Influence of colchicine on the distribution of horseradish peroxidase in the secretory ameloblast layer in vitro

1986 ◽  
Vol 216 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Saburou Matsuo ◽  
Katsuhiko Yamamoto ◽  
Shinji Nishikawa ◽  
Hiroyuki Ichikawa ◽  
Satoshi Wakisaka ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Secretory Ameloblast

The distribution of ingested horseradish peroxidase in the 16-cell mouse embryo

Development ◽  
1981 ◽  
Vol 66 (1) ◽  
pp. 191-207
Author(s):  
W. J. D. Reeve
Keyword(s):  
Horseradish Peroxidase ◽  
Mouse Embryo ◽  
Single Cells ◽  
Fluorescent Antibody ◽  
Surface Binding ◽  
Cytoplasmic Organization ◽  
Cell Embryo ◽  
Polarized Surface ◽  
Cell Mouse Embryo

Cells of the 16-cell mouse embryo endocytose horseradish peroxidase (HRP) which becomes localized in most cases to a juxtanuclear position. Cells that have ingested HRP in intact embryos, and cells dissociated from embryos prior to culture in HRP, showed similar patterns of cytoplasmic distribution of the ingested enzyme. Cells in the embryo in situ were incubated in HRP, and then labelled with fluorescent antibody either before (to label the outside surface of the embryo) or after (to reveal populations of outer polar and inner apolar cells) their disaggregation into single cells. The population of polar outside cells from the morula includes more cells with a highly restricted localization of HRPcontaining vesicles than does the population of inside cells, and this restricted localization underlies the exposed surface or pole of the cell. A 2/16 couplet formed by division in vitro of a 1/8 cell is comparable to the pairs of cells dissociated from 16-cell embryos; most couplets from either source consisted of a larger cell that showed polarized surface binding of fluorescent ligand (fluorescent pole) and a smaller cell with a uniform distribution of bound ligand. The incidence of restricted patterns of HRP staining was highest among populations of both larger and polar cells. When 1/8 cells labelled with HRP are observed during division to 2/16, the previously clustered vesicles of ingested HRP become more dispersed throughout the cytoplasm and, although the two cells of some couplets can stain differently very soon after their formation, the patterns of distribution of HRP take about 1 h after division to stabilize. These observations are consistent with cells of the 16-cell embryo inheriting different features of cytoplasmic organization.

Effects of Chondroitin Sulphate on Fluid and Solute Transport during Peritoneal Dialysis in Rats

1991 ◽  
Vol 11 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Andrzej Breborowicz ◽  
Maciej Radkowski ◽  
Jan Knapowski ◽  
Dimitrios G. Oreopoulos
Keyword(s):  
Peritoneal Dialysis ◽  
Hydrostatic Pressure ◽  
Hydraulic Conductivity ◽  
Horseradish Peroxidase ◽  
Solute Transport ◽  
Peritoneal Cavity ◽  
Chondroitin Sulphate ◽  
In Vitro Experiments ◽  
Fluid Removal

The effect of chondroitin sulphate (CS) on peritoneal fluid and solute transport was studied in rats undergoing peritoneal dialysis. In the presence of CS, net ultrafiltration increased, while absorption of glucose and horseradish peroxidase from the peritoneal cavity decreased. Albumin, used instead of CS, did not modify either fluid or solute transport. In in vitro experiments on isolated rabbit mesentery, CS decreased transmembrane water flow induced by hydrostatic pressure, and its effect was not fully reversed 60 minutes after “wash-out” of this glycosaminoglycan. We postulate that the polyanionic CS molecules are trapped in the peritoneal interstitium, thus decreasing its hydraulic conductivity and permeability, which in turn increases net fluid removal during peritoneal dialy sis because of its slower absorption from the peritoneal cavity.

scholarly journals LOCALIZATION OF THE ANTIGEN IN POPLITEAL LYMPH NODES OF RABBITS DURING THE FORMATION OF ANTIBODIES TO HORSERADISH PEROXIDASE

1970 ◽  
Vol 18 (2) ◽  
pp. 131-142 ◽  
Author(s):  
WERNER STRAUS
Keyword(s):  
Horseradish Peroxidase ◽  
Lymph Nodes ◽  
Specific Antibody ◽  
Plasma Cells ◽  
Repeated Injection ◽  
Before And After ◽  
Reticular Cells ◽  
Popliteal Lymph Nodes ◽  
Tissue Material

The localization of an antigen (horseradish peroxidase) in popliteal lymph nodes of rabbits was investigated in order to detect the possible interrelationship with the location of the specific antibody in the same tissue material. Staining procedures for peroxidase with benzidine, diaminobenzidine and 3-amino-9-ethyl-carbazole, as well as double staining procedures for the antigen and the antibody and for the antigen (or antibody) and acid phosphatase, were applied before and after adsorption of the antigen to sites of antibody in vitro. The appearance of the antigen in the cells lining the lymph sinuses, in reticular cells of medullary cords, in macrophages and in the "intercellular web" of lymphoid follicles was studied after a single and repeated injection of peroxidase, and the persistence of the antigen at these sites was observed. It was found that the localization of the antigen in the cortex and medulla of the lymph node was different depending on whether or not specific antibodies were present in the blood at the time of injection, and that at certain periods a considerable number of plasma cells and lymphoblasts contained the antigen together with the specific antibody.

Studies on the dehydrogenative polymerization of monolignol β-glycosides. Part 6: Monitoring of horseradish peroxidase-catalyzed polymerization of monolignol glycosides by GPC-PDA

Holzforschung ◽  
2010 ◽  
Vol 64 (2) ◽  
Author(s):  
Yuki Tobimatsu ◽  
Toshiyuki Takano ◽  
Hiroshi Kamitakahara ◽  
Fumiaki Nakatsubo
Keyword(s):  
Horseradish Peroxidase ◽  
Reaction System ◽  
Water Soluble ◽  
Quinone Methide ◽  
Efficient Production ◽  
Hydroxyl Groups ◽  
Photodiode Array Detection ◽  
Dehydrogenative Polymerization

Abstract Horseradish peroxidase (HRP)-catalyzed dehydrogenative polymerization of guaiacyl (G) and syringyl (S)-type monolignol γ-O-glucosides, isoconiferin (iso-G) and isosyringin (iso-S), which contain a hydrophilic glucosyl unit on γ-position of coniferyl alcohol and sinapyl alcohol, respectively, was monitored by gel permeation chromatography coupled with photodiode array detection (GPC-PDA). Contrary to the conventional dehydrogenative polymerization of monolignols, the polymerization of the glycosides produces water-soluble synthetic lignins (DHPs) in a homogeneous aqueous phase. Taking advantage of this unique reaction system, the method was developed to follow the changes of molecular weights in the course of DHP formations. Moreover, PDA detection permits determination of oligomeric S-type quinone methide intermediates (QMs) formed as stable transient compounds during polymerization of iso-S. A detailed comparison of the polymerization profiles revealed entirely different behaviors of G- and S-type monomers. The data strongly support the view that the low reactivity of oligomeric S-type QMs impedes the formation of DHPs from S-type monomers. In copolymerization of G- and S-type monomers, it is conceivable that G-type phenolic hydroxyl groups serve as good nucleophilic reactants to scavenge S-type QMs resulting in efficient production of DHPs. As a consequence, the present approach can be a powerful tool to study the in vitro dehydrogenative polymerization providing further mechanistic insights into lignin polymerization in vivo.

Sign in / Sign up

Export Citation Format

Share Document