Immunocytochemical localization of an oviductal zona pellucida glycoprotein in the oviductal epithelium of the golden hamster

1991 ◽  
Vol 229 (3) ◽  
pp. 305-314 ◽  
Author(s):  
Hiroyuki Abe ◽  
Taneaki Oikawa
Keyword(s):  
Zona Pellucida ◽  
Golden Hamster ◽  
Immunocytochemical Localization ◽  
Oviductal Epithelium

CatSper-null mutant spermatozoa are unable to ascend beyond the oviductal reservoir

2009 ◽  
Vol 21 (2) ◽  
pp. 345 ◽  
Author(s):  
Katharine Ho ◽  
Collin A. Wolff ◽  
Susan S. Suarez
Keyword(s):  
Zona Pellucida ◽  
High Amplitude ◽  
Null Mouse ◽  
Null Mutant ◽  
Wild Type ◽  
Timely Manner ◽  
Oviductal Epithelium ◽  
Sperm Reservoir

Sperm hyperactivation is characterised by high-amplitude, asymmetrical flagellar bending and is required to penetrate the oocyte zona pellucida. It was proposed that hyperactivation also enables spermatozoa to reach the oocyte by assisting escape from the oviductal sperm reservoir. To test this hypothesis, the behaviour of CatSper-null mouse spermatozoa in the oviduct was compared with that of spermatozoa from heterozygotes. CatSper–/– males are infertile because their spermatozoa fail to hyperactivate, whereas spermatozoa from CatSper+/– males have normal amounts of CatSper proteins and can hyperactivate. Males were mated with wild-type females on the morning of ovulation. Oviducts were obtained 1 or 4 h later, and behaviour of spermatozoa was examined using transillumination. At 1 h, null mutant spermatozoa remained attached by their heads to oviductal epithelium in the reservoir, whereas spermatozoa from heterozygotes detached from the oviductal epithelium after performing deep asymmetrical flagellar bends. At 4 h, 50 to 200 CatSper+/– spermatozoa were still seen in the oviducts; in contrast, only one CatSper–/– spermatozoon was found. CatSper–/– spermatozoa were lost from the oviducts after failing to detach from the epithelium in a timely manner, thus demonstrating that hyperactivation is required by spermatozoa to ascend beyond the oviductal reservoir.

The ultrastructure of implantation in the golden hamster (Cricetus auratus)

Development ◽  
1968 ◽  
Vol 19 (3) ◽  
pp. 341-345
Author(s):  
M. P. Young ◽  
J. T. Whicher ◽  
D. M. Potts
Keyword(s):  
Early Development ◽  
Zona Pellucida ◽  
Golden Hamster ◽  
Light Microscope ◽  
Uterine Epithelium

Previous work on the early development of the golden hamster includes the investigation of Ochs (1908), Graves (1945) and Ward (1948), all at the level of the light microscope. Austin (1963) has examined the ultrastructure of the oocytes of the golden hamster while Enders & Schlafke (1965) have observed the pre-implantation stages of pregnancy. The ultrastructure of implantation has been studied in two other species of myomorph rodents: the mouse (Potts, 1966a; Reinius, 1967) and the rat (Enders & Schlafke, 1967). Implantation is taken as beginning when the zona pellucida is lost and the trophoblast is in contact with the uterine epithelium throughout its circumference. This takes place at between 80–100 h post coitum. Previous studies have been made on specimens embedded in paraffin, and the shrinkage which occurs with this method of preservation has caused implantation to appear to begin considerably later than this:Graves (1945) gives it as beginning at 5 days, Ward(1948) as 4 days 8 h.

A monoclonal antibody reacting with the zona pellucida of the oviductal egg but not with that of the ovarian egg of the golden hamster

1987 ◽  
Vol 11 (3) ◽  
pp. 193-208 ◽  
Author(s):  
Yoshihiko Araki ◽  
Shoichiro Kurata ◽  
Taneaki Oikawa ◽  
Takao Yamashita ◽  
Masahiko Hiroi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Zona Pellucida ◽  
Golden Hamster
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