scholarly journals A Preliminary Study of Solid Embryonic Cerebellar Graft Survival in Adult B6CBA Lurcher Mutant and Wild Type Mice

2009 ◽  
Vol 292 (12) ◽  
pp. 1986-1992 ◽  
Author(s):  
Jan Cendelín ◽  
Ivana Korelusová ◽  
FrantisÌŒek VozÌŒeh
Keyword(s):  
Graft Survival ◽  
Wild Type ◽  
Preliminary Study ◽  
Cerebellar Graft

Inhibition of toxicity and protofibril formation in the amyloid-β peptide β(25–35) using N-Methylated derivatives

2002 ◽  
Vol 30 (4) ◽  
pp. 537-542 ◽  
Author(s):  
A. J. Doig ◽  
E. Hughes ◽  
R. M. Burke ◽  
T. J. Su ◽  
R. K. Heenan ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Outer Edge ◽  
Amyloid Β Peptide ◽  
Wild Type ◽  
Diphenyltetrazolium Bromide ◽  
Amyloidogenic Peptides ◽  
Β Amyloid ◽  
Preliminary Study ◽  
Fibril Morphology ◽  
Derivatives Of

β(25–35) is a fragment of β-amyloid that retains its wild-type properties. N-methylated derivatives of β(25–35) can block hydrogen bonding on the outer edge of the assembling amyloid, so preventing the aggregation and inhibiting the toxicity of the wild-type peptide. The effects are assayed by Congo Red and thioflavin T binding, electron microscopy and an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] toxicity assay. N-methyl-Gly-25 has similar properties to the wild-type, while five other methylation sites have varying effects on prefolded fibrils and fibril assembly. In particular, N-methyl-Gly-33 is able to completely prevent fibril assembly and reduces the toxicity of prefolded amyloid. With N-methyl-Leu-34 the fibril morphology is altered and toxicity reduced. A preliminary study of β(25–35) structure in aqueous solution was made by small-angle neutron scattering (SANS). The protofibrillar aggregates are best described as a disc of radius 140 å and height 53 å (1 å = 0.1 nm), though the possibility of polydisperse aggregates cannot be ruled out. No aggregates form in the presence of N-methyl-Gly-33. We suggest that the use of N-methylated derivatives of amyloidogenic peptides and proteins could provide a general solution to the problem of amyloid deposition and toxicity and that SANS is an important technique for the direct observation of protofibril formation and destruction in solution.

scholarly journals Electrophysiological features of sleep in children with Kir4.1 channel mutations and Autism–Epilepsy phenotype: a preliminary study

SLEEP ◽  
2019 ◽  
Vol 43 (4) ◽  
Author(s):  
Federico Cucchiara ◽  
Paolo Frumento ◽  
Tommaso Banfi ◽  
Gianluca Sesso ◽  
Marco Di Galante ◽  
...  
Keyword(s):  
Case Control Study ◽  
Preliminary Finding ◽  
Autism Spectrum ◽  
Case Control ◽  
Clinical Applications ◽  
Slow Waves ◽  
Wild Type ◽  
Children With Asd ◽  
Preliminary Study ◽  
Control Study

Abstract Study Objectives Recently, a role for gain-of-function (GoF) mutations of the astrocytic potassium channel Kir4.1 (KCNJ10 gene) has been proposed in subjects with Autism–Epilepsy phenotype (AEP). Epilepsy and autism spectrum disorder (ASD) are common and complexly related to sleep disorders. We tested whether well characterized mutations in KCNJ10 could result in specific sleep electrophysiological features, paving the way to the discovery of a potentially relevant biomarker for Kir4.1-related disorders. Methods For this case–control study, we recruited seven children with ASD either comorbid or not with epilepsy and/or EEG paroxysmal abnormalities (AEP) carrying GoF mutations of KCNJ10 and seven children with similar phenotypes but wild-type for the same gene, comparing period-amplitude features of slow waves detected by fronto-central bipolar EEG derivations (F3-C3, F4-C4, and Fz-Cz) during daytime naps. Results Children with Kir4.1 mutations displayed longer slow waves periods than controls, in Fz-Cz (mean period = 112,617 ms ± SE = 0.465 in mutated versus mean period = 105,249 ms ± SE = 0.375 in controls, p < 0.001). An analog result was found in F3-C3 (mean period = 125,706 ms ± SE = 0.397 in mutated versus mean period = 120,872 ms ± SE = 0.472 in controls, p < 0.001) and F4-C4 (mean period = 127,914 ms ± SE = 0.557 in mutated versus mean period = 118,174 ms ± SE = 0.442 in controls, p < 0.001). Conclusion This preliminary finding suggests that period-amplitude slow wave features are modified in subjects carrying Kir4.1 GoF mutations. Potential clinical applications of this finding are discussed.

Fate of two types of cerebellar graft in wild type and cerebellar mutant mice

2014 ◽  
Vol 12 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Miroslava Čedíková ◽  
Zbyněk Houdek ◽  
Václav Babuška ◽  
Vlastimil Kulda ◽  
František Vožeh ◽  
...  
Keyword(s):  
Mutant Mice ◽  
Wild Type ◽  
Cerebellar Graft ◽  
Cerebellar Mutant

A PRELIMINARY STUDY OF THE CAROTENOIDS IN ACRASIS ROSEA

1966 ◽  
Vol 44 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Melvin S. Fuller ◽  
Rachel M. Rakatansky
Keyword(s):  
Rhodotorula Mucilaginosa ◽  
Wild Type ◽  
Preliminary Study ◽  
White Mutant

The amoebae of Acrasis rosea grown on a white mutant of Rhodotorula mucilaginosa contained three colored carotenoids. Two of these were xanthophylls, one being torulene with absorption maxima in hexane at 458, 484, and 518 mμ. The second xanthophyll, the most abundant pigment of the amoebae, was an unknown carotenoid with absorption maxima in hexane at 453, 477, and 508 mμ. A carotene with absorption maxima in hexane at 442, 468, and 500 mμ was also present in small amounts but not identified with any known carotene.It was demonstrated, contrary to a previous report, that the amoebae of A. rosea multiplied and became pigmented on carotenoid-lacking yeasts such as Saccharomyces cereviseae. On such colorless yeasts, the pigments were qualitatively the same as those isolated when A. rosea was grown on the colored wild-type and white mutant of R. mucilaginosa. The synthesis of torulene must then be accomplished, at least in its final stages, by the amoebae of A. rosea.

scholarly journals ABHD6 inhibition rescues a sex-dependent deficit in motor coordination in the HdhQ200/200 mouse model of Huntington’s disease

2021 ◽  
Author(s):  
Jessica K. Cao ◽  
Katie Viray ◽  
Myungsun Shin ◽  
Ku-Lung Hsu ◽  
Ken Mackie ◽  
...  
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Motor Coordination ◽  
Motor Behavior ◽  
Wild Type ◽  
Severe Phenotype ◽  
Behavioral Deficits ◽  
Immunohistochemistry Analysis ◽  
Pump Delivery ◽  
Preliminary Study

Abstract Huntington’s Disease is associated with motor behavior deficits that current therapeutics do not alleviate. This pilot study tested if pharmacological inhibition of a/b-hydrolase domain containing 6 (ABHD6), a multifunctional enzyme expressed in the striatum, rescues behavioral deficits in HdhQ200/200 mice. Previous work has shown that this model exhibits a reduction in spontaneous locomotion and motor coordination at 8 and 10 months of age, with a more severe phenotype in female mice. Semi-quantitative immunohistochemistry analysis indicated no change in striatal ABHD6 expression at 8 months of age, but a 40% reduction by 10 months in female HdhQ200/200 mice compared to female wild-type (WT) littermates. At 8 months of age, acute ABHD6 inhibition selectively rescued motor coordination deficits in female HdhQ200/200 mice without affecting WT performance. ABHD6 inhibition did not impact spontaneous locomotion, grip strength or overall weight in either group, showing that effects were specific to motor coordination. At 10 months of age, semi-chronic ABHD6 inhibition by osmotic pump delivery also rescued motor coordination deficits in female HdhQ200/200 mice without affecting female WT littermates. Our preliminary study suggests that ABHD6 inhibition selectively improves motor performance in female HdhQ200/200 mice.

scholarly journals A Preliminary Study on the Impact of UCK2 Knockdown in DLD-1 Colorectal Cells Treated with DHODH Inhibitor

2021 ◽  
Vol 50 (7) ◽  
pp. 1935-1946
Author(s):  
Mohamad Fairus Abdul Kadir ◽  
Puteri Shafinaz Abdul-Rahman ◽  
Kavitha Nellore ◽  
Shatrah Othman
Keyword(s):  
Dihydroorotate Dehydrogenase ◽  
Wild Type ◽  
Salvage Pathway ◽  
Reversal Effect ◽  
Proliferative Effect ◽  
Dna And Rna ◽  
Brequinar Sodium ◽  
Cell Proliferation Inhibition ◽  
Preliminary Study ◽  
The Impact

Brequinar sodium (BQR) is a well-studied inhibitor of the dihydroorotate dehydrogenase (DHODH) enzyme. Both the DHODH and uridine-cytidine kinase 2 (UCK2) enzymes have been reported to be over-expressed in cancer cells to maintain the cells high demand for DNA and RNA for their proliferation. In this study, we aim to further sensitize cells to the effects of BQR by knocking down the UCK2 activity. In DLD-1 UCK2 knockdown cells, no change in the sensitivity of cells to BQR was observed. Uridine is known to reverse the anti-proliferative effect of DHODH inhibitors via the salvage pathway. We observed abrogation of approximately 30% of the uridine reversal effect in UCK2 knockdown cells compared to the wild type cells. Our finding indicates that the loss of UCK2 activity in the salvage pathway did not enhance the BQR-mediated cell proliferation inhibition but it abrogates the uridine reversal in the cells.

Ultraviolet germicidal irradiation susceptibility of methicillin-resistant Staphylococcus aureus compared with methicillin-susceptible S. aureus

2015 ◽  
Vol 61 (11) ◽  
pp. 871-875 ◽  
Author(s):  
Christopher F. Green ◽  
Laura A. Elbe ◽  
Tyler D. Neal ◽  
John J. Lowe ◽  
Shawn G. Gibbs
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Human Pathogens ◽  
Wild Type ◽  
Methicillin Resistant ◽  
Ultraviolet Germicidal Irradiation ◽  
Significant Difference ◽  
The Mean ◽  
Preliminary Study ◽  
Type Strains

Antibiotic misuse and overuse in both the healthcare and agricultural fields have dramatically increased the prevalence of antibiotic resistance in human pathogens. Two strains of methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43330 and a wild-type) and 1 strain of methicillin-susceptible S. aureus (ATCC 25923) were challenged (9 runs in triplicate) in a preliminary study with ultraviolet germicidal irradiation (UVGI) doses ranging from 0.25 to 3.00 mJ/cm2. The mean percent kill was calculated for each strain when compared with the control plates (no exposure to UVGI). Then, each strain was challenged (22 runs in triplicate) with UVGI doses of 2.00, 2.50, and 3.00 mJ/cm2. The results suggest a difference between the doses required to disinfect surfaces with each strain. Assuming a standard error rate of α = 0.05, there was a significant difference in variance between the MRSA (ATCC 43330 and wild type) strains and the S. aureus (ATCC 25923) methicillin-susceptible strain.

scholarly journals Placenta-Specific Protein 1 Expression in Human Papillomavirus 16/18–Positive Cervical Cancers Is Associated With Tumor Histology

2017 ◽  
Vol 27 (4) ◽  
pp. 784-790 ◽  
Author(s):  
Eric J. Devor ◽  
Henry D. Reyes ◽  
Jesus Gonzalez-Bosquet ◽  
Akshaya Warrier ◽  
Susan A. Kenzie ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Specific Protein ◽  
Wild Type ◽  
Hpv 16 ◽  
Cervical Cancers ◽  
Preliminary Study ◽  
Histologic Types ◽  
Cervical Tumors ◽  
First Time

ObjectiveExpression of the trophoblast-specific gene placenta-specific protein 1 (PLAC1) has been detected in a wide variety of cancers. However, to date,PLAC1expression has not been shown in cervical cancer. We have carried out a preliminary study that shows for the first time thatPLAC1is expressed in cervical cancers.MethodsA total of 16 primary cervical tumors were obtained from patients shown to be human papillomavirus (HPV) 16/18 positive. Total cellular RNA, genomic DNA, and total protein were purified from each tumor. These materials were then used to determinePLAC1expression,TP53mutation status, andp53expression.ResultsThePLAC1expression was demonstrated in all 16 primary cervical tumors. The highest levels of expression were found in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Moreover, the proportion of totalPLAC1message coming from the P1 promoter, also termed the distal or cancer promoter, was significantly greater in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Finally, in spite of all 16 tumors being HPV-16/18 positive, 3 of 8 squamous cell cancers and 2 of 5 adenocarcinomas expressed wild-type p53 protein. Consistent with the recently shown suppression of the PLAC1P1 promoter by wild-type p53, these p53 positive tumors displayed among the lowest P1-specificPLAC1expression levels.ConclusionsThePLAC1expression has been demonstrated for the first time in cervical cancers. This preliminary study has further revealed a complex relationship betweenPLAC1expression, cervical cancer histologic type, p53, and HPV type that requires further investigation.

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