Secretagogue-mediated discharge of nerve growth factor from granular tubules of male mouse submandibular glands: An immunocytochemical study

1978 ◽  
Vol 192 (3) ◽  
pp. 375-387 ◽  
Author(s):  
J. A. V. Simson ◽  
D. Hazen ◽  
S. S. Spicer ◽  
R. A. Murphy ◽  
M. Young
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Male Mouse ◽  
Immunocytochemical Study ◽  
Nerve Growth ◽  
Submandibular Glands

scholarly journals Detection of nerve growth factor mRNA in rodent salivary glands with digoxigenin- and 33P-labeled oligonucleotides: effects of castration and sympathectomy.

1993 ◽  
Vol 41 (5) ◽  
pp. 703-708 ◽  
Author(s):  
C Humpel ◽  
E Lindqvist ◽  
L Olson
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Male Mouse ◽  
Male Mice ◽  
Nerve Growth ◽  
Submandibular Glands

Nerve growth factor (NGF) is a protein highly expressed in the male mouse submandibular gland. We have applied a non-radioactive in situ hybridization method using digoxigenin-labeled NGF oligonucleotides, and have found the highest amounts of NGF mRNA in the secretory striated ducts of the male mouse submandibular gland. Scattered strongly positive cells were found in male mouse sublingual glands. Weakly labeled cells were seen in female mouse and in male rat submandibular gland striated duct cells. Using 33P as an alternative to 32P and 35S, we demonstrated a 1.3 KB NGF mRNA in salivary glands of male mice by Northern blot hybridization. Using 33P we detected NGF mRNA in male mouse submandibular glands by in situ hybridization but with a signal that, compared with the non-radioactive method, had a very low resolution. Castration of male mice almost abolished both the 1.3 KB NGF mRNA seen with Northern blots and the NGF mRNA labeling in submandibular glands 4 weeks after the operation, whereas levels were increased 6 hr and 2 days after sympathectomy. We conclude that hybridization with digoxigenin-labeled NGF oligonucleotides is a good tool to study the expression and regulation of NGF mRNA in male mouse submandibular glands.

Submandibular glands in mice with muscular dystrophy: Studies with nerve growth factor

1981 ◽  
Vol 200 (2) ◽  
pp. 177-194 ◽  
Author(s):  
Richard A. Murphy ◽  
Ann Y. Watson ◽  
Mary McCarthy ◽  
Mary Papastavros ◽  
Marian Neutra ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Muscular Dystrophy ◽  
Nerve Growth ◽  
Submandibular Glands

scholarly journals Isolation and Characterization of a Glycosylated Form of β Nerve Growth Factor in Mouse Submandibular Glands

1989 ◽  
Vol 264 (21) ◽  
pp. 12502-12509 ◽  
Author(s):  
R A Murphy ◽  
V Chlumecky ◽  
L B Smillie ◽  
M Carpenter ◽  
M Nattriss ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Isolation And Characterization

Cellular and subcellular colocalization of nerve growth factor and epidermal growth factor in mouse submandibular glands

1985 ◽  
Vol 213 (3) ◽  
pp. 365-376 ◽  
Author(s):  
Ann Y. Watson ◽  
Jacqueline K. Anderson ◽  
Kerry Siminoski ◽  
John E. Mole ◽  
Richard A. Murphy
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Epidermal Growth

Comparison of 7S nerve growth factor and nerve growth factor I from mouse submandibular glands

Biochemistry ◽  
1986 ◽  
Vol 25 (4) ◽  
pp. 754-760 ◽  
Author(s):  
Nicholas G. Guerina ◽  
Nicholas J. Pantazis ◽  
Kerry Siminoski ◽  
Jacqueline K. Anderson ◽  
Mary McCarthy ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Factor I ◽  
Growth Factor I
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