Scanning electron microscopic observations of human fetal kidney maturing in vivo and in serum-free organ culture

1993 ◽  
Vol 235 (3) ◽  
pp. 461-474 ◽  
Author(s):  
Normand Brièare ◽  
Pierre Magny
Keyword(s):  
Organ Culture ◽  
Fetal Kidney ◽  
Electron Microscopic ◽  
Serum Free ◽  
Scanning Electron Microscopic ◽  
Scanning Electron ◽  
Human Fetal Kidney

Cell interactions of Listeria monocytogenes L forms and peritoneal exudative cells in rats

1993 ◽  
Vol 39 (11) ◽  
pp. 1014-1021 ◽  
Author(s):  
L. Mihailova ◽  
N. Markova ◽  
T. Radoucheva ◽  
D. Veljanov ◽  
S. Radoevska
Keyword(s):  
Listeria Monocytogenes ◽  
Peritoneal Cavity ◽  
Electron Microscopic Examination ◽  
Lavage Fluid ◽  
Host Cells ◽  
Electron Microscopic ◽  
Cellular Defense ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Listeria monocytogenes 4b and its forms without cell walls (L forms of a protoplastic type) were used to study in vivo interactions with host cells. Samples of peritoneal lavage fluid were obtained from rats intraperitoneally inoculated at intervals between 1 and 15 days after challenge, for scanning electron microscopic, bacteriological, biochemical, and cytometrical investigations. Scanning electron microscopic examination revealed continuous adhesion of L forms on the macrophage surface up to 15 days after inoculation. The persistence of the L forms within the peritoneal cavity was also shown bacteriologically at all sample times, while the parental bacterial forms were isolated from the peritoneal cavity up to 7 days after challenge. The total count of peritoneal exudative cells determined by automated flow peroxidase cytometry peaked on the 15th day in animals infected with parental forms, while in animals infected with L forms the peak was lower and the macrophage population was predominant. The glycolytic and acid phosphatase activity of peritoneal exudative cells was two times higher in rats infected with L forms as compared with rats infected with the L. monocytogenes parental forms on the 3rd day after challenge. An understanding of the nature of the interactions between L forms of L. monocytogenes and peritoneal exudative cells found in vivo could be used to establish the influence of L forms on host cellular defense mechanisms.Key words: Listeria monocytogenes, L forms, peritoneal exudative cells, electron microscopy.

Microvascular Anastomosis: A Scanning Electron Microscopic Study

1980 ◽  
Vol 88 (3) ◽  
pp. 252-256 ◽  
Author(s):  
Douglas E. Mattox
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Microvascular Anastomosis ◽  
Electron Microscopic ◽  
Technical Errors ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

The single most important factor determining the patency of a microvascular anastomosis is the surgical precision with which it is performed. Inaccurately placed sutures, damage of the intima, exposed media and adventitia, and stenosis of the lumen at the site anastomosis all contribute to decreased patency rates. The first 50 consecutive microvascular anastomoses performed by a single microvascular surgeon were analyzed in vivo and with the scanning electron microscope. The frequency and significance of various technical errors are discussed. Scanning electron microscopy is recommended as a convenient and quick technique for assessing the evenness and accuracy of intimal apposition in microvascular anastomosis.

scholarly journals Adherence of enteroaggregative Escherichia coli to the ileal and colonic mucosa: an in vitro study utilizing the scanning electron microscopy

2011 ◽  
Vol 48 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Jacy Alves Braga de Andrade ◽  
Edna Freymüller ◽  
Ulysses Fagundes-Neto
Keyword(s):  
Escherichia Coli ◽  
Organ Culture ◽  
Colonic Mucosa ◽  
In Vitro Study ◽  
In Vitro Assays ◽  
Electron Microscopic ◽  
In Vitro Organ Culture ◽  
Scanning Electron

CONTEXT: Enteroaggregative Escherichia coli strains have been associated with persistent diarrhea in several developing countries. In vivo procedures with animal models, in vitro assays with cellular lines and in vitro organ culture with intestinal fragments have been utilized to study these bacteria and their pathogenicity. OBJECTIVE: The present experimental research assessed the pathogenic interactions of three enteroaggregative Escherichia coli strains, using the in vitro organ culture, in order to show the adherence to different regions of both, the ileal and the colonic mucosa and demonstrate possible mechanisms that could have the participation in the prolongation of diarrheiogenic process. METHODS: This study used intestinal fragments from terminal ileum and colon that were excised from pediatric patients undergoing intestinal surgeries and from adult patients that underwent to colonoscopic procedures. Each strain was tested with three intestinal fragments for each region. Tissue was fixed for scanning electron microscopic analysis. RESULTS: These bacteria colonized ileal and colonic mucosa in the typical stacked-brick configuration in the ileum and colon. In both regions, the strains were seen over a great amount of mucus and sometimes over the intact epithelium. In some regions, there is a probable evidence of effacement of the microvilli. It was possible to see adhered to the intestinal surface, bacteria fimbrial structures that could be responsible for the adherence process. CONCLUSION: In order to cause diarrhea, enteroaggregative Escherichia coli strains adhere to the intestinal mucosa, create a mucoid biofilm on the small bowel surface that could justify the digestive-absorptive abnormalities and consequently, prolonging the diarrhea.

Adult worm tegumental damage and egg-granulomas in praziquantel-resistant and -susceptible Schistosoma mansoni treated in vivo

2002 ◽  
Vol 76 (4) ◽  
pp. 327-333 ◽  
Author(s):  
Y.-S. Liang ◽  
G.C. Coles ◽  
J.-R. Dai ◽  
Y.-C. Zhu ◽  
M.J. Doenhoff
Keyword(s):  
Schistosoma Mansoni ◽  
Drug Resistant ◽  
Severe Damage ◽  
Drug Pressure ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Susceptible Control ◽  
Effect Of Treatment ◽  
Scanning Electron

AbstractThe effect of treatment with praziquantel (PZQ) on the tegument of adult Schistosoma mansoni worms and on liver egg-granulomas has been examined in mice infected with PZQ-resistant and -susceptible parasite isolates. Two PZQ-resistant S. mansoni isolates, one selected by passage in the laboratory under drug pressure and one from Senegal established from eggs excreted by an uncured patient, were compared with PZQ-susceptible control isolates. Scanning electron microscopic observations on the tegument of Schistosoma adult worms treated in vivo with PZQ showed that more severe damage was inflicted by PZQ on susceptible worms than on drug-resistant worms. Observations on the pathology of Schistosoma egg-granulomas in the liver of infected mice after treatment with PZQ indicated that eggs from susceptible control isolates were more sensitive to PZQ than those from drug-resistant isolates.

Sign in / Sign up

Export Citation Format

Share Document