scholarly journals Isolation and characterization of SSR and EST-SSR loci in Chamaecyparis formosensis (Cupressaceae)

2018 ◽  
Vol 6 (8) ◽  
pp. e01175 ◽  
Author(s):  
Chiun-Jr Huang ◽  
Fang-Hua Chu ◽  
Shau-Chian Liu ◽  
Yu-Hsin Tseng ◽  
Yi-Shiang Huang ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Ssr Loci ◽  
Chamaecyparis Formosensis

scholarly journals Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae)

2019 ◽  
Vol 7 (5) ◽  
pp. e01245
Author(s):  
Tamás Malkócs ◽  
Shyryn Almerekova ◽  
Judit Bereczki ◽  
Judit Cservenka ◽  
Emese Meglécz ◽  
...  
Keyword(s):  
Tetraploid Species ◽  
Isolation And Characterization ◽  
Ssr Loci

Isolation and characterization of β-cadinene synthase cDNA from Chamaecyparis formosensis Matsum

Holzforschung ◽  
2012 ◽  
Vol 66 (5) ◽  
pp. 569-576 ◽  
Author(s):  
Pei-Min Kuo ◽  
Keng-Hao Hsu ◽  
Yi-Ru Lee ◽  
Fang-Hua Chu ◽  
Sheng-Yang Wang
Keyword(s):  
Functional Expression ◽  
Major Product ◽  
Gas Chromatography Mass Spectrometry ◽  
Synthesis Mechanism ◽  
E Coli ◽  
Isolation And Characterization ◽  
Ms Analysis ◽  
Chamaecyparis Formosensis ◽  
Rapid Amplification

Abstract Chamaecyparis formosensis is a precious conifer endemic in Taiwan. To understand the sesquiterpene synthesis mechanism in this tree, full-length cDNA of a putative sesquiterpene synthase (sesqui-TPS), designated Cf-Cad, was obtained by rapid amplification of complementary DNA ends-polymerase chain reaction. Cf-Cad is 1812 bp in length. To identify its function, recombinant protein from Escherichia coli was incubated with farnesyl diphosphate, which produced one major product, the structure of which was elucidated by gas chromatography/mass spectrometry (GC/MS) analysis. GC/MS analysis, GC retention time and MS matching with authentic standards revealed that the major product was β-cadinene. This is the first report of the cloning, functional expression in E. coli and identification of a sesqui-TPS from a Cupressaceae conifer.

Isolation and characterization of 39 novel polymorphic EST-SSR loci for the blood clam, Tegillarca granosa

2011 ◽  
Vol 4 (2) ◽  
pp. 375-378 ◽  
Author(s):  
Bo Liu ◽  
Shuang-shuang Teng ◽  
Yan-Qing Shao ◽  
Xue-Liang Chai ◽  
Guo-Qiang Xiao
Keyword(s):  
Tegillarca Granosa ◽  
Isolation And Characterization ◽  
Blood Clam ◽  
Ssr Loci

Isolation and characterization of 28 polymorphic SSR loci from castor bean (Ricinus communis L.)

2011 ◽  
Vol 14 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Kyoung-In Seo ◽  
Gi-An Lee ◽  
Kyung-Ho Ma ◽  
Do-Yoon Hyun ◽  
Yong-Jin Park ◽  
...  
Keyword(s):  
Castor Bean ◽  
Ricinus Communis ◽  
Isolation And Characterization ◽  
Ricinus Communis L ◽  
Ssr Loci

scholarly journals Isolation and Characterization of Microsatellites Markers in Centaurium Grandiflorum ssp. Boissieri

2021 ◽  
Author(s):  
Vania Jiménez-Lobato ◽  
Marcial Escudero ◽  
Zoila Díaz Lifante ◽  
Cristina Andrés Camacho ◽  
Alejandra de Castro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mating System ◽  
Flower Morphology ◽  
System Parameters ◽  
Microsatellites Markers ◽  
Isolation And Characterization ◽  
Ssr Loci ◽  
Hardy Weinberg Equilibrium ◽  
Two Populations

Abstract Estimation of outcrossing/selfing rates and characterization of genetic diversity with microsatellite markers are crucial to understand the evolution of mating system in plant species. We developed, optimized and characterized eight new primers pairs for Centaurium grandiflorum ssp. boissieri and transferred them to three subspecies of Centaurium quadrifolium. Two SSR loci were transferred from Sabatia campestris to the four mentioned taxa of Centaurium. Polymorphisms, He, Ho and H-W deviations were estimated in two populations of C. grandiflorum ssp. boissieri, and in seven individuals of C. quadrifolium ssp. barrelieri, C. quadrifolium ssp. parviflorum and C. quadrifolium ssp. quadrifolium. A total of 80 individuals were used in these experiments. The number of polymorphic loci varied among species from one to ten. A total number of 127 alleles were scored. The average number of alleles per locus was 12.7. He was higher than Ho in all sampled populations. Hardy-Weinberg equilibrium was found for some loci in different species. This is the first report of microsatellites successfully amplified in the whole Centaurium genus. They will be valuable for estimation of mating system parameters, genetic diversity and explore its relationship with the wide flower morphology, especially anther-stigma separation, found along the genus.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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