scholarly journals Lymphatic/Blood Endothelial Cell Connections at the Capillary Level in Adult Rat Mesentery

2010 ◽  
Vol 293 (10) ◽  
pp. 1629-1638 ◽  
Author(s):  
Jennifer L. Robichaux ◽  
Eleanor Tanno ◽  
Jeff W. Rappleye ◽  
Mariana Ceballos ◽  
William B. Stallcup ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Adult Rat ◽  
Rat Mesentery

scholarly journals Lymphatic/Blood Endothelial Cell Connections at the Capillary Level in Adult Rat Mesentery

2010 ◽  
Vol 293 (10) ◽  
pp. spc1-spc1
Author(s):  
Jennifer L. Robichaux ◽  
Eleanor Tanno ◽  
Jeff W. Rappleye ◽  
Mariana Ceballos ◽  
William B. Stallcup ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Adult Rat ◽  
Rat Mesentery

Isolation and characterization of human and rat cardiac microvascular endothelial cells

1993 ◽  
Vol 264 (2) ◽  
pp. H639-H652 ◽  
Author(s):  
M. Nishida ◽  
W. W. Carley ◽  
M. E. Gerritsen ◽  
O. Ellingsen ◽  
R. A. Kelly ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelium ◽  
Adult Rat ◽  
Isolation And Characterization ◽  
Ventricular Tissue ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Although reciprocal intercellular signaling may occur between endocardial or microvascular endothelium and cardiac myocytes, suitable in vitro models have not been well characterized. In this report, we describe the isolation and primary culture of cardiac microvascular endothelial cells (CMEC) from both adult rat and human ventricular tissue. Differential uptake of fluorescently labeled acetylated low-density lipoprotein (Ac-LDL) indicated that primary isolates of rat CMEC were quite homogeneous, unlike primary isolates of human ventricular tissue, which required cell sorting based on Ac-LDL uptake to create endothelial cell-enriched primary cultures. The endothelial phenotype of both primary isolates and postsort subcultured CMEC and their microvascular origin were determined by characteristic histochemical staining for a number of endothelial cell-specific markers, by the absence of cells with fibroblast or pericyte-specific cell surface antigens, and by rapid tube formation on purified basement membrane preparations. Importantly, [3H]-thymidine uptake was increased 2.3-fold in subconfluent rat microvascular endothelial cells 3 days after coculture with adult rat ventricular myocytes because of release of an endothelial cell mitogen(s) into the extracellular matrix, resulting in a 68% increase in cell number compared with CMEC in monoculture. Thus biologically relevant cell-to-cell interactions can be modeled with this in vitro system.

Analysis of primary valve structure along initial lymphatic networks in adult rat mesentery

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Walter L Murfee ◽  
Jeff W Rappleye ◽  
Geert W Schmid‐Schönbein
Keyword(s):  
Adult Rat ◽  
Rat Mesentery ◽  
Valve Structure

Effects of cyclooxygenase-1/cyclooxygenase-2 inhibition on leukocyte/endothelial cell interactions in the rat mesentery

2002 ◽  
Vol 440 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Sara Calatayud ◽  
Jane A. Mitchell ◽  
Mauro Perretti ◽  
Francesco Giuliano ◽  
Timothy D. Warner
Keyword(s):  
Endothelial Cell ◽  
Cyclooxygenase 2 ◽  
Cell Interactions ◽  
Cyclooxygenase 1 ◽  
Rat Mesentery

Absence of OX-43 antigen expression in invasive capillary sprouts: identification of a capillary sprout-specific endothelial phenotype

2004 ◽  
Vol 286 (1) ◽  
pp. H346-H353 ◽  
Author(s):  
Christopher R. Anderson ◽  
Ana M. Ponce ◽  
Richard J. Price
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Antigen Expression ◽  
Cell Phenotype ◽  
Membrane Glycoprotein ◽  
Double Labeling ◽  
Rat Mesentery ◽  
Endothelial Phenotype ◽  
Tissue Space ◽  
Double Immunolabeling

Endothelial cells exhibit a number of unique phenotypes, some of which are angiogenesis dependent. To identify a capillary sprout-specific endothelial phenotype, we labeled angiogenic rat mesentery tissue using a microvessel and capillary sprout marker (laminin), selected endothelial cell markers (CD31, tie-2, and BS-I lectin), and the OX-43 monoclonal antibody, which recognizes a 90-kDa membrane glycoprotein of unknown function. In tissues that were stimulated through wound healing and compound 48/80 application, double-immunolabeling experiments with an anti-laminin antibody revealed that the OX-43 antigen was expressed strongly in all microvessels. However, the OX-43 antigen was completely absent from a large percentage (>85%) of the capillary sprouts that were invading the avascular tissue space. In contrast, sprouts that were introverting back into the previously vascularized tissue retained high levels of OX-43 antigen expression. Double-labeling experiments with endothelial markers indicated that the OX-43 antigen was expressed by microvessel endothelium but was absent from virtually all invasive capillary sprout endothelial cells. We conclude that the absence of OX-43 antigen expression marks a novel, capillary sprout-specific, endothelial cell phenotype. Endothelial cells of this phenotype are particularly abundant in capillary sprouts that invade avascular tissue during angiogenesis.

Role of mast cells in oxidized low-density lipoprotein-induced microvascular dysfunction

1996 ◽  
Vol 271 (5) ◽  
pp. H1795-H1800 ◽  
Author(s):  
L. Liao ◽  
D. N. Granger
Keyword(s):  
Mast Cell ◽  
Endothelial Cell ◽  
Oxidized Ldl ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Microvascular Dysfunction ◽  
Mast Cell Degranulation ◽  
Leukocyte Rolling ◽  
Rat Mesentery ◽  
Albumin Leakage

Previous studies demonstrated that human low-density lipoprotein (LDL) oxidized with Cu2+ promotes leukocyte-endothelial cell adhesion, mast cell degranulation, and albumin extravasation in rat mesentery. The objective of this study was to determine whether the mast cell degranulation elicited by oxidized LDL accounts for the accompanying microvascular responses. Leukocyte rolling, adherence, and emigration, fluorescein isothiocyanate-albumin leakage, and mast cell degranulation were monitored in rat mesentery before and during local intra-arterial infusion of either normal LDL (nLDL) or copper-oxidized LDL (CuLDL). Infusion of CuLDL, but not nLDL, elicited significant increases in leukocyte rolling, adherence and emigration, albumin leakage, and mast cell degranulation. Pretreatment with the mast cell-stabilizing agent ketotifen or superfusion of the mesenteric microcirculation with iodoxamide significantly reduced CuLDL-induced mast cell degranulation. The mast cell stabilization was accompanied by attenuated leukocyte-endothelial cell adhesion and albumin leakage responses to CuLDL. The results of this study indicate that 1) CuLDL-induced microvascular dysfunction (albumin leakage) involves the activation of mast cells, and 2) the protective action of mast cell stabilizers may be related to their ability to blunt CuLDL-induced leukocyte-endothelial cell interactions in postcapillary venules.

scholarly journals An angiogenesis model for investigating multicellular interactions across intact microvascular networks

2013 ◽  
Vol 304 (2) ◽  
pp. H235-H245 ◽  
Author(s):  
Peter C. Stapor ◽  
Mohammad S. Azimi ◽  
Tabassum Ahsan ◽  
Walter L. Murfee
Keyword(s):  
Endothelial Cell ◽  
Complex Dynamics ◽  
Wistar Rat ◽  
Class Iii ◽  
Rat Mesentery ◽  
Platelet Endothelial Cell Adhesion ◽  
Before And After ◽  
Functional Blocking ◽  
Multicellular Interactions ◽  
Angiogenesis Model

Developing therapies aimed at manipulating microvascular remodeling requires a better understanding of angiogenesis and how angiogenesis relates to other network remodeling processes, such as lymphangiogenesis and neurogenesis. The objective of this study was to develop an angiogenesis model that enables probing of multicellular and multisystem interactions at the molecular level across an intact adult microvascular network. Adult male Wistar rat mesenteric windows were aseptically harvested and cultured in serum-free minimum essential media. Viability/cytotoxicity analysis revealed that cells remain alive for at least 7 days. Immunohistochemical labeling at 3 days for platelet endothelial cell adhesion molecule (PECAM), neuron-glial antigen 2 (NG2), lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), and class III β-tubulin identified endothelial cells, pericytes, lymphatics, and nerves, respectively. Media supplemented with bFGF or VEGF induced an increase in endothelial cell sprouting off existing vessels. Endothelial cell sprouting in both growth factor groups was inhibited by targeting pericytes with NG2 functional blocking antibody. VEGF caused an increase in the number of lymphatic/blood endothelial cell connections compared with media alone or bFGF groups. Finally, the comparison of the same network before and after angiogenesis stimulated by the supplement of media with 20% serum identified the ability of disconnected endothelial segments to reconnect to nearby vessels. The results establish a novel in situ angiogenesis model for investigating the location of capillary sprouting within an intact network, the role of pericytes, lymphatic/blood endothelial cell interactions, and the fate of specific endothelial cell segments. The rat mesentery culture system offers a unique tool for understanding the complex dynamics associated with angiogenesis in an intact adult tissue.

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