A morphometric and computer-assisted three-dimensional reconstruction study of neural tube formation in chick embryos

1991 ◽  
Vol 231 (4) ◽  
pp. 425-436 ◽  
Author(s):  
Robert G. Nagele ◽  
Kevin T. Bush ◽  
Francis J. Lynch ◽  
Hsin-Yi Lee
2021 ◽  
pp. 002581722110183
Author(s):  
MA Kislov ◽  
M Chauhan ◽  
SN Zakharov ◽  
SV Leonov ◽  
YP Shakiryanova

Worldwide advances in computer techniques are not yet recognised in the practice of forensic medicine. A promising application is their use in making a three-dimensional reconstruction of the crime scene. This study analyses this technique in a homicide by firearm. Queries regarding the direction and number of shots, position of the victim inside the car when shot at and presence of the accused at the crime scene were answered by a scientific model. Similar reconstruction of the scene, nailing the accused in a heinous crime, has not previously been reported as a study or a case. The paper anticipates impetus to the growth of literature in criminology and forensic sciences. It will also expedite the delivery of justice based on scientific evidence in controversial causes of death.


1991 ◽  
Vol 37 (2) ◽  
pp. 151-160 ◽  
Author(s):  
A. Andreasen ◽  
A.M. Drewes ◽  
J.E. Assentoft ◽  
N.E. Larsen ◽  
H. Nielsen ◽  
...  

2003 ◽  
Vol 2 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Shawn R. Lockhart ◽  
Karla J. Daniels ◽  
Rui Zhao ◽  
Deborah Wessels ◽  
David R. Soll

ABSTRACT It was recently demonstrated that strains homozygous for either of the mating type-like loci MTL a and MTLα of Candida albicans undergo white-opaque switching and that expression of the opaque-phase phenotype greatly enhances mating between strains. Exploiting the latter property to obtain high-frequency mating, we have characterized the cell biology of the mating process of C. albicans. Employing continuous videomicroscopy, computer-assisted three-dimensional reconstruction of living cells, and fluorescence microscopy, we have monitored the mating-associated processes of conjugation, tube formation, fusion, budding, septum formation, and daughter cell development and the spatial and temporal dynamics of nuclear migration and division. From these observations, a model for the stages in C. albicans mating is formulated. The stages include shmooing, chemotropism of conjugation tubes, fusion of tubes and nuclear association, vacuole expansion and nuclear separation in the conjugation bridge, asynchronous nuclear division in the zygote, bud growth, nuclear migration into the daughter cell, septation, and daughter cell budding. Since there was no cytological indication of karyogamy, genetic experiments were performed to assess marker segregation. Recombination was not observed, suggesting that mating takes place in the absence of karyogamy between naturally occurring, homozygous a and α strains. This study provides the first description of the cell biology of the mating process of C. albicans.


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