H+-K+-ATPase activity in the rat incisor enamel organ during enamel formation

1988 ◽  
Vol 221 (4) ◽  
pp. 823-833 ◽  
Author(s):  
Takahisa Sasaki ◽  
Katsumi Tadokoro ◽  
Takaaki Yanagisawa ◽  
Shohei Higashi ◽  
Philias R. Garant
Keyword(s):  
Atpase Activity ◽  
Enamel Organ ◽  
Enamel Formation ◽  
Rat Incisor

Penetration of various molecular-weight proteins into the enamel organ and enamel of the rat incisor

1986 ◽  
Vol 31 (5) ◽  
pp. 287-296 ◽  
Author(s):  
M.D. McKee ◽  
B. Martineau-Doize ◽  
H. Warshawsky
Keyword(s):  
Molecular Weight ◽  
Enamel Organ ◽  
Rat Incisor

MORPHOLOGIC AND FUNCTIONAL CORRELATIONS IN THE ENAMEL ORGAN OF THE RAT INCISOR DURING AMELOGENESIS

1959 ◽  
Vol 36 (4) ◽  
pp. 367-381 ◽  
Author(s):  
Jens J. Pindborg ◽  
Joseph P. Weinmann
Keyword(s):  
Enamel Organ ◽  
Rat Incisor

Identification of secreted and membrane proteins in the rat incisor enamel organ using a signal-trap screening approach

2006 ◽  
Vol 114 (s1) ◽  
pp. 139-146 ◽  
Author(s):  
Pierre Moffatt ◽  
Charles E. Smith ◽  
Roy Sooknanan ◽  
Rene St-Arnaud ◽  
Antonio Nanci
Keyword(s):  
Membrane Proteins ◽  
Enamel Organ ◽  
Rat Incisor ◽  
Screening Approach

scholarly journals A Study of the effect of 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) on the enamel formation in rat incisor

1990 ◽  
Vol 52 ◽  
pp. 301
Author(s):  
Hideaki Ogura ◽  
Hironari Wakamatsu ◽  
Keiichi Ohya ◽  
Shiro Mataki
Keyword(s):  
Enamel Formation ◽  
Rat Incisor

Effect of turpentine-induced fever during the enamel formation of rat incisor

2006 ◽  
Vol 51 (6) ◽  
pp. 464-470 ◽  
Author(s):  
Kuochung Tung ◽  
Haruko Fujita ◽  
Yasuo Yamashita ◽  
Yuzo Takagi
Keyword(s):  
Enamel Formation ◽  
Rat Incisor

Na-K-ATPase in the Enamel Organ: Localization and Possible Roles in Enamel Formation

1987 ◽  
Vol 1 (2) ◽  
pp. 267-275 ◽  
Author(s):  
P.R. Garant ◽  
T. Sasaki ◽  
P.E. Colflesh
Keyword(s):  
Plasma Membranes ◽  
Organic Matrix ◽  
Enamel Organ ◽  
Maturation Stage ◽  
Enamel Formation ◽  
Morphological Alterations ◽  
Maturation Stages ◽  
Delayed Maturation ◽  
The One

Ouabain-sensitive, K-dependent p-nitrophenyl phosphatase (p-NPPase) activity was localized ultra-Ocytochemically in the lateral plasma membranes of secretory ameloblasts and the stratum intermedium and principally in the papillary layer cells of aldehyde-fixed rat incisor enamel organs by the one-step lead method. Daily intraperitoneal injection of ouabain (250 μg, 500 μg, and 1 mg/100 g body weight) for two weeks reduced p-NPPase activity in the enamel organ cells. However, the degree to which this activity was reduced appeared to vary among the experimental animals. Addition of ouabain to the cytochemical incubation medium completely inhibited p-NPPase activity in the tissues. Although long-term ouabain injection did not result in any morphological alterations of the enamel organ cells, it caused, in part, an appearance of electron-dense, homogeneous matrix-like substances (MS) in the extracellular spaces of the ameloblast layers at both the secretion and maturation stages. In addition, long-term ouabain injection appeared to have resulted in delayed maturation of enamel as measured by energy-dispersive x-ray analysis of Ca and P in surface enamel. These results suggest that Na-K-ATPase of enamel organ cells may participate in the net flow (removal) of organic matrix components and water from the enamel during the maturation stage of enamel formation. It is suggested that this flow is maintained by local osmotic gradients generated by Na-K-ATPase within the papillary layer.

scholarly journals Localization of Transcriptional Co-activator CBP in the Ameloblasts and the Other Enamel Organ-derived Cells of the Rat Incisor

2002 ◽  
Vol 50 (11) ◽  
pp. 1455-1460 ◽  
Author(s):  
Sumio Nishikawa
Keyword(s):  
Molecular Weight ◽  
Transcriptional Regulation ◽  
Western Blotting ◽  
The Other ◽  
Enamel Organ ◽  
Creb Binding Protein ◽  
Rat Incisor ◽  
Specific Antibodies ◽  
Transition Zones ◽  
Western Blotting Analysis

CREB-binding protein (CBP) was examined in ameloblasts and in other enamel organ-derived cells of the rat incisor, using Western blotting analysis and immunocytochemistry by specific antibodies. Western blotting of labial tissues, including ameloblasts of the incisors, detected a single band with a molecular weight equivalent to the reported value of CBP. In immunocytochemistry, CBP was localized in ameloblast nuclei in the maturation zone but not in the secretion and transition zones. The nuclei of the other enamel organ-derived cells were also positive. Because this protein is suggested to take part in c-Jun-mediated transcription, the present study and the results of a previous report showing c-Jun localization in the nuclei of enamel organ-derived cells suggest that the enamel organ-derived cells, including maturation ameloblasts, undergo active transcriptional regulation.

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