Cyclic changes in the pituitary gland of the urodele amphibian, Taricha torosa (Triturus torosus)

1955 ◽  
Vol 122 (1) ◽  
pp. 105-114 ◽  
Author(s):  
Malcolm R. Miller ◽  
Marilyn E. Robbins
1996 ◽  
Vol 7 (1) ◽  
pp. 271-284
Author(s):  
M. ZAKI ◽  
A. MASSOUD ◽  
G. MADKOUR ◽  
N. EL-FIKY ◽  
J. EL-MESIRY

2013 ◽  
Vol 1 (02) ◽  
pp. 12-19
Author(s):  
Z. B. Khuroo

The presence of functional hypothalamo-hypophysial neurosecretory system in vertebrates has led to revise earlier views regarding the hypothalamus-pituitary relationship. The hypothalamo-neurohypophysial system which consists of hypothalamic nuclei, their axonal fibres forming tractus preoptico-hypophyses and the neurohypophysis) works as a morphological as well as a physiological connection between the hypothalamus and the pituitary gland. In fishes, fibres from the neurosecretory hypothalamic nuclei terminate in the neurohypophysis, which remains inter digitized with the adenohypophysis and provides a very close association between the neurosecretory fibres and pars intermedia of the pituitary gland forming a neuro-intermediate lobe. Hypothalamo-Neurosecretory complex of Xenentodon cancila consists of Nucleus Pre-opticus (NPO), Nucleus Lateralis Tuberis (NLT) and their axonal tracts. NPO is a paired structure situated on either side of the third ventricle anterodorsal to the optic chiasma. NPO is divisible into a dorsal Pars Magnocellularis (PMC) consisting of large neurosecretory cells and Pars Parvocellularis (PPC) formed of smaller neurons. Neurons of PMC and PPC contribute beaded axons to form neurohypophysial tract. Herring bodies are seen in the anterior as well as posterior neurohypophysis.


Author(s):  
Morten H. Nielsen ◽  
Lone Bastholm

During the last 5 years the diameter of the gold probes used for immuno-cytochemical staining at the electron microscopical (EM) level has been decreased. The advantage of small diameter gold probes is an overall increased labelling density. The disadvantage is a lower detectability due to the low electron density of smaller gold particles consequently an inconvenient high primary magnification needed for EM examination. Since 1 nm gold particles are barely visible by conventional EM examination the need for enlargement by silverenhancement of the gold particles has increased.In the present study of ultrathin cryosectioned material the results of immunostaining using 5 nm gold conjugated antibody and 1 nm gold conjugated antibodies are compared after silverenhancement of the 1 nm gold particles.Slices of freshly isolated mouse pituitary gland were immersion fixed for 20 min in 2 % glutaraldehyde /2 % paraformaldehyde. Blocks cryoprotected with 2.3 M sucrose were frozen in liquid nitrogen and ultra-cryosectioned on a RMC cryoultra-microtome.


2015 ◽  
Vol 76 (S 01) ◽  
Author(s):  
Garni Barkhoudarian ◽  
Aaron Cutler ◽  
Sam Yost ◽  
Amy Eisenberg ◽  
Daniel Kelly

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