scholarly journals Characterization of Primary Cilia Distribution and Morphology During Lactation, Stasis, and Involution in the Bovine Mammary Gland

2013 ◽  
Vol 296 (12) ◽  
pp. 1943-1953 ◽  
Author(s):  
Melanie J. Millier ◽  
Kuljeet Singh ◽  
C. Anthony Poole
Keyword(s):  
Mammary Gland ◽  
Primary Cilia ◽  
Bovine Mammary Gland

scholarly journals Purification and characterization of glucosidase II involved in N-linked glycoprotein processing in bovine mammary gland

1987 ◽  
Vol 247 (3) ◽  
pp. 563-570 ◽  
Author(s):  
S Saxena ◽  
K Shailubhai ◽  
B Dong-Yu ◽  
I K Vijay
Keyword(s):  
Mammary Gland ◽  
Polyclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Mammary Gland ◽  
Ph Optimum ◽  
Glucosidase Ii ◽  
Reducing Conditions ◽  
Glycoprotein Processing

Glucosidase II is an endoplasmic-reticulum-localized enzyme that cleaves the two internally alpha-1,3-linked glucosyl residues of the oligosaccharide Glc alpha 1----2Glc alpha 1----3Glc alpha 1----3Man5-9GlcNAc2 during the biosynthesis of asparagine-linked glycoproteins. We have purified this enzyme to homogeneity from the lactating bovine mammary gland. The enzyme is a high-mannose-type asparagine-linked glycoprotein with a molecular mass of approx. 290 kDa. Upon SDS/polyacrylamide-gel electrophoresis under reducing conditions, the purified enzyme shows two subunits of 62 and 64 kDa, both of which are glycosylated. The pH optimum is between 6.6 and 7.0. Specific polyclonal antibodies raised against the bovine mammary enzyme also recognize a similar antigen in heart, liver and the mammary gland of bovine, guinea pig, rat and mouse. These antibodies were used to develop a sensitive enzyme-linked immunosorbent assay for glucosidase II.

scholarly journals Primary cilia distribution and orientation during involution of the bovine mammary gland

2016 ◽  
Vol 99 (5) ◽  
pp. 3966-3978 ◽  
Author(s):  
J. Biet ◽  
C.A. Poole ◽  
K. Stelwagen ◽  
J.K. Margerison ◽  
K. Singh
Keyword(s):  
Mammary Gland ◽  
Primary Cilia ◽  
Bovine Mammary Gland
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