Three-dimensional computer-aided analysis of the intraganglionic topography of primary muscle afferent neurons in the rat

1990 ◽  
Vol 227 (4) ◽  
pp. 405-417 ◽  
Author(s):  
Jean-Marie Peyronnard ◽  
Jean-Pierre Messier ◽  
Martin Dubreuil ◽  
Louise Charron ◽  
France Lebel
2010 ◽  
Vol 29 (5) ◽  
pp. 751-757 ◽  
Author(s):  
Leeber Cohen ◽  
Kristie Mangers ◽  
William A. Grobman ◽  
Nina Gotteiner ◽  
Svena Julien ◽  
...  

2007 ◽  
Vol 98 (1) ◽  
pp. 214-223 ◽  
Author(s):  
Dean Dessem ◽  
Masayuki Moritani ◽  
Ranjinidevi Ambalavanar

Limited information is available on muscle afferent neurons with fine fibers despite their presumed participation in musculoskeletal disorders, including temporomandibular disorders. To study these neurons, intracellular recordings were made from the central axons of slowly conducting muscle afferent neurons in anesthetized rats. After intraaxonal impalement, axons were characterized by masseter nerve stimulation, receptive field testing, muscle stretching and intramuscular injection of hypertonic saline. Intracellular recordings were made from 310 axons (conduction velocity: 6.5–60M/s, mean = 27.3M/s; following frequency: 27–250 Hz, mean = 110Hz). No neurons responded to cutaneous palpation or muscle stretching. Some axons ( n = 34) were intracellularly stained with biotinamide. These neurons were classified as group II/III noxious mechanoreceptors because their mechanical threshold exceeded 15 mN, and conduction velocities ranged from 12 to 40.2M/s (mean = 25.3M/s). Two morphological types were recognized by using an object-based, three-dimensional colocalization methodology to locate synapses. One type (IIIHTMVp-Vc) possessed axon collaterals that emerged along the entire main axon and synapsed in the trigeminal principal sensory nucleus and spinal trigeminal subnuclei oralis (Vo), interpolaris (Vi), and caudalis (Vc). A second type (IIIHTMVo-Vc) possessed axon collaterals that synapsed only in caudal Vo, Vi, and Vc. Our previous studies show that muscle spindle afferent neurons are activated by innocuous stimuli and synapse in the rostral and caudal brain stem; here we demonstrate that nociceptive muscle mechanoreceptor afferent axons also synapse in rostral and caudal brain stem regions. Traditional dogma asserts that the most rostral trigeminal sensory complex exclusively processes innocuous somatosensory information, whereas caudal portions receive nociceptive sensory input; the data reported here do not support this paradigm.


Author(s):  
José Francisco Melo Júnior ◽  
Nathalie Jeanne Bravo-valenzuela ◽  
Luciano Marcondes Machado Nardozza ◽  
Alberto Borges Peixoto ◽  
Rosiane Mattar ◽  
...  

Abstract Objective To determine reference values for myocardial volume of the fetal heart using three-dimensional ultrasound with spatiotemporal image correlation (STIC) and virtual organ computer-aided analysis (VOCAL) and to assess their applicability in women with pregestational diabetes. Study Design This retrospective cross-sectional study included 177 normal pregnant women with fetuses between 200/7 and 336/7 weeks of age. Fetal cardiac volumes were collected using the STIC method, and myocardial volume was obtained by subtraction of the intracavitary volumes using the VOCAL 30-degree method. Intra- and interobserver reproducibility values were determined using the concordance correlation coefficient (CCC). Sixteen women with pregestational diabetes mellitus were evaluated for validation. Results There was a strong correlation between fetal myocardial volume and gestational age (R 2 = 0.82). Intra- and interobserver reproducibility values were excellent and moderate, respectively, with CCCs of 0.99 and 0.83, respectively. There was no significant difference in mean fetal myocardial volume between normal pregnant women and those with pregestational diabetes (p = 0.64). Conclusion Reference values for myocardial volume of the fetal heart were determined in normal pregnant women and were not statistically different from those in women with pregestational diabetes mellitus.


2011 ◽  
Vol 39 (6) ◽  
Author(s):  
Rafael Oliveira Cavalcante ◽  
Edward Araujo Júnior ◽  
Luciano Marcondes Machado Nardozza ◽  
Liliam Cristine Rolo ◽  
Antonio Fernandes Moron

Sign in / Sign up

Export Citation Format

Share Document