Intercellular junctional complexes of the rat seminiferous tubules: A freeze-fracture study

1977 ◽  
Vol 189 (2) ◽  
pp. 211-231 ◽  
Author(s):  
Dennis McGinley ◽  
Zoltan Posalaky ◽  
Martin Porvaznik
Keyword(s):  
Freeze Fracture ◽  
Seminiferous Tubules ◽  
Fracture Study ◽  
Junctional Complexes

Perineurium of sciatic nerve in normal and diabetic rodents: freeze-fracture study of intercellular junctional complexes

1991 ◽  
Vol 20 (6) ◽  
pp. 459-470 ◽  
Author(s):  
A. Schiavinato ◽  
A. R. Morandin ◽  
D. Guidolin ◽  
E. Lini ◽  
M. G. Nunzi ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Freeze Fracture ◽  
Fracture Study ◽  
Junctional Complexes

Formation of Junctional Complexes in Otocysts Developed in VitroA Freeze fracture Study

1987 ◽  
Vol 104 (1-2) ◽  
pp. 146-152 ◽  
Author(s):  
Diana Berggren ◽  
Dan Bagger-sjöbäck ◽  
Matti Anniko
Keyword(s):  
Freeze Fracture ◽  
Fracture Study ◽  
Junctional Complexes

Freeze-Fracture Examination of Tight Junctions of Human Eccrine Sweat Glands

1980 ◽  
Vol 38 ◽  
pp. 634-635
Author(s):  
J. V. Briggman ◽  
J. Bigelow ◽  
H. Bank ◽  
S. S. Spicer
Keyword(s):  
Cystic Fibrosis ◽  
Freeze Fracture ◽  
Sweat Glands ◽  
Hypertonic Solution ◽  
Dark Cells ◽  
Clear Cells ◽  
Junctional Complexes ◽  
Secretory Coil ◽  
Eccrine Sweat Glands ◽  
Eccrine Sweat

The prevalence of strands shown by freeze-fracture in the zonula occludens of junctional complexes is thought to correspond closely with the transepi-thelial electrical resistance and with the tightness of the junction and its obstruction to paracellular flow.1 The complexity of the network of junc¬tional complex strands does not appear invariably related to the degree of tightness of the junction, however, as rabbit ileal junctions have a complex network of strands and are permeable to lanthanum. In human eccrine sweat glands the extent of paracellular relative to transcellular flow remains unknown, both for secretion of the isotonic precursor fluid by the coil and for resorption of a hypertonic solution by the duct. The studies reported here undertook, therefore, to determine with the freeze-fracture technique the complexity of the network of ridges in the junctional complexes between cells in the secretory coil and the sweat ducts. Glands from a patient with cystic fibrosis were also examined because an alteration in junctional strands could underlie the decreased Na+ resorption by sweat ducts in this disease. Freeze-fracture replicas were prepared by standard procedures on isolated coil and duct segments of human sweat glands. Junctional complexes between clear cells, between dark cells and between clear and dark cells on the main lumen, and between clear cells on intercellular canaliculi of the coil con¬tained abundant anastomosing closely spaced strands averaging 6.4 + 0.7 (mean + SE) and 9.0 +0.5 (Fig. 1) per complex, respectively. Thus, the junctions in the intercellular canaliculi of the coil appeared comparable in complexity to those of tight epithlia. Occasional junctions exhibited, in addition, 2 to 5 widely spaced anastomosing strands in a very close network basal to the compact network. The fewer junctional complexes observed thus far between the superficial duct cells consisted on the average of 6 strands arranged in a close network and 1 to 4 underlying strands that lay widely separated from one another (Fig. 2). The duct epitelium would, thus, be judged slightly more "leaky" than the coil. Infrequent junctional complexes observed to date in the secretory coil segment of a cystic fibrosis specimen disclosed rela¬tively few closely crowded strands.

Junctional Pattern in the Squamous Metaplasia of the Female Trigone. A Freeze-Fracture Study

1983 ◽  
Vol 129 (2) ◽  
pp. 280-283 ◽  
Author(s):  
M. Sideri ◽  
G. De Virgiliis ◽  
R. Rainoldi ◽  
A. Ferrari ◽  
G. Remotti
Keyword(s):  
Squamous Metaplasia ◽  
Freeze Fracture ◽  
Fracture Study

Freeze-fracture study of the mechanoreceptive digital corpuscles of mice

1985 ◽  
Vol 14 (6) ◽  
pp. 1037-1052 ◽  
Author(s):  
Chizuka Ide ◽  
Kenichi Kumagai ◽  
Schuichiro Hayashi
Keyword(s):  
Freeze Fracture ◽  
Fracture Study
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