Changes in the pattern of intercellular transfer of Lucifer yellow between villus epithelial cells during postnatal development

1988 ◽  
Vol 222 (3) ◽  
pp. 282-288
Author(s):  
Hazel Cheng ◽  
Matthew Bjerknes
Keyword(s):  
Epithelial Cells ◽  
Postnatal Development ◽  
Lucifer Yellow ◽  
Intercellular Transfer

The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture

1990 ◽  
Vol 6 (1) ◽  
pp. 47-61 ◽  
Author(s):  
Irene V. Budunova ◽  
Leonid A. Mittelman ◽  
Gennady A. Belitsky
Keyword(s):  
Lucifer Yellow ◽  
Fibroblast Culture ◽  
Intercellular Transfer ◽  
Complete Carcinogens

scholarly journals Neuropeptides Exert Direct Effects on Rat Thymic Epithelial Cells in Culture

1998 ◽  
Vol 6 (1-2) ◽  
pp. 95-104 ◽  
Author(s):  
Gail M. Head ◽  
R. Mentlein ◽  
Birte Von Patay ◽  
J. E.G. Downing ◽  
Marion D. Kendall
Keyword(s):  
Epithelial Cells ◽  
Lucifer Yellow ◽  
Tritiated Thymidine ◽  
Single Cells ◽  
Thymic Epithelial Cells ◽  
Dye Coupling ◽  
Direct Effects ◽  
Adrenergic Agonist ◽  
Thymic Epithelium ◽  
Functional Aspects

To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue (which does) into single cells, some neuropeptides decrease dye coupling: 0.1 mM GABA (P< 0.0001), 100 nM NPY (P< 0.0001), 100 nM VIP (P< 0.001), 100 nM CGRP (P< 0.001), 100 nM SP (P< 0.01), and 0.1 mM histamine (P< 0.01), whereas 0.1 mM 5-HT, mM acetylcholine, and 1μM isoproterenol (β-adrenergic agonist) had no effect. Proliferation (incorporation of tritiated thymidine) was increased by CGRP (P= 0.004) and histamine (P< 0.02), but decreased by isoproterenol (P= 0.002), 5-HT (P= 0.003), and acetylcholine (P< 0.05). The percentage of multinucleate cells was decreased after isoproterenol (2.5%), and increased after 5-HT (21.3%), GABA (15%), and histamine (15.1%). Compared to controls, thymulin in the supernatant was decreased after challenge with acetylcholine (52%), isoproterenol (71%), 5-HT (73%), and histamine (84%). This study demonstrates direct effects of neuropeptides and neurotransmitters on functional aspects of cultured thymic epithelial cells.

Apical Na+/H+ exchange near the base of mouse colonic crypts

2002 ◽  
Vol 283 (1) ◽  
pp. C358-C372 ◽  
Author(s):  
Jingsong Chu ◽  
Shaoyou Chu ◽  
Marshall H. Montrose
Keyword(s):  
Epithelial Cells ◽  
Lucifer Yellow ◽  
Extracellular Fluid ◽  
Colonic Crypt ◽  
Fluid Flux ◽  
Functional Attributes ◽  
Colonic Crypts ◽  
Vectorial Transport ◽  
Crypt Base ◽  
Exchange Activity

Colonic crypts can absorb fluid, but the identity of the absorptive transporters remains speculative. Near the crypt base, the epithelial cells responsible for vectorial transport are relatively undifferentiated and often presumed to mediate only Cl−secretion. We have applied confocal microscopy in combination with an extracellular fluid marker [Lucifer yellow (LY)] or a pH-sensitive dye (2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein) to study mouse colonic crypt epithelial cells directly adjacent to the crypt base within an intact mucosal sheet. Measurements of intracellular pH report activation of colonocyte Na+/H+ exchange in response to luminal or serosal Na+. Studies with LY demonstrate the presence of a paracellular fluid flux, but luminal Na+ does not activate Na+/H+ exchange in the nonepithelial cells of the lamina propria, and studies with LY suggest that the fluid bathing colonocyte basolateral membranes is rapidly refreshed by serosal perfusates. The apical Na+/H+ exchange in crypt colonocytes is inhibited equivalently by luminal 20 μM ethylisopropylamiloride and 20 μM HOE-694 but is not inhibited by luminal 20 μM S-1611. Immunostaining reveals the presence of epitopes from NHE1 and NHE2, but not NHE3, in epithelial cells near the base of colonic crypts. Comparison of apical Na+/H+exchange activity in the presence of Cl− with that in the absence of Cl− (substitution by gluconate or nitrate) revealed no evidence of the Cl−-dependent Na+/H+ exchange that had been previously reported as the sole apical Na+/H+ exchange activity in the colonic crypt. Results suggest the presence of an apical Na+/H+ exchanger near the base of crypts with functional attributes similar to those of the cloned NHE2 isoform.

scholarly journals Plasticity of basal cells during postnatal development in the rat epididymis

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 455-469 ◽  
Author(s):  
Winnie W C Shum ◽  
Eric Hill ◽  
Dennis Brown ◽  
Sylvie Breton
Keyword(s):  
Epithelial Cells ◽  
Postnatal Development ◽  
Tight Junctions ◽  
Vas Deferens ◽  
Structural Plasticity ◽  
Basal Cells ◽  
Cytokeratin 5 ◽  
Rat Epididymis ◽  
Immunofluorescence Labeling

Our previous study has shown that basal cells sense luminal factors by forming a narrow body projection that can cross epithelial tight junctions. As a first step toward characterizing the structural plasticity of basal cells, in this study, we followed their appearance and morphology in the rat epididymis and vas deferens (VD) during postnatal development and examined their modulation by androgens in adulthood. Immunofluorescence labeling for cytokeratin 5 showed that basal cells are absent at birth. They progressively appear in a retrograde manner from the VD and cauda epididymis to the initial segments during the postnatal weeks PNW1–3. At the onset of differentiation, basal cells are in contact with the lumen and their nucleus is located at the same level as that of adjacent epithelial cells. Basal cells then position their nucleus to the base of the epithelium, and while some are still in contact with the lumen, others have a ‘dome-shaped’ appearance. At PNW5–6, basal cells form a loose network at the base of the epithelium, and luminal-reaching basal cells are rarely detected. The arrival of spermatozoa during PNW7–8 did not trigger the development of projections in basal cells. However, cells with a narrow luminal-reaching projection began to reappear between PNW8 and PNW12 in the corpus and the cauda. Treatment with flutamide from PNW10 to PNW12 significantly reduced the number of luminal-reaching basal cell projections. In summary, basal cells exhibit significant structural plasticity during differentiation. Fewer apical-reaching projections were detected after flutamide treatment in adulthood, indicating the role of androgens in the luminal-sensing function of basal cells.

Postnatal Development and Regulation of β-Hexosaminidase in Epithelial Cells of the Rat Epididymis

2004 ◽  
Vol 25 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Louis Hermo ◽  
Huzaifa I. Adamali ◽  
Jacquetta M. Trasler
Keyword(s):  
Epithelial Cells ◽  
Postnatal Development ◽  
Rat Epididymis

scholarly journals Identification and characteristics of postnatal development-related long non-coding RNAs under microbiota dependent condition

2020 ◽  
Author(s):  
Miaomiao Zheng ◽  
Binbin Zhang ◽  
Yidan Zhang ◽  
Tingting Sun ◽  
Baozhong Hu
Keyword(s):  
Epithelial Cells ◽  
Gut Microbiota ◽  
Postnatal Development ◽  
Intestinal Epithelial Cells ◽  
Development Stage ◽  
Intestinal Epithelial ◽  
Early Postnatal Development ◽  
Development Stages ◽  
Large Numbers ◽  
Non Coding Rnas

Abstract BackgroundThe interplay of long-non coding RNAs (lncRNAs) and the intestinal microbiota may serve as an essential role in intestinal development and homeostasis. Microbiota could regulate a large numbers of lncRNAs expression in intestinal epithelial cells. However, the associations between lncRNAs and microbiota during early postnatal development stages are still need to understand. MethodsIn present study, the microbial effects on lncRNA of intestinal epithelial cells (IECs) during postnatal development stage were investigated. ResultsWe identified gut microbiota-specific lncRNAs in diverse postnatal development stages including week 1, week 4 and week 12/16 of mice. A large proportion of gut microbiota-specific lncRNAs only were differential expressed in a single postnatal development stage. Up- and down-regulated gut microbiota-specific lncRNAs both showed consistent expression pattern. We also constructed gut microbiota-specific lncRNAs and coding genes interacted co-expressed networks. Functional analysis indicated that gut microbiota-specific lncRNAs were associated with ABC transporters. ConclusionsIn summary, the present study characterizes the landscape of lncRNAs associated with gut microbiota in different postnatal development stages. It provide assistance for exploring the relationships among lncRNAs, gut microbiota and postnatal development stages.

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