Cultivation of the early chick embryo in vitro

1943 ◽  
Vol 87 (4) ◽  
pp. 365-369 ◽  
Author(s):  
Alexis L. Romanoff
Keyword(s):  
Chick Embryo ◽  
Early Chick Embryo

Special Problems of Experimenting in ovo on the Early Chick Embryo, and a Solution

Development ◽  
1960 ◽  
Vol 8 (4) ◽  
pp. 369-375
Author(s):  
P. H. S. Silver
Keyword(s):  
Chick Embryo ◽  
Short Term ◽  
Stages Of Development ◽  
In Ovo ◽  
Technical Problems ◽  
Early Chick Embryo ◽  
In Vitro Methods ◽  
Early Stages

It seems to be generally accepted that experimenting in ovo on the chick during the early stages of development (up to about 48 hours) is fraught with the greatest difficulty. After about this time no serious technical problems arise and a high proportion of successful results can be expected. It is natural to ask why there should be this change-over from extreme difficulty to reasonable simplicity. New (1955) attributed to this ‘inaccessibility of the chick embryo in the egg’ the invention of his own and many other in vitro methods during the last 30 years. There is no doubt that, when short-term experiments only are required, in vitro methods will probably always be preferred. But all in vitro methods suffer from the disadvantage that the embryo cannot be expected to survive for more than 48 hours or so after explantation.

Differentiation induced by cyclic AMP in undifferentiated cells of early chick embryo in vitro

Nature ◽  
1976 ◽  
Vol 263 (5578) ◽  
pp. 588-591 ◽  
Author(s):  
A. K. DESHPANDE ◽  
M. A. Q. SIDDIQUI
Keyword(s):  
Chick Embryo ◽  
Cyclic Amp ◽  
Early Chick Embryo ◽  
Undifferentiated Cells

A STUDY OF THE INHIBITORY EFFECTS OF CHICK WHOLE EYE EXTRACT ON THE DEVELOPMENT OF THE LENS OF THE CHICK EMBRYO IN VITRO

1966 ◽  
Vol 44 (4) ◽  
pp. 661-676 ◽  
Author(s):  
Robert P. Thompson
Keyword(s):  
Chick Embryo ◽  
Nutrient Medium ◽  
Inhibitory Effect ◽  
Reactive System ◽  
Vesicle Formation ◽  
Inhibitory Effects ◽  
Muscle Extract ◽  
Lens Vesicle ◽  
And Control

To demonstrate the phenomenon of homologous inhibition by clearly interpretable results in a readily reactive system, experiments were carried out to study the effect of chick whole eye extract on the development of the vesicular lens of the chick embryo in vitro. The heads of embryos of 11 through 13 somites were explanted onto nutrient medium diluted with varying amounts of the extract, and cultured for 30 hours. A total of 35 embryos exposed to concentrations of 1:1, 1:2, and 1:4 (extract to medium) showed complete inhibition of lens vesicle formation. Of a total of 53 embryos on concentrations of 1:8, 1:16, 1:32, and 1:64, more than 50% showed inhibition of vesicle formation. The inhibitory effect disappeared at a concentration of 1:128. Control material exposed to some equivalent concentrations of nutrient medium – saline mixtures showed inhibition of vesicle formation in only 15% of 33 embryos. Of a total of 27 control embryos exposed to ventricular muscle extract, approximately one-third showed inhibition of vesicle formation at concentrations of 1:8 and 1:16, with the inhibitory effect disappearing at 1:32. The implications of this result are discussed. Other factors and control experiments are described and their value is assessed.

scholarly journals Identification of Novel Hemangioblast Genes in the Early Chick Embryo

Cells ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 9 ◽  
Author(s):  
José Serrado Marques ◽  
Vera Teixeira ◽  
António Jacinto ◽  
Ana Tavares
Keyword(s):  
Chick Embryo ◽  
Early Chick Embryo

Organ Antigen in the Early Chick Embryo

1948 ◽  
Vol 68 (2) ◽  
pp. 263-266 ◽  
Author(s):  
A. M. Schechtman
Keyword(s):  
Chick Embryo ◽  
Early Chick Embryo
Sign in / Sign up

Export Citation Format

Share Document