Animal Model of Chronic Tympanic Membrane Perforation

2019 ◽  
Vol 303 (3) ◽  
pp. 619-625 ◽  
Author(s):  
Michael Langston ◽  
Ariel Grobman ◽  
Stefania Goncalves ◽  
Simon I. Angeli
Keyword(s):  
Animal Model ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

A Novel Animal Model for Chronic Tympanic Membrane Perforation

2013 ◽  
Vol 149 (2_suppl) ◽  
pp. P232-P232
Author(s):  
Enrique G. Archundia ◽  
Rafael A. Zárate García ◽  
Cynthia Vargas ◽  
Eduardo Sanchez
Keyword(s):  
Animal Model ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

An Animal Model of Chronic Tympanic Membrane Perforation

1992 ◽  
Vol 106 (1) ◽  
pp. 47-55 ◽  
Author(s):  
C. Philip Amoils ◽  
Robert K. Jackler ◽  
Henry Milczuk ◽  
Kevin E. Kelly ◽  
Keli Cao
Keyword(s):  
Animal Model ◽  
Tympanic Membrane ◽  
Tympanic Membrane Perforation ◽  
Human Beings ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation ◽  
Initial Procedure ◽  
Growth Promoting ◽  
Acute Perforation ◽  
The Ideal

Previous investigations into the healing and reconstruction of tympanic membrane (TM) perforations have involved animal models with acute TM perforations. A problem with the acute TM perforation model is that most acute TM perforations will heal spontaneously, both in animals and human beings. A second inadequacy of acute perforation models is that they are not analogous to the salient problem in human beings: Longstanding TM perforation. The ideal animal model must have a TM perforation that is permanent, well-eplthelialized, and free from infection. The perforation must also be subtotal to preserve a rim of membrane for experimental manipulations, in the chinchilla, we have identified a hardy animal with a short, wide ear canal and relatively large tympanic membranes. Thermal myringectomy, followed by medial infolding of TM microflaps, has resulted in permanent, subtotal chronic TM perforations in the chinchilla animal model. Of the 19 chinchillas (38 TMs) perforated, chronic subtotal perforations were created in 32 ears, 6 to 8 weeks after the initial procedure (84% success). Persistent infection or TM regeneration despite reperforation was recorded in 6 ears (16%) failure). This model is currently being used to assess various biomembrane scaffolds impregnated with growth-promoting substances in the regeneration of a physiologically sound TM, initially in our animal model and then in human beings. We envision the development of a biomembrane disc impregnated with biorecombinant growth factors that may provide a simple office technique for the repair of chronic, non-infected TM perforations.

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