scholarly journals Fetal Development of the Incisive Canal, Especially of the Delayed Closure Due to the Nasopalatine Duct: A Study Using Serial Sections of Human Fetuses

2017 ◽  
Vol 300 (6) ◽  
pp. 1093-1103 ◽  
Author(s):  
Ji Hyun Kim ◽  
Kyoko Oka ◽  
Zhe Wu Jin ◽  
Gen Murakami ◽  
José Francisco Rodríguez-Vázquez ◽  
...  
Keyword(s):  
Fetal Development ◽  
Human Fetuses ◽  
Serial Sections ◽  
Incisive Canal ◽  
Delayed Closure

Fetal Development of Human Oral Epithelial Pearls with Special Reference to Their Stage-Dependent Changes in Distribution

2017 ◽  
Vol 54 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Ji Hyun Kim ◽  
Zhe Wu Jin ◽  
Shunichi Shibata ◽  
Jae Do Yang ◽  
Gen Murakami ◽  
...  
Keyword(s):  
Special Reference ◽  
Late Stage ◽  
Fetal Development ◽  
Human Fetuses ◽  
Serial Sections ◽  
Dental Lamina ◽  
Lower Jaw ◽  
Age Dependent ◽  
Upper Jaw ◽  
Oral Epithelial

Objective To access detailed distribution and age-dependent changes of oral epithelial pearls. Design Investigation and analysis with human fetal serial sections. Setting Institute of Embryology. Methods This study examined serial frontal sections of the upper and lower jaws of 19 human fetuses at 12 to 18 weeks and of the lower jaws of four late-stage fetuses. Results The upper jaw contained more than 20 midline and more than 60 lateral pearls greater than 20 μm in diameter, whereas the lower jaw contained fewer than 30 pearls of the same size. Midline pearls in the upper jaw were often cylindrical or rugby-ball shaped, whereas all pearls in the lower jaw were small and spherical. Epithelial pearls in the upper jaw started developing along the upper midline until 12 weeks; lateral pearls and additional midline pearls (or strictly, paramedian pearls) developed until 15 weeks. In the lower jaw, however, pearl development started at 18 weeks and was almost always from the dental lamina. Some of the fetuses assessed had an open nasopalatine canal without a duct, but there was no fibrous connection between this canal and pearls. Similarly, the lip frenulum or incisive suture was not connected with these pearls. Conclusion The timing and sequence of development suggest that postfusion rupture of the palate by midline pearls was unlikely.

scholarly journals Development of synovial membrane in the temporomandibular joint of the human fetus

2015 ◽  
Vol 59 (4) ◽  
Author(s):  
L.O. Carvalho de Moraes ◽  
R.C. Tedesco ◽  
L.A. Arraez-Aybar ◽  
O. Klein ◽  
J.R. Mérida-Velasco ◽  
...  
Keyword(s):  
B Cells ◽  
Temporomandibular Joint ◽  
Synovial Membrane ◽  
Human Fetus ◽  
Human Fetuses ◽  
Serial Sections ◽  
Type B ◽  
Synovial Lining ◽  
Immunohistochemical Expression ◽  
Temporomandibular Joints

The development of the synovial membrane was analyzed in serial sections of 21 temporomandibular joints of human fetuses at 9 to 13 weeks of gestation. Sections of two fetuses at 12 weeks of development were used to perform immunohistochemical expression of the markers CD68 and Hsp27 on the synovial lining. Macrophage-like type A and fibroblast-like type B cells, which express CD68 and Hsp27, respectively, were observed at the twelfth week of development. Our results suggest that the development of the synovial membrane is related to the vascularization of the joint and the formation of the articular cavities.

scholarly journals Topographical variations of the incisive canal and nasopalatine duct in human fetuses

2019 ◽  
Vol 52 (4) ◽  
pp. 426
Author(s):  
Ji Hyun Kim ◽  
Shunichi Shibata ◽  
Hiroshi Abe ◽  
Gen Murakami ◽  
José Francisco Rodríguez-Vázquez
Keyword(s):  
Human Fetuses ◽  
Incisive Canal

scholarly journals Maturation of Cerebral Connections and Fetal Behavior

2008 ◽  
Vol 2 (3) ◽  
pp. 80-86 ◽  
Author(s):  
Milos Judas ◽  
Ivica Kostovic
Keyword(s):  
Fetal Development ◽  
Human Fetuses ◽  
Synaptic Organization ◽  
Present Evidence ◽  
Neuronal Circuitry ◽  
Cortical Connections ◽  
Behavioural Patterns ◽  
Cerebral Involvement ◽  
Successive Phase ◽  
Prematurely Born Infants

Abstract Modern imaging methods enabled systematic studies of fetal behaviour as well as a continuation of that behaviour in prematurely born infants (for a review, see 1-4). The following question represents a great challenge for human developmental neurobiologist: what is the neurobiological basis of various behavioural patterns observed in human fetuses and preterm infants?2 First of all, it is essential to determine whether there is an early spontaneous (nonsensory- driven) activity and to what extent the cerebrum and the cerebral cortex may be involved. In addition, it is necessary to describe for each successive phase, the developmental status of neuronal circuitry and synaptic organization. In this review, we present evidence on the development of cortical connections during different phases of fetal development and evaluate a possible functional significance of cerebral involvement.

scholarly journals A study on histology of fetal liver

2015 ◽  
Vol 04 (01) ◽  
pp. 026-029
Author(s):  
Jaiswal A. ◽  
Sinha DN ◽  
Singh AK
Keyword(s):  
Kupffer Cells ◽  
Fetal Development ◽  
Special Function ◽  
Fetal Liver ◽  
Fetal Life ◽  
Human Fetuses ◽  
Histological Findings ◽  
Adult Liver ◽  
Normal Human ◽  
Insight Into

Abstract Background and aims : Background and aims : The liver during fetogenesis does not follow classical lobular pattern. Normal histology of the fetal liver at various stages of development was studied to get insight into the morphology of fetal liver and special function it performs in fetal life. Method: Dissection of 29 normal human fetuses was done and histological findings of liver were noted with respect to the age of fetus. The histology of fetal liver was studied using H & E stain and important features of fetal liver were studied. Result: Fetal liver histology is different from adult liver. Unlike adult liver, fetal liver shows hepatocytes arranged in sheets predominantly with cordlike pattern at places. The sinusoids are dilated and filled with hemopoietic cells, which could be appreciated at different stages of fetal development. The Kupffer cells were also noticed in fetal liver. Conclusion: The present study will be helpful in understanding the normal histology of fetal liver and add to the existing knowledge regarding development of fetal liver.

Abnormal Vomer Development in Human Fetuses with Isolated Cleft Palate

2004 ◽  
Vol 41 (5) ◽  
pp. 470-473 ◽  
Author(s):  
Lene Hansen ◽  
Dorrit Nolting ◽  
Gitte Holm ◽  
Birgit Fischer Hansen ◽  
Inger Kjaer
Keyword(s):  
Cleft Palate ◽  
Human Development ◽  
Toluidine Blue ◽  
Nasal Septum ◽  
Human Fetuses ◽  
Serial Sections ◽  
Isolated Cleft Palate

Objective The aim of this study was to elucidate the prenatal human development of the vomer with emphasis on the vomeral footplate and to assess vomeral morphology in fetuses with isolated cleft palate. Material and Methods Nine human fetuses of which four were normal (menstrual age [MA] 13 to 21 weeks) and five with isolated cleft palate (14 to 19 weeks MA) were studied. Midaxial cranial tissue blocks from the fetuses were cut frontally in 4μm serial sections. Sections were stained with toluidine blue in 30% ethanol. Results From 16 weeks MA, the vomeral footplate of normal fetuses was formed from bilateral ossifications located below a U-shaped vomeral body. Later in development, an osseous connection was found between the footplate and the vomeral body. Neither bilateral areas of ossification below the vomer nor a vomeral footplate was observed in isolated cleft palate fetuses. Conclusions In normal fetuses, the base or footplate of the vomeral bone appears from 16 weeks MA in frontal sections. In fetuses with isolated cleft palates, with no connection between the nasal septum and the maxillary processes, this vomeral footplate does not develop in the period observed (14 to 19 weeks MA).

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

Ultrastructural localization of neuropeptides in the rat brain

1978 ◽  
Vol 36 (2) ◽  
pp. 612-613
Author(s):  
F.W. Van Leeuwen
Keyword(s):  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Serial Sections ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Enzyme Method ◽  
Perfusion Fixation ◽  
Electron Microscopical ◽  
Unlabeled Antibody ◽  
Peroxidase Conjugate

In order to obtain specific and optimal ultrastructural localization of vasopressin and oxytocin in the hypothalamo-neurohypophyseal system of the rat, 2 staining procedures and several tissue treatments were evaluated using neurohypophyseal tissue. It appeared from these studies that post-embedding staining with the unlabeled antibody enzyme method developed by Sternberger allows greater dilution of the first antibody (anti-vasopressin, 1:4800) than the indirect procedure (1:320) using a peroxidase conjugate as second antibody. Immersion fixation with 4% formalin during 24 hours gave better results (general ultrastructure, immunoreactivity) than those obtained by perfusion fixation with 2.5% glutaraldehyde-1% paraformaldehyde or freeze substitution.Since no reliable specificity tests were performed at the electron microscopical level, tests were developed for antibodies against both vasopressin and oxytocin. For anti-vasopressin plasma neural lobes of homozygous Brattleboro rats, that are lacking vasopressin by a genet- ical defect, were used. For antibodies against both hormones serial sections were used that were alternately incubated with the antibodies.

Probing the ultrastructure of the mitotic and meiotic spindle in eggs: An expeditious approach based on semi-thin (0.25 μm) serial sections

1983 ◽  
Vol 41 ◽  
pp. 552-555
Author(s):  
Conly L. Rieder ◽  
S. Bowser ◽  
R. Nowogrodzki ◽  
K. Ross ◽  
G. Sluder
Keyword(s):  
Small Sample Size ◽  
Small Sample ◽  
Meiotic Spindle ◽  
Serial Sections ◽  
Mitotic Apparatus ◽  
Thin Sections ◽  
Cell Cycles ◽  
Ultrastructural Studies

Eggs have long been a favorite material for studying the mechanism of karyokinesis in-vivo and in-vitro. They can be obtained in great numbers and, when fertilized, divide synchronously over many cell cycles. However, they are not considered to be a practical system for ultrastructural studies on the mitotic apparatus (MA) for several reasons, the most obvious of which is that sectioning them is a formidable task: over 1000 ultra-thin sections need to be cut from a single 80-100 μm diameter egg and of these sections only a small percentage will contain the area or structure of interest. Thus it is difficult and time consuming to obtain reliable ultrastructural data concerning the MA of eggs; and when it is obtained it is necessarily based on a small sample size.We have recently developed a procedure which will facilitate many studies concerned with the ultrastructure of the MA in eggs. It is based on the availability of biological HVEM's and on the observation that 0.25 μm thick serial sections can be screened at high resolution for content (after mounting on slot grids and staining with uranyl and lead) by phase contrast light microscopy (LM; Figs 1-2).

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