Tracheobronchial epithelium of the sheep: IV. Lectin histochemical characterization of secretory epithelial cells

1988 ◽  
Vol 222 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Andrew T. Mariassy ◽  
Charles G. Plopper ◽  
Judith A. St. George ◽  
Dennis W. Wilson
Keyword(s):  
Epithelial Cells ◽  
Tracheobronchial Epithelium ◽  
Histochemical Characterization

Tracheobronchial epithelium of the sheep: III. Carbohydrate histochemical and cytochemical characterization of secretory epithelial cells

1988 ◽  
Vol 221 (1) ◽  
pp. 540-549 ◽  
Author(s):  
Andrew T. Mariassy ◽  
Judith A. St. George ◽  
Susan J. Nishio ◽  
Charles G. Plopper
Keyword(s):  
Epithelial Cells ◽  
Tracheobronchial Epithelium

scholarly journals Biochemical and histochemical characterization of a murine tubular antigen.

1991 ◽  
Vol 1 (10) ◽  
pp. 1153-1161
Author(s):  
I D Davis ◽  
T W LeBien ◽  
B J Lindman ◽  
J L Platt
Keyword(s):  
Epithelial Cells ◽  
Tubular Epithelium ◽  
Ureteric Bud ◽  
Fetal Kidney ◽  
Cell Surface Antigens ◽  
Adenocarcinoma Cells ◽  
Nephron Development ◽  
Cellular Components ◽  
Histochemical Characterization

Cellular components of the developing fetal nephron express certain cell surface antigens at discrete stages of nephron development. To further evaluate the expression of antigens by tubular epithelium, a rat monoclonal antibody (BL26) was developed against murine fetal kidney. BL26 identified a differentiation antigen expressed by epithelial cells of the ureteric bud and late S-body in developing nephrons and by endothelium, mesangium, and distal tubular epithelium in mature nephrons. The polypeptide identified by BL26 was shown to be synthesized by fetal kidney and renal adenocarcinoma cells. The polypeptide contained no detectable carbohydrate modifications but was found in two isoforms, an acylated (26,000-Da) form and a nonacylated (24,000-Da) form. The murine antigen recognized by BL26 and the human CD9 moiety, an acylated polypeptide with a tissue distribution similar to that of the BL26 antigen, comigrated in polyacrylamide gels. We speculate that expression of the BL26 antigen and CD9 reflects processes relating to the activation of epithelial cells.

scholarly journals Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 139
Author(s):  
Johanna Detzner ◽  
Elisabeth Krojnewski ◽  
Gottfried Pohlentz ◽  
Daniel Steil ◽  
Hans-Ulrich Humpf ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Shiga Toxin ◽  
Saturated Fatty Acids ◽  
Human Kidney ◽  
Enterohemorrhagic Escherichia Coli ◽  
Highly Sensitive ◽  
Liquid Ordered ◽  
Uremic Syndrome

Human kidney epithelial cells are supposed to be directly involved in the pathogenesis of the hemolytic–uremic syndrome (HUS) caused by Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC). The characterization of the major and minor Stx-binding glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), respectively, of primary human renal cortical epithelial cells (pHRCEpiCs) revealed GSLs with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Using detergent-resistant membranes (DRMs) and non-DRMs, Gb3Cer and Gb4Cer prevailed in the DRM fractions, suggesting their association with microdomains in the liquid-ordered membrane phase. A preference of Gb3Cer and Gb4Cer endowed with C24:0 fatty acid accompanied by minor monounsaturated C24:1-harboring counterparts was observed in DRMs, whereas the C24:1 fatty acid increased in relation to the saturated equivalents in non-DRMs. A shift of the dominant phospholipid phosphatidylcholine with saturated fatty acids in the DRM to unsaturated species in the non-DRM fractions correlated with the GSL distribution. Cytotoxicity assays gave a moderate susceptibility of pHRCEpiCs to the Stx1a and Stx2a subtypes when compared to highly sensitive Vero-B4 cells. The results indicate that presence of Stx-binding GSLs per se and preferred occurrence in microdomains do not necessarily lead to a high cellular susceptibility towards Stx.

Characterization of liver epithelial cells transfected withmyc and/orras oncogenes

1991 ◽  
Vol 36 (5) ◽  
pp. 642-652 ◽  
Author(s):  
Stephen C. Strom ◽  
John B. Faust ◽  
Erika Cappelluti ◽  
Robert B. Harris ◽  
Narendra D. Lalwani
Keyword(s):  
Epithelial Cells ◽  
Liver Epithelial Cells
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