scholarly journals Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing

2019 ◽  
Vol 7 (1) ◽  
pp. e01208 ◽  
Author(s):  
Xinjia Wang ◽  
Li Zhong ◽  
Zhikun Wu ◽  
Huaying Sun ◽  
Hong Wang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Hiseq Sequencing

scholarly journals Characterization of 30 microsatellite markers for distylous Primula denticulata (Primulaceae) using HiSeq sequencing

2020 ◽  
Author(s):  
Hao-Pu Cao ◽  
Yu-Ting He ◽  
Li Zhong ◽  
Xin-Jia Wang ◽  
Spencer C. H. Barrett ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Hiseq Sequencing

Development and characterization of 24 microsatellite markers in Primula tosaensis , an endangered primrose, using MiSeq

2017 ◽  
Vol 33 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Masaya Yamamoto ◽  
Yoshihiro Handa ◽  
Hiroki Aihara ◽  
Hiroaki Setoguchi
Keyword(s):  
Microsatellite Markers

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattusL.)

2008 ◽  
Vol 8 (4) ◽  
pp. 861-863 ◽  
Author(s):  
T. DAILIANIS ◽  
M. LIMBORG ◽  
R. HANEL ◽  
D. BEKKEVOLD ◽  
J. LAGNEL ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite
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