A case of anal atresia and female pseudohermaphrodism in the fetal pig

1959 ◽  
Vol 133 (1) ◽  
pp. 13-17
Author(s):  
Daniel T. Magidson
Keyword(s):  
Anal Atresia ◽  
Fetal Pig

scholarly journals Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species

2021 ◽  
Vol 22 (14) ◽  
pp. 7589
Author(s):  
Anberitha T. Matthews ◽  
Hitesh Soni ◽  
Katherine E. Robinson-Freeman ◽  
Theresa A. John ◽  
Randal K. Buddington ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Transient Receptor Potential ◽  
Mesangial Cell ◽  
Reactive Oxygen ◽  
Receptor Potential ◽  
Oxygen Species ◽  
Glomerular Mesangial Cell ◽  
Trpc6 Channel ◽  
Fetal Pig

Doxorubicin (DOX), a category D pregnancy drug, is a chemotherapeutic agent that has been shown in animal studies to induce fetal toxicity, including renal abnormalities. Upregulation of the transient receptor potential cation (TRPC) 6 channel is involved in DOX-induced podocyte apoptosis. We have previously reported that TRPC6-mediated Ca2+ signaling promotes neonatal glomerular mesangial cell (GMC) death. However, it is unknown whether DOX alters mesangial TRPC expression or viability in the fetus. In this study, cell growth was tracked in control and DOX-treated primary GMCs derived from fetal pigs. Live-cell imaging demonstrated that exposure to DOX inhibited the proliferation of fetal pig GMCs and induced cell death. DOX did not alter the TRPC3 expression levels. By contrast, TRPC6 protein expression in the cells was markedly reduced by DOX. DOX treatment also attenuated the TRPC6-mediated intracellular Ca2+ elevation. DOX stimulated mitochondrial reactive oxygen species (mtROS) generation and mitophagy by the GMCs. The DOX-induced mtROS generation and apoptosis were reversed by the mitochondria-targeted antioxidant mitoquinone. These data suggest that DOX-induced fetal pig GMC apoptosis is independent of TRPC6 channel upregulation but requires mtROS production. The mtROS-dependent GMC death may contribute to DOX-induced fetal nephrotoxicity when administered prenatally.

KV7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure

2022 ◽  
Author(s):  
Dieniffer Peixoto-Neves ◽  
Praghalathan Kanthakumar ◽  
Jeremiah M Afolabi ◽  
Hitesh Soni ◽  
Randal K Buddington ◽  
...  
Keyword(s):  
Arterial Pressure ◽  
Functional Expression ◽  
Renal Autoregulation ◽  
Neonatal Pigs ◽  
Vascular Smcs ◽  
Neonatal Pig ◽  
Kidney Maturation ◽  
Renal Vascular ◽  
Renal Vascular Tone ◽  
Fetal Pig

KV7, the voltage-gated potassium channels encoded by KCNQ genes, mediate heterogeneous vascular responses in adult rodents. Postnatal changes in the functional expression of KV7 channels have been reported in rodent saphenous arteries, but their physiological function in the neonatal renal vascular bed is unclear. Here, we report that, unlike adult pigs, only KCNQ1 (KV7.1) out of the five members of KCNQ genes was detected in neonatal pig renal microvessels. KCNQ1 is present in fetal pig kidneys as early as day 50 of gestation, and the level of expression remains the same up to postnatal day 21. Activation of the renal vascular smooth muscle cell (SMC) KV7.1 stimulated whole-cell currents, inhibited by HMR1556 (HMR), a selective KV7.1 blocker. HMR did not change the steady-state diameter of isolated renal microvessels. Similarly, intrarenal artery infusion of HMR did not alter the mean arterial pressure (MAP), renal blood flow (RBF), and renal vascular resistance (RVR) in the pigs. An approximately 20 mmHg reduction in the MAP evoked effective autoregulation of the RBF, which HMR inhibited. We conclude that 1) The expression of KCNQ isoforms in porcine renal microvessels is dependent on kidney maturation, 2) KV7.1 is functionally expressed in neonatal pig renal vascular SMCs, 3) a decrease in arterial pressure up to 20 mmHg induces renal autoregulation in neonatal pigs, and 4) SMC KV7.1 does not control basal renal vascular tone but contributes to neonatal renal autoregulation triggered by a step decrease in arterial pressure.

Distribution between Protein-Bound and Free Forms of Plasma Cortisol in the Gilt and Fetal Pig Near Term

Neonatology ◽  
1997 ◽  
Vol 72 (3) ◽  
pp. 192-200 ◽  
Author(s):  
H.G. Kattesh ◽  
G.A. Baumbach ◽  
B.B. Gillespie ◽  
J.F. Schneider ◽  
J.T. Murai
Keyword(s):  
Plasma Cortisol ◽  
Near Term ◽  
Fetal Pig

scholarly journals Characterisation of the Xenogeneic Immune Response to Microencapsulated Fetal Pig Islet-Like Cell Clusters Transplanted into Immunocompetent C57BL/6 Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59120 ◽  
Author(s):  
Vijayaganapathy Vaithilingam ◽  
Cherry Fung ◽  
Sabina Ratnapala ◽  
Jayne Foster ◽  
Vijesh Vaghjiani ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Clusters ◽  
Fetal Pig

scholarly journals Proximal 10q trisomy: a new case with anal atresia

2000 ◽  
Vol 37 (9) ◽  
pp. 24e-24 ◽  
Author(s):  
F W F LAM
Keyword(s):  
Anal Atresia

scholarly journals A Very Rare Cause of Anal Atresia: Currarino Syndrome

2016 ◽  
Vol 8 (5) ◽  
pp. 420-423 ◽  
Author(s):  
Sevgi Buyukbese Sarsu ◽  
Mehmet Ergun Parmaksiz ◽  
Esra Cabalar ◽  
Ali Karapur ◽  
Cihat Kaya
Keyword(s):  
Anal Atresia ◽  
Currarino Syndrome
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