s):  
Hiroaki Takebe ◽  
Kazuharu Irie ◽  
Akihiro Hosoya
Keyword(s):  
Endochondral Ossification ◽  
Osteoblast Lineage

scholarly journals Gli1 Protein Participates in Hedgehog-mediated Specification of Osteoblast Lineage during Endochondral Ossification

2012 ◽  
Vol 287 (21) ◽  
pp. 17860-17869 ◽  
Author(s):  
Hironori Hojo ◽  
Shinsuke Ohba ◽  
Fumiko Yano ◽  
Taku Saito ◽  
Toshiyuki Ikeda ◽  
...  
Keyword(s):  
Endochondral Ossification ◽  
Osteoblast Lineage

scholarly journals Activation of β–catenin-LEF/TCF signal pathway in chondrocytes stimulates ectopic endochondral ossification

2003 ◽  
Vol 11 (1) ◽  
pp. 36-43 ◽  
Author(s):  
J. Kitagaki ◽  
M. Iwamoto ◽  
J.-G. Liu ◽  
Y. Tamamura ◽  
M. Pacifci ◽  
...  
Keyword(s):  
Endochondral Ossification ◽  
Signal Pathway

scholarly journals PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yu-Ting Yen ◽  
May Chien ◽  
Pei-Yi Wu ◽  
Shih-Chieh Hung
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Mesenchymal Stem Cells ◽  
Bone Density ◽  
Endochondral Ossification ◽  
Bone Growth ◽  
Leptin Receptor ◽  
Trabecular Bone Density ◽  
Mature Adipocyte ◽  
Ossification Centers

AbstractIt has not been well studied which cells and related mechanisms contribute to endochondral ossification. Here, we fate mapped the leptin receptor-expressing (LepR+) mesenchymal stem cells (MSCs) in different embryonic and adult extremities using Lepr-cre; tdTomato mice and investigated the underling mechanism using Lepr-cre; Ppp2r1afl/fl mice. Tomato+ cells appear in the primary and secondary ossification centers and express the hypertrophic markers. Ppp2r1a deletion in LepR+ MSCs reduces the expression of Runx2, Osterix, alkaline phosphatase, collagen X, and MMP13, but increases that of the mature adipocyte marker perilipin, thereby reducing trabecular bone density and enhancing fat content. Mechanistically, PP2A dephosphorylates Runx2 and BRD4, thereby playing a major role in positively and negatively regulating osteogenesis and adipogenesis, respectively. Our data identify LepR+ MSC as the cell origin of endochondral ossification during embryonic and postnatal bone growth and suggest that PP2A is a therapeutic target in the treatment of dysregulated bone formation.

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