Relation between the trans-Golgi network and the Golgi stack on development of the Golgi apparatus of the ameloblast in developing rat molar tooth germs

1995 ◽  
Vol 241 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Saburou Matsuo ◽  
Kojiro Kurisu ◽  
Ken-Ichi Kiyomiya ◽  
Masaru Kurebe
Keyword(s):  
Golgi Apparatus ◽  
Molar Tooth ◽  
Golgi Stack ◽  
Trans Golgi Network ◽  
Tooth Germs ◽  
Golgi Network ◽  
Developing Rat ◽  
Rat Molar

Changes of lectin staining pattern of the Golgi stack during differentiation of the ameloblast in developing rat molar tooth germs

1993 ◽  
Vol 236 (2) ◽  
pp. 355-365 ◽  
Author(s):  
Saburou Matsuo ◽  
Hiroyuki Ichikawa ◽  
Kojiro Kurisu ◽  
Satoshi Wakisaka ◽  
Ken-Ichi Kiyomiya ◽  
...  
Keyword(s):  
Staining Pattern ◽  
Molar Tooth ◽  
Golgi Stack ◽  
Lectin Staining ◽  
Tooth Germs ◽  
Developing Rat ◽  
Rat Molar

Changes of cytochemical properties in the Golgi apparatus during in vivo differentation of the ameloblast in developing rat molar tooth germs

1992 ◽  
Vol 234 (4) ◽  
pp. 469-478 ◽  
Author(s):  
Saburou Matsuo ◽  
Hiroyuki Ichikawa ◽  
Satoshi Wakisaka ◽  
Michio Akai
Keyword(s):  
Golgi Apparatus ◽  
Molar Tooth ◽  
Tooth Germs ◽  
Developing Rat ◽  
Rat Molar

Cytochemical study of the Golgi apparatus and related organelles of the secretory ameloblasts of rat molar tooth germs cultured with and without colchicine

1988 ◽  
Vol 33 (6) ◽  
pp. 433-441 ◽  
Author(s):  
S. Matsuo ◽  
Y. Takano ◽  
S. Wakisaka ◽  
H. Ichikawa ◽  
S. Nishikawa ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Molar Tooth ◽  
Cytochemical Study ◽  
Tooth Germs ◽  
Rat Molar

Identification of a di-leucine motif within the C terminus domain of the Menkes disease protein that mediates endocytosis from the plasma membrane

1999 ◽  
Vol 112 (11) ◽  
pp. 1721-1732 ◽  
Author(s):  
M.J. Francis ◽  
E.E. Jones ◽  
E.R. Levy ◽  
R.L. Martin ◽  
S. Ponnambalam ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Golgi Apparatus ◽  
Transmembrane Domain ◽  
Menkes Disease ◽  
Cytoplasmic Domain ◽  
Endocytic Pathway ◽  
Site Directed Mutagenesis ◽  
Trans Golgi Network ◽  
C Terminus ◽  
Golgi Network

The protein encoded by the Menkes disease gene (MNK) is localised to the Golgi apparatus and cycles between the trans-Golgi network and the plasma membrane in cultured cells on addition and removal of copper to the growth medium. This suggests that MNK protein contains active signals that are involved in the retention of the protein to the trans-Golgi network and retrieval of the protein from the plasma membrane. Previous studies have identified a signal involved in Golgi retention within transmembrane domain 3 of MNK. To identify a motif sufficient for retrieval of MNK from the plasma membrane, we analysed the cytoplasmic domain, downstream of transmembrane domain 7 and 8. Chimeric constructs containing this cytoplasmic domain fused to the reporter molecule CD8 localised the retrieval signal(s) to 62 amino acids at the C terminus. Further studies were performed on putative internalisation motifs, using site-directed mutagenesis, protein expression, chemical treatment and immunofluorescence. We observed that a di-leucine motif (L1487L1488) was essential for rapid internalisation of chimeric CD8 proteins and the full-length Menkes cDNA from the plasma membrane. We suggest that this motif mediates the retrieval of MNK from the plasma membrane into the endocytic pathway, via the recycling endosomes, but is not sufficient on its own to return the protein to the Golgi apparatus. These studies provide a basis with which to identify other motifs important in the sorting and delivery of MNK from the plasma membrane to the Golgi apparatus.

Sign in / Sign up

Export Citation Format

Share Document