Surface area ratios. I. A stereological method for estimating average cell changes in membrane surface areas

1979 ◽  
Vol 194 (4) ◽  
pp. 511-522 ◽  
Author(s):  
Robert P. Bolender
Keyword(s):  
Surface Area ◽  
Membrane Surface ◽  
Average Cell ◽  
Stereological Method ◽  
Surface Areas ◽  
Cell Changes

A stereological comparison of villous and microvillous surfaces in small intestines of frugivorous and entomophagous bats: species, inter-individual and craniocaudal differences.

1997 ◽  
Vol 200 (18) ◽  
pp. 2415-2423 ◽  
Author(s):  
A N Makanya ◽  
J N Maina ◽  
T M Mayhew ◽  
S A Tschanz ◽  
P H Burri
Keyword(s):  
Surface Area ◽  
Body Mass ◽  
Membrane Surface ◽  
Total Surface Area ◽  
Membrane Surface Area ◽  
Intestinal Tube ◽  
Surface Areas ◽  
Intestinal Length ◽  
Mass Coefficient ◽  
Extensive Surface

The extents of functional surfaces (villi, microvilli) have been estimated at different longitudinal sites, and in the entire small intestine, for three species of bats belonging to two feeding groups: insect- and fruit-eaters. In all species, surface areas and other structural quantities tended to be greatest at more cranial sites and to decline caudally. The entomophagous bat (Miniopterus inflatus) had a mean body mass (coefficient of variation) of 8.9 g (5%) and a mean intestinal length of 20 cm (6%). The surface area of the basic intestinal tube (primary mucosa) was 9.1 cm2 (10%) but this was amplified to 48 cm2 (13%) by villi and to 0.13 m2 (20%) by microvilli. The total number of microvilli per intestine was 4 x 10(11) (20%). The average microvillus had a diameter of 8 nm (10%), a length of 1.1 microns (22%) and a membrane surface area of 0.32 micron 2 (31%). In two species of fruit bats (Epomophorus wahlbergi and Lisonycteris angolensis), body masses were greater and intestines longer, the values being 76.0 g (18%) and 76.9 g (4%), and 73 cm (16%) and 72 cm (7%), respectively. Surface areas were also greater, amounting to 76 cm2 (26%) and 45 cm2 (8%) for the primary mucosa, 547 cm2 (29%) and 314 cm2 (16%) for villi and 2.7 m2 (23%) and 1.5 m2 (18%) for microvilli. An increase in the number of microvilli, 33 x 10(11) (19%) and 15 x 10(11) (24%) per intestine, contributed to the more extensive surface area but there were concomitant changes in the dimensions of microvilli. Mean diameters were 94 nm (8%) and 111 nm (4%), and mean lengths were 2.8 microns (12%) and 2.9 microns (10%), respectively. Thus, an increase in the surface area of the average microvillus to 0.83 micron 2 (12%) and 1.02 microns 2 (11%) also contributed to the greater total surface area of microvilli. The lifestyle-related differences in total microvillous surface areas persisted when structural quantities were normalised for the differences in body masses. The values for total microvillous surface area were 148 cm2g-1 (20%) in the entomophagous bat, 355 cm2g-1 (20%) in E. wahlbergi and 192 cm2g-1 (17%) in L. angolensis. This was true despite the fact that the insecteater possessed a greater length of intestine per unit of body mass: 22 mm g-1 (8%) versus 9-10 mm g-1 (9-10%) for the fruit-eaters.

scholarly journals Effect of Acid Flow Rate, Membrane Surface Area, and Capture Solution on the Effectiveness of Suspended GPM Systems to Recover Ammonia

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 538
Author(s):  
María Soto-Herranz ◽  
Mercedes Sánchez-Báscones ◽  
Juan Manuel Antolín-Rodríguez ◽  
Matías B. Vanotti ◽  
Pablo Martín-Ramos
Keyword(s):  
Surface Area ◽  
Flow Rate ◽  
Membrane Surface ◽  
Animal Health ◽  
Limiting Factors ◽  
Membrane Surface Area ◽  
Surface Areas ◽  
N Concentration ◽  
Chilled Water ◽  
Ammonia Recovery

Ammonia losses from manure pose serious problems for ecosystems and human and animal health. Gas-permeable membranes (GPMs) constitute a promising approach to address the challenge of reducing farm ammonia emissions and to attain the EU’s Clean Air Package goals. In this study, the effect of NH3-N concentration, membrane surface area, acid flux, and type of capture solution on ammonia recovery was investigated for a suspended GPM system through three experiments, in which ammonia was released from a synthetic solution (NH4Cl + NaHCO3 + allylthiourea). The effect of two surface areas (81.7 and 163.4 cm2) was first evaluated using three different synthetic N emitting concentrations (3000, 6000, and 12,000 mg NH3-N∙L−1) and keeping the flow of acidic solution (1N H2SO4) constant (0.8 L·h−1). A direct relationship was found between the amount of NH3 captured and the NH3-N concentration in the N-emitting solution, and between the amount of NH3 captured and the membrane surface area at the two lowest concentrations. Nonetheless, the use of a larger membrane surface barely improved ammonia capture at the highest concentration, pointing to the existence of other limiting factors. Hence, ammonia capture was then studied using different acid flow rates (0.8, 1.3, 1.6, and 2.1 L∙h−1) at a fixed N emitting concentration of 6000 mg NH3-N∙L−1 and a surface area of 122.5 cm2. A higher acid flow rate (0.8–2.1 L∙h−1) resulted in a substantial increase in ammonia absorption, from 165 to 262 mg of NH3∙d−1 over a 14-day period. Taking the parameters that led to the best results in experiments 1 and 2, different types of ammonia capture solutions (H2SO4, water and carbonated water) were finally compared under refrigeration conditions (at 2 °C). A high NH3 recovery (81% in 7 days), comparable to that obtained with the H2SO4 solution (88%), was attained when chilled water was used as the capture solution. The presented results point to the need to carefully optimize the emitter concentration, flow rate, and type of capture solution to maximize the effectiveness of suspended GPM systems, and suggest that chilled water may be used as an alternative to conventional acidic solutions, with associated savings.

Ultrastructural morphometric of gap junctions during differentiation of stratified squamous epithelium

1984 ◽  
Vol 69 (1) ◽  
pp. 67-85
Author(s):  
F.H. White ◽  
D.A. Thompson ◽  
K. Gohari
Keyword(s):  
Plasma Membrane ◽  
Gap Junctions ◽  
Surface Area ◽  
Present Report ◽  
Membrane Surface ◽  
Granular Cell ◽  
Cheek Pouch ◽  
Hamster Cheek Pouch ◽  
Surface Areas ◽  
Stratified Epithelia

The presence of gap junctions in stratified epithelia has now been extensively documented, but there have been few attempts to quantify them. In the present report, samples of hamster cheek pouch mucosa were processed for electron microscopy and electron micrographs from defined basal, spinous and granular layers were obtained. Using a combination of direct measurement and stereological intersection counting techniques, the relative surface areas of peripheral gap junctions (i.e. those in direct contact with the epithelial plasma membrane) and annular gap junctions (i.e. those present as complete, approximately circular profiles within the epithelial cell cytoplasm) were determined. Following estimation of the plasma membrane surface area of ‘average’ epithelial cells from each of the defined strata, relative values were transformed into absolute data. Data from peripheral and annular junctions were pooled to provide an estimate of total gap junctions area. Relative surface area estimates were similar for peripheral, annular and total gap junctions, in that values were invariably highest in the spinous layer and lowest in the granular layer. Absolute data indicate that there is more than a threefold increase in the area of membrane differentiated into gap junctions in the average spinous cell when compared with the average basal cell. Values for total gap-junctional areas in the average granular cell are reduced somewhat with respect to the average spinous cell and this is effected by a decrease in the area of peripheral gap junctions. We conclude that there is synthesis of gap junctions between basal and spinous cells, which is followed by evidence of degradation between spinous and granular cells. The magnitude of the estimates of area is comparable to those obtained from other stratified and non-stratified epithelia and it would thus appear that gap junctions may play a significant role in cellular control processes in all viable epithelial strata.

Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

1987 ◽  
Vol 45 ◽  
pp. 588-589
Author(s):  
M. Marko ◽  
A. Leith ◽  
D. Parsons
Keyword(s):  
Surface Area ◽  
Electron Micrographs ◽  
Cell Morphology ◽  
Individual Variation ◽  
Serial Section ◽  
Stereo Pair ◽  
Complex Structures ◽  
Serial Sections ◽  
Surface Areas ◽  
Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

Hair cell changes after cationic stimulation of corti's organ

1989 ◽  
Vol 47 ◽  
pp. 798-799
Author(s):  
Cesar D. Fermin ◽  
Hans-Peter Zenner
Keyword(s):  
Organ Of Corti ◽  
Cross Sectional ◽  
Surface Areas ◽  
High K ◽  
Anatomical Arrangement ◽  
Cell Cytosol ◽  
High Concentrations ◽  
K Concentration ◽  
Cell Changes

Contraction of outer and inner hair cells (OHC&IHC) in the Organ of Corti (OC) of the inner ear is necessary for sound transduction. Getting at HC in vivo preparations is difficult. Thus, isolated HCs have been used to study OHC properties. Even though viability has been shown in isolated (iOHC) preparations by good responses to current and cationic stimulation, the contribution of adjoining cells can not be explained with iOHC preparations. This study was undertaken to examine changes in the OHC after expossure of the OHC to high concentrations of potassium (K) and sodium (Na), by carefully immersing the OC in either artifical endolymph or perilymph. After K and Na exposure, OCs were fixed with 3% glutaraldehyde, post-fixed in osmium, separated into base, middle and apex and embedded in Araldite™. One μm thick sections were prepared for analysis with the light and E.M. Cross sectional areas were measured with Bioquant™ software.Potassium and sodium both cause isolated guinea pig OHC to contract. In vivo high K concentration may cause uncontrolled and sustained contractions that could contribute to Meniere's disease. The behavior of OHC in the vivo setting might be very different from that of iOHC. We show here changes of the cell cytosol and cisterns caused by K and Na to OHC in situs. The table below shows results from cross sectional area measurements of OHC from OC that were exposed to either K or Na. As one would expect, from the anatomical arrangement of the OC, OHC#l that are supported by rigid tissue would probably be displaced (move) less than those OHC located away from the pillar. Surprisingly, cells in the middle turn of the cochlea changed their surface areas more than those at either end of the cochlea. Moreover, changes in surface area do not seem to differ between K and Na treated OCs.

scholarly journals Tuning the hierarchical pore structure of graphene oxide through dual thermal activation for high-performance supercapacitor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeongpil Kim ◽  
Jeong-Hyun Eum ◽  
Junhyeok Kang ◽  
Ohchan Kwon ◽  
Hansung Kim ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Pore Structure ◽  
Specific Capacitance ◽  
Surface Area ◽  
Thermal Annealing ◽  
High Performance ◽  
Annealing Process ◽  
Supercapacitor Electrode ◽  
Simple Method ◽  
Surface Areas

AbstractHerein, we introduce a simple method to prepare hierarchical graphene with a tunable pore structure by activating graphene oxide (GO) with a two-step thermal annealing process. First, GO was treated at 600 °C by rapid thermal annealing in air, followed by subsequent thermal annealing in N2. The prepared graphene powder comprised abundant slit nanopores and micropores, showing a large specific surface area of 653.2 m2/g with a microporous surface area of 367.2 m2/g under optimized conditions. The pore structure was easily tunable by controlling the oxidation degree of GO and by the second annealing process. When the graphene powder was used as the supercapacitor electrode, a specific capacitance of 372.1 F/g was achieved at 0.5 A/g in 1 M H2SO4 electrolyte, which is a significantly enhanced value compared to that obtained using activated carbon and commercial reduced GO. The performance of the supercapacitor was highly stable, showing 103.8% retention of specific capacitance after 10,000 cycles at 10 A/g. The influence of pore structure on the supercapacitor performance was systematically investigated by varying the ratio of micro- and external surface areas of graphene.

The macrophage capacity for phagocytosis

1992 ◽  
Vol 101 (4) ◽  
pp. 907-913 ◽  
Author(s):  
G.J. Cannon ◽  
J.A. Swanson
Keyword(s):  
Surface Area ◽  
Membrane Surface ◽  
Fc Receptors ◽  
Phagocytic Capacity ◽  
Membrane Surface Area ◽  
Murine Bone Marrow ◽  
Latex Beads ◽  
Endocytic Compartments ◽  
Frustrated Phagocytosis

Murine bone marrow-derived macrophages, which measure 13.8 +/− 2.3 microns diameter in suspension, can ingest IgG-opsonized latex beads greater than 20 microns diameter. A precise assay has allowed the determination of the phagocytic capacity, and of physiological parameters that limit that capacity. Ingestion of beads larger than 15 microns diameter required IgG-opsonization, and took 30 minutes to reach completion. Despite the dependence on Fc-receptors for phagocytosis of larger beads, cells reached their limit before all cell surface Fc-receptors were occupied. The maximal membrane surface area after frustrated phagocytosis of opsonized coverslips was similar to the membrane surface area required to engulf particles at the limiting diameter, indicating that the capacity was independent of particle shape. Vacuolation of the lysosomal compartment with sucrose, which expanded endocytic compartments, lowered the phagocytic capacity. This decrease was reversed when sucrose vacuoles were collapsed by incubation of cells with invertase. These experiments indicate that the phagocytic capacity is limited by the amount of available membrane, rather than by the availability of Fc-receptors. The capacity was also reduced by depolymerization of cytoplasmic microtubules with nocodazole. Nocodazole did not affect the area of maximal cell spreading during frustrated phagocytosis, but did alter the shape of the spread cells. Thus, microtubules may coordinate cytoplasm for engulfment of the largest particles.

The new face of large dams in the 21st century

2021 ◽  
Author(s):  
Atal Ahmadzai
Keyword(s):  
Surface Area ◽  
Aquatic Ecosystems ◽  
Energy Demand ◽  
Environmental Aspects ◽  
Water Insecurity ◽  
Surface Areas ◽  
Large Dams ◽  
Physical Attributes ◽  
Under Construction ◽  
Ecological Disturbances

Alerted by increasing water insecurity and energy demand, countries, mainly in the Global South, are building dams of unprecedented magnitude. Hundreds of large dams (≥ 100 metres) have been constructed since 2000, with hundreds more under construction. Analyses of the physical attributes of these dams present a concerning image. While they create expansive reservoirs with large surface areas, they have inefficient surface area-to-volume ratios ('S2VR'). Their unprecedented size and the reservoirs’ expansive surface area, indicate severe environmental costs, mainly through ecological disturbances to the (riverine) aquatic ecosystems; and greenhouse gas emissions (GHG). Other ecological costs due to the larger S2VR include a high evaporation rate and compromised biodiversity of a wider area, both up- and downstream. The safety and environmental aspects of these large dams should be robustly scrutinised.

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